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- W2073353663 abstract "Highly sensitive and selective FIA flow injection analysis procedures for the determination of sulfite/hydrogen sulfite/sulfur dioxide were developed on the basis of an in situ-generated o-phthalaldehyde (OPA)/ammonium reagent and fluorescence detection. The highest sensitivity was achieved at an excitation wavelength of 330 nm, an emission wavelength of 390 nm, and at pH 6.5. Sulfite concentrations between 2.5 nM and 5 μM can be determined with relative standard deviations between 10.5 and 1.0% (n = 5, confidence level α = 0.05) by utilization of a reagent that contains 0.2 mM OPA and 0.4 M NH4Cl in 50 mM potassium phosphate buffer. A concentration of 0.1 mM sulfite can be selectively detected in the presence of thiosulfate, thioglycolate, tetrathionate, cysteine, and ascorbate. The fluorometric sulfite detection was combined with a membrane gas diffusion step to improve the selectivity with respect to nonvolatile fluorescing substances. The total sulfite content can be quantitatively separated as sulfur dioxide into an acceptor solution before its flow detection. Between 40 nM and 0.1 mM sulfite can be determined. After 1000-fold dilution, the total sulfite content can be determined in white and red wines." @default.
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- W2073353663 date "2001-05-25" @default.
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- W2073353663 title "Sensitive and Selective Flow Injection Analysis of Hydrogen Sulfite/Sulfur Dioxide by Fluorescence Detection with and without Membrane Separation by Gas Diffusion" @default.
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- W2073353663 doi "https://doi.org/10.1021/ac001049q" @default.
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