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• W2073428610 abstract "This study presents a microfabricated device for polymerase chain reaction (PCR) with a flow-through manner for use in environmental microbiology. The device was developed utilizing photolithography and softlithography techniques and evaluated as one component of a totally integrated in situ gene analysis system. The developed device was composed of a glass-based “temperature control chip” and polydimethylsiloxane (PDMS)-based “microchannel chip”. For the temperature control chip, six heaters made from indium tin oxide (ITO) are placed on a glass substrate to define three uniform temperature zones for flow-through PCR. On the each heater, a platinum (Pt) line was placed as a temperature sensor. The PDMS microchannel structure was fabricated by using a molding method with a negatively patterned mold master. The width and depth of folded microchannel was 100 μm and total length for 30 cycles of flow-through PCR was approximately 3.0 m. With the flow-through PCR device, 580 and 1450 bp (base pairs) of DNA fragments were successfully amplified from Escherichia coli genomic DNA and directly from untreated cells." @default.
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• W2073428610 date "2004-08-01" @default.
• W2073428610 modified "2023-10-01" @default.
• W2073428610 title "Microfabricated flow-through device for DNA amplification—towards in situ gene analysis" @default.
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• W2073428610 doi "https://doi.org/10.1016/j.cej.2003.11.016" @default.
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