Matches in SemOpenAlex for { <https://semopenalex.org/work/W2073438676> ?p ?o ?g. }
Showing items 1 to 99 of
99
with 100 items per page.
- W2073438676 endingPage "19" @default.
- W2073438676 startingPage "11" @default.
- W2073438676 abstract "Two soluble forms of α-mannosidase (I and II) were purified approximately 1000-fold to electrophoretic homogeneity from sunflower hypocotyls by a procedure involving ammonium sulfate fractionation, gel filtration and anion-exchange chromatographies, and chromatofocusing. A minute amount (approximately 5% of the total purified α-mannosidase activity) of a possible third form was also detected upon chromatofocusing and was not further studied. The main I and II forms were characterized and showed the following properties: (1) glycoprotein nature; (2) apparent molecular weight of 260 000 (gel filtration); (3) pH optimum of 4.4 and isoelectric points of 4.7 (I) and 5.3 (II); (4) temperature optimum of approximately 50 °C and apparent activation energies of 32 kJ/mol (I) and 38 kJ/mol (II); (5) absolute Zn2+ requirement for activity; (6) Km and Vm values of 3.7 mM and 3.3 μkat/mg protein (I), and 2.7 mM and 3.7 μkat/mg protein (II)." @default.
- W2073438676 created "2016-06-24" @default.
- W2073438676 creator A5000697183 @default.
- W2073438676 creator A5023064696 @default.
- W2073438676 creator A5034346786 @default.
- W2073438676 creator A5089934074 @default.
- W2073438676 date "1989-01-01" @default.
- W2073438676 modified "2023-09-25" @default.
- W2073438676 title "Purification and partial characterization of soluble α-mannosidase isoforms from sunflower (Helianthus annuus L.) hypocotyls" @default.
- W2073438676 cites W1585085187 @default.
- W2073438676 cites W1625660792 @default.
- W2073438676 cites W191542959 @default.
- W2073438676 cites W1990141910 @default.
- W2073438676 cites W1994588407 @default.
- W2073438676 cites W1995987439 @default.
- W2073438676 cites W1999996816 @default.
- W2073438676 cites W2006613155 @default.
- W2073438676 cites W2029368679 @default.
- W2073438676 cites W2032799408 @default.
- W2073438676 cites W2038345597 @default.
- W2073438676 cites W2052539095 @default.
- W2073438676 cites W2052700765 @default.
- W2073438676 cites W2064938399 @default.
- W2073438676 cites W2074742656 @default.
- W2073438676 cites W2084266556 @default.
- W2073438676 cites W2089541873 @default.
- W2073438676 cites W2110671059 @default.
- W2073438676 cites W2111301002 @default.
- W2073438676 cites W2121307894 @default.
- W2073438676 cites W2137302959 @default.
- W2073438676 cites W2152708819 @default.
- W2073438676 cites W2204254819 @default.
- W2073438676 cites W2212445342 @default.
- W2073438676 cites W2218138196 @default.
- W2073438676 cites W2463164778 @default.
- W2073438676 cites W2911547482 @default.
- W2073438676 cites W3025108290 @default.
- W2073438676 cites W4293247451 @default.
- W2073438676 doi "https://doi.org/10.1016/0168-9452(89)90183-0" @default.
- W2073438676 hasPublicationYear "1989" @default.
- W2073438676 type Work @default.
- W2073438676 sameAs 2073438676 @default.
- W2073438676 citedByCount "2" @default.
- W2073438676 crossrefType "journal-article" @default.
- W2073438676 hasAuthorship W2073438676A5000697183 @default.
- W2073438676 hasAuthorship W2073438676A5023064696 @default.
- W2073438676 hasAuthorship W2073438676A5034346786 @default.
- W2073438676 hasAuthorship W2073438676A5089934074 @default.
- W2073438676 hasConcept C181199279 @default.
- W2073438676 hasConcept C185592680 @default.
- W2073438676 hasConcept C193363816 @default.
- W2073438676 hasConcept C2776428949 @default.
- W2073438676 hasConcept C2776829962 @default.
- W2073438676 hasConcept C2778181364 @default.
- W2073438676 hasConcept C2779197568 @default.
- W2073438676 hasConcept C43617362 @default.
- W2073438676 hasConcept C50476208 @default.
- W2073438676 hasConcept C55493867 @default.
- W2073438676 hasConcept C58121356 @default.
- W2073438676 hasConcept C6557445 @default.
- W2073438676 hasConcept C86803240 @default.
- W2073438676 hasConcept C96294017 @default.
- W2073438676 hasConcept C97428945 @default.
- W2073438676 hasConceptScore W2073438676C181199279 @default.
- W2073438676 hasConceptScore W2073438676C185592680 @default.
- W2073438676 hasConceptScore W2073438676C193363816 @default.
- W2073438676 hasConceptScore W2073438676C2776428949 @default.
- W2073438676 hasConceptScore W2073438676C2776829962 @default.
- W2073438676 hasConceptScore W2073438676C2778181364 @default.
- W2073438676 hasConceptScore W2073438676C2779197568 @default.
- W2073438676 hasConceptScore W2073438676C43617362 @default.
- W2073438676 hasConceptScore W2073438676C50476208 @default.
- W2073438676 hasConceptScore W2073438676C55493867 @default.
- W2073438676 hasConceptScore W2073438676C58121356 @default.
- W2073438676 hasConceptScore W2073438676C6557445 @default.
- W2073438676 hasConceptScore W2073438676C86803240 @default.
- W2073438676 hasConceptScore W2073438676C96294017 @default.
- W2073438676 hasConceptScore W2073438676C97428945 @default.
- W2073438676 hasIssue "1" @default.
- W2073438676 hasLocation W20734386761 @default.
- W2073438676 hasOpenAccess W2073438676 @default.
- W2073438676 hasPrimaryLocation W20734386761 @default.
- W2073438676 hasRelatedWork W1508911271 @default.
- W2073438676 hasRelatedWork W1606562654 @default.
- W2073438676 hasRelatedWork W1995303931 @default.
- W2073438676 hasRelatedWork W2010626519 @default.
- W2073438676 hasRelatedWork W2073438676 @default.
- W2073438676 hasRelatedWork W2261481815 @default.
- W2073438676 hasRelatedWork W2361605433 @default.
- W2073438676 hasRelatedWork W2412767439 @default.
- W2073438676 hasRelatedWork W2469169917 @default.
- W2073438676 hasRelatedWork W4253927048 @default.
- W2073438676 hasVolume "62" @default.
- W2073438676 isParatext "false" @default.
- W2073438676 isRetracted "false" @default.
- W2073438676 magId "2073438676" @default.
- W2073438676 workType "article" @default.