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- W2073422183 abstract "The technique of difference spectroscopy has been utilized to investigate the structural alterations in the thylakoid membranes induced by membrane perturbants such as temperature and benzyl alcohol. Upon cooling and heating of the thylakoid membranes, major Δ A bands were observed at 495–498 and 678–680nm in the absence of benzyl alcohol, and at 500 – 503 and 678 – 680nm in the presence of 60mM benzyl alcohol. During ambient→cooling cycle, a negative band observed at 690nm in the untreated chloroplasts was absent in the benzyl alcohol-treated chloroplasts and, in general, the Δ A changes were significantly higher in the benzyl alcohol-treated chloroplasts compared to the untreated chloroplasts. For example, during ambient→heating cycle, the maximum absorption changes were 5.3 and 13.3% in the untreated and benzyl alcohol-treated chloroplasts, respectively. Reversibility of the absorbance changes was almost complete in the untreated chloroplasts whereas it was only 26–42% in the benzyl alcohol-treated chloroplasts. Upon cooling of the heated benzyl alcohol-treated chloroplasts to ambient temperature, a sharp positive ΔA band appeared at 710nm which was not observed in the untreated chloroplasts. This indicated an aggregation of chlorophyll molecules resulting from reorganization of the chloroplast membranes. Based on the results, it appears that the membrane perturbation by both temperature and benzyl alcohol causes differential structural alterations in the chloroplast membranes, and membrane fluidity may play an important role in the chlorophyll aggregation." @default.
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- W2073422183 date "1990-01-01" @default.
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- W2073422183 title "Spectral Changes in Barley Chloroplast Membranes Induced by a Combination of Temperature and a Membrane Perturbant" @default.
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- W2073422183 doi "https://doi.org/10.1016/s0015-3796(11)80284-2" @default.
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