Matches in SemOpenAlex for { <https://semopenalex.org/work/W2073359632> ?p ?o ?g. }
Showing items 1 to 93 of
93
with 100 items per page.
- W2073359632 endingPage "44" @default.
- W2073359632 startingPage "41" @default.
- W2073359632 abstract "RNA-based molecular technique (RT-qPCR) is a promising method for microcystin monitoring in lakes and reservoirs, but great lability of RNA in cyanobacterial samples limits its application. To date, no studies have investigated how to effectively preserve RNA in cyanobacterial samples. In this study, four different treatments (−80 °C freezer, −196 °C liquid nitrogen, 4 °C or 25 °C preservation after adding RNA protective fluid) were employed to preserve RNA in pure culture and field Microcystis samples, and RNA degradation in these treatments were systematically evaluated. Results showed liquid nitrogen was the most effective treatment to preserve RNA in pure culture and field Microcystis samples. RNA preservation using RNA protective fluid was temperature dependent. Low temperature (4 °C) could effectively slow down RNA degradation within a short time (1–7 d), since decay rate of mcyH mRNA (k = 0.00094 d−1) was much lower at 4 °C than that at 25 °C (0.0549 d−1) (P < 0.05). However, for field samples, RNA degradation was much faster than pure culture samples with the same treatment. Therefore, to better preserve RNA in field samples, a practical strategy for RNA preservation combining RNA protective fluid and liquid nitrogen, was proposed. Tests of field experiments showed it was more effective than individual treatment for RNA preservation in Microcystis samples during field sampling. Thus, this strategy could be employed to preserve RNA in cyanobacterial samples during field sampling, which will contribute to the application of RT-qPCR technique for microcystin monitoring in lakes and reservoirs." @default.
- W2073359632 created "2016-06-24" @default.
- W2073359632 creator A5072609985 @default.
- W2073359632 creator A5081899613 @default.
- W2073359632 date "1985-01-01" @default.
- W2073359632 modified "2023-09-27" @default.
- W2073359632 title "The fine structure and chemical composition of the cell wall and sheath layers of cyanobacteria" @default.
- W2073359632 cites W1507280750 @default.
- W2073359632 cites W1625885638 @default.
- W2073359632 cites W1645069563 @default.
- W2073359632 cites W1963946970 @default.
- W2073359632 cites W2016267544 @default.
- W2073359632 cites W2057117059 @default.
- W2073359632 cites W2069624364 @default.
- W2073359632 cites W2078903953 @default.
- W2073359632 cites W2127881473 @default.
- W2073359632 cites W2128129953 @default.
- W2073359632 doi "https://doi.org/10.1016/s0769-2609(85)80019-3" @default.
- W2073359632 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/3923903" @default.
- W2073359632 hasPublicationYear "1985" @default.
- W2073359632 type Work @default.
- W2073359632 sameAs 2073359632 @default.
- W2073359632 citedByCount "30" @default.
- W2073359632 countsByYear W20733596322012 @default.
- W2073359632 countsByYear W20733596322014 @default.
- W2073359632 countsByYear W20733596322015 @default.
- W2073359632 countsByYear W20733596322016 @default.
- W2073359632 countsByYear W20733596322018 @default.
- W2073359632 countsByYear W20733596322019 @default.
- W2073359632 countsByYear W20733596322020 @default.
- W2073359632 crossrefType "journal-article" @default.
- W2073359632 hasAuthorship W2073359632A5072609985 @default.
- W2073359632 hasAuthorship W2073359632A5081899613 @default.
- W2073359632 hasConcept C104317684 @default.
- W2073359632 hasConcept C105580179 @default.
- W2073359632 hasConcept C112190416 @default.
- W2073359632 hasConcept C153911025 @default.
- W2073359632 hasConcept C178790620 @default.
- W2073359632 hasConcept C185592680 @default.
- W2073359632 hasConcept C2776056151 @default.
- W2073359632 hasConcept C2779669040 @default.
- W2073359632 hasConcept C2779679103 @default.
- W2073359632 hasConcept C2781226839 @default.
- W2073359632 hasConcept C41008148 @default.
- W2073359632 hasConcept C43617362 @default.
- W2073359632 hasConcept C523546767 @default.
- W2073359632 hasConcept C537208039 @default.
- W2073359632 hasConcept C54355233 @default.
- W2073359632 hasConcept C55493867 @default.
- W2073359632 hasConcept C67705224 @default.
- W2073359632 hasConcept C76155785 @default.
- W2073359632 hasConcept C86803240 @default.
- W2073359632 hasConceptScore W2073359632C104317684 @default.
- W2073359632 hasConceptScore W2073359632C105580179 @default.
- W2073359632 hasConceptScore W2073359632C112190416 @default.
- W2073359632 hasConceptScore W2073359632C153911025 @default.
- W2073359632 hasConceptScore W2073359632C178790620 @default.
- W2073359632 hasConceptScore W2073359632C185592680 @default.
- W2073359632 hasConceptScore W2073359632C2776056151 @default.
- W2073359632 hasConceptScore W2073359632C2779669040 @default.
- W2073359632 hasConceptScore W2073359632C2779679103 @default.
- W2073359632 hasConceptScore W2073359632C2781226839 @default.
- W2073359632 hasConceptScore W2073359632C41008148 @default.
- W2073359632 hasConceptScore W2073359632C43617362 @default.
- W2073359632 hasConceptScore W2073359632C523546767 @default.
- W2073359632 hasConceptScore W2073359632C537208039 @default.
- W2073359632 hasConceptScore W2073359632C54355233 @default.
- W2073359632 hasConceptScore W2073359632C55493867 @default.
- W2073359632 hasConceptScore W2073359632C67705224 @default.
- W2073359632 hasConceptScore W2073359632C76155785 @default.
- W2073359632 hasConceptScore W2073359632C86803240 @default.
- W2073359632 hasIssue "1" @default.
- W2073359632 hasLocation W20733596321 @default.
- W2073359632 hasLocation W20733596322 @default.
- W2073359632 hasOpenAccess W2073359632 @default.
- W2073359632 hasPrimaryLocation W20733596321 @default.
- W2073359632 hasRelatedWork W1964019338 @default.
- W2073359632 hasRelatedWork W1994717423 @default.
- W2073359632 hasRelatedWork W2001473932 @default.
- W2073359632 hasRelatedWork W2067597777 @default.
- W2073359632 hasRelatedWork W2073556641 @default.
- W2073359632 hasRelatedWork W2162475123 @default.
- W2073359632 hasRelatedWork W2317649254 @default.
- W2073359632 hasRelatedWork W2580790768 @default.
- W2073359632 hasRelatedWork W2966791134 @default.
- W2073359632 hasRelatedWork W3162254187 @default.
- W2073359632 hasVolume "136" @default.
- W2073359632 isParatext "false" @default.
- W2073359632 isRetracted "false" @default.
- W2073359632 magId "2073359632" @default.
- W2073359632 workType "article" @default.