FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2073301981> ?p ?o ?g. }

• W2073301981 endingPage "13" @default.
• W2073301981 startingPage "8" @default.
• W2073301981 abstract "dUTPases are essential to eliminate dUTP for DNA integrity and provide dUMP for thymidylate biosynthesis. Mycobacterium tuberculosis apparently lacks any other thymidylate biosynthesis pathway, therefore dUTPase is a promising antituberculotic drug target. Crystal structure of the mycobacterial enzyme in complex with the isosteric substrate analog, α,β-imido-dUTP and Mg2+ at 1.5 Å resolution was determined that visualizes the full-length C-terminus, previously not localized. Interactions of a conserved motif important in catalysis, the Mycobacterium-specific five-residue-loop insert and C-terminal tetrapeptide could now be described in detail. Stacking of C-terminal histidine upon the uracil moiety prompted replacement with tryptophan. The resulting sensitive fluorescent sensor enables fast screening for binding of potential inhibitors to the active site. Kd for α,β-imido-dUTP binding to mycobacterial dUTPase is determined to be 10-fold less than for human dUTPase, which is to be considered in drug optimization. A robust continuous activity assay for kinetic screening is proposed." @default.
• W2073301981 created "2016-06-24" @default.
• W2073301981 creator A5004676852 @default.
• W2073301981 creator A5008774671 @default.
• W2073301981 creator A5018719528 @default.
• W2073301981 creator A5039162140 @default.
• W2073301981 creator A5040342860 @default.
• W2073301981 creator A5064012236 @default.
• W2073301981 date "2008-08-01" @default.
• W2073301981 modified "2023-10-17" @default.
• W2073301981 title "Active site of mycobacterial dUTPase: Structural characteristics and a built-in sensor" @default.
• W2073301981 cites W1964643332 @default.
• W2073301981 cites W1971527860 @default.
• W2073301981 cites W1975492167 @default.
• W2073301981 cites W1980595371 @default.
• W2073301981 cites W2024321664 @default.
• W2073301981 cites W2029584311 @default.
• W2073301981 cites W2033923013 @default.
• W2073301981 cites W2035786230 @default.
• W2073301981 cites W2039500097 @default.
• W2073301981 cites W2044557793 @default.
• W2073301981 cites W2050211725 @default.
• W2073301981 cites W2062271236 @default.
• W2073301981 cites W2067688949 @default.
• W2073301981 cites W2077253846 @default.
• W2073301981 cites W2090612467 @default.
• W2073301981 cites W2093352973 @default.
• W2073301981 cites W2097493124 @default.
• W2073301981 cites W2107105825 @default.
• W2073301981 cites W2114333751 @default.
• W2073301981 cites W2144010669 @default.
• W2073301981 cites W2156007695 @default.
• W2073301981 cites W2162256026 @default.
• W2073301981 cites W4244238901 @default.
• W2073301981 cites W4320995821 @default.
• W2073301981 doi "https://doi.org/10.1016/j.bbrc.2008.05.130" @default.
• W2073301981 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/18519027" @default.
• W2073301981 hasPublicationYear "2008" @default.
• W2073301981 type Work @default.
• W2073301981 sameAs 2073301981 @default.
• W2073301981 citedByCount "60" @default.
• W2073301981 countsByYear W20733019812012 @default.
• W2073301981 countsByYear W20733019812013 @default.
• W2073301981 countsByYear W20733019812014 @default.
• W2073301981 countsByYear W20733019812015 @default.
• W2073301981 countsByYear W20733019812016 @default.
• W2073301981 countsByYear W20733019812017 @default.
• W2073301981 countsByYear W20733019812018 @default.
• W2073301981 countsByYear W20733019812019 @default.
• W2073301981 countsByYear W20733019812020 @default.
• W2073301981 countsByYear W20733019812021 @default.
• W2073301981 countsByYear W20733019812023 @default.
• W2073301981 crossrefType "journal-article" @default.
• W2073301981 hasAuthorship W2073301981A5004676852 @default.
• W2073301981 hasAuthorship W2073301981A5008774671 @default.
• W2073301981 hasAuthorship W2073301981A5018719528 @default.
• W2073301981 hasAuthorship W2073301981A5039162140 @default.
• W2073301981 hasAuthorship W2073301981A5040342860 @default.
• W2073301981 hasAuthorship W2073301981A5064012236 @default.
• W2073301981 hasConcept C104317684 @default.
• W2073301981 hasConcept C107824862 @default.
• W2073301981 hasConcept C123321153 @default.
• W2073301981 hasConcept C181199279 @default.
• W2073301981 hasConcept C185592680 @default.
• W2073301981 hasConcept C2776694085 @default.
• W2073301981 hasConcept C2778460671 @default.
• W2073301981 hasConcept C2779281246 @default.
• W2073301981 hasConcept C2779564779 @default.
• W2073301981 hasConcept C2780456651 @default.
• W2073301981 hasConcept C38441620 @default.
• W2073301981 hasConcept C41183919 @default.
• W2073301981 hasConcept C54355233 @default.
• W2073301981 hasConcept C552990157 @default.
• W2073301981 hasConcept C553450214 @default.
• W2073301981 hasConcept C55493867 @default.
• W2073301981 hasConcept C67705224 @default.
• W2073301981 hasConcept C71240020 @default.
• W2073301981 hasConcept C86803240 @default.
• W2073301981 hasConceptScore W2073301981C104317684 @default.
• W2073301981 hasConceptScore W2073301981C107824862 @default.
• W2073301981 hasConceptScore W2073301981C123321153 @default.
• W2073301981 hasConceptScore W2073301981C181199279 @default.
• W2073301981 hasConceptScore W2073301981C185592680 @default.
• W2073301981 hasConceptScore W2073301981C2776694085 @default.
• W2073301981 hasConceptScore W2073301981C2778460671 @default.
• W2073301981 hasConceptScore W2073301981C2779281246 @default.
• W2073301981 hasConceptScore W2073301981C2779564779 @default.
• W2073301981 hasConceptScore W2073301981C2780456651 @default.
• W2073301981 hasConceptScore W2073301981C38441620 @default.
• W2073301981 hasConceptScore W2073301981C41183919 @default.
• W2073301981 hasConceptScore W2073301981C54355233 @default.
• W2073301981 hasConceptScore W2073301981C552990157 @default.
• W2073301981 hasConceptScore W2073301981C553450214 @default.
• W2073301981 hasConceptScore W2073301981C55493867 @default.
• W2073301981 hasConceptScore W2073301981C67705224 @default.
• W2073301981 hasConceptScore W2073301981C71240020 @default.
• W2073301981 hasConceptScore W2073301981C86803240 @default.
• W2073301981 hasIssue "1" @default.

• next