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• W2073384172 abstract "Materials with tailored surface properties are useful for diverse applications, in particular for adsorption and/or covalent immobilization of various biomolecules such as nucleic acids and proteins. One of the main challenges is to design a reactive surface chemistry for stable binding of biomolecules on the support, which in addition keeps away the biomolecules from the support to reduce interference. We report here a novel support functionalization that is based on the formation of a reactive layer, covalently grafted on glass slides onto which amino-modified DNA probes were covalently fixed. The layer is composed of spherical home-made neutral phosphorus dendrimers containing a large number of aldehyde functions at their periphery. The method of preparation of these dendrimer-activated glass slides (so-called dendrislides) is performed in a few steps and results in stable activated supports, since aldehyde dendrimers are stable compounds. After immobilization of nucleic acids the so-called dendrichip products were investigated by means of hybridization experiments using complementary fluorescent labelled-oligonucleotide targets. Our results indicate that this novel grafting technology leads to surfaces with a high binding capacity for amino-modified oligonucleotides compared to commercially available aldehyde slides and with a detection limit of 1 pM labelled targets. Since links between the surface, the dendrimers and the nucleic acids are covalent, the dendrichips could be re-used up to 10 times without significant change in the fluorescence signal intensity. Finally, the performance of dendrichips in detection of a single base mutation was demonstrated." @default.
• W2073384172 created "2016-06-24" @default.
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• W2073384172 date "2003-01-01" @default.
• W2073384172 modified "2023-10-17" @default.
• W2073384172 title "Dendrislides, dendrichips: a simple chemical functionalization of glass slides with phosphorus dendrimers as an effective means for the preparation of biochips" @default.
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• W2073384172 doi "https://doi.org/10.1039/b307928g" @default.
• W2073384172 hasPublicationYear "2003" @default.
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