FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2073443103> ?p ?o ?g. }

• W2073443103 endingPage "7552" @default.
• W2073443103 startingPage "7542" @default.
• W2073443103 abstract "IF1, the natural inhibitor protein of FOF1ATP synthase able to regulate the ATP hydrolytic activity of both mitochondrial and cell surface enzyme, exists in two oligomeric states depending on pH: an inactive, highly helical, tetrameric form above pH 6.7 and an active, inhibitory, dimeric form below pH 6.7 [Cabezon, E., Butler, P. J., Runswick, M. J., and Walker, J. E. (2000) J. Biol. Chem. 275, 25460−25464]. IF1 is known to interact in vitro with the archetypal EF-hand calcium sensor calmodulin (CaM), as well to colocalize with CaM on the plasma membrane of cultured cells. Low resolution structural data were herein obtained in order to get insights into the molecular interaction between IF1 and CaM. A combined structural proteomic strategy was used which integrates limited proteolysis and chemical cross-linking with mass spectrometric analysis. Specifically, chemical cross-linking data clearly indicate that the C-terminal lobe of CaM molecule contacts IF1 within the inhibitory, flexible N-terminal region that is not involved in the dimeric interface in IF1. Nevertheless, native mass spectrometry analysis demonstrated that in the micromolar range the stoichiometry of the IF1−CaM complex is 1:1, thereby indicating that binding to CaM promotes IF1 dimer dissociation without directly interfering with the intersubunit contacts of the IF1 dimer. The relevance of the finding that only the C-terminal lobe of CaM is involved in the interaction is two fold: (i) the IF1−CaM complex can be included in the category of noncanonical structures of CaM complexes; (ii) it can be inferred that the N-terminal region of CaM might have the opportunity to bind to a second target." @default.
• W2073443103 created "2016-06-24" @default.
• W2073443103 creator A5000638234 @default.
• W2073443103 creator A5019815285 @default.
• W2073443103 creator A5037929317 @default.
• W2073443103 creator A5047963189 @default.
• W2073443103 creator A5061352289 @default.
• W2073443103 creator A5069728887 @default.
• W2073443103 creator A5081117764 @default.
• W2073443103 date "2010-08-16" @default.
• W2073443103 modified "2023-09-23" @default.
• W2073443103 title "Stoichiometry and Topology of the Complex of the Endogenous ATP Synthase Inhibitor Protein IF₁ with Calmodulin" @default.
• W2073443103 cites W1576403707 @default.
• W2073443103 cites W1599066318 @default.
• W2073443103 cites W1767701883 @default.
• W2073443103 cites W1968901680 @default.
• W2073443103 cites W1974607158 @default.
• W2073443103 cites W1980146060 @default.
• W2073443103 cites W1996333756 @default.
• W2073443103 cites W1997297151 @default.
• W2073443103 cites W2001535102 @default.
• W2073443103 cites W2008166558 @default.
• W2073443103 cites W2023665029 @default.
• W2073443103 cites W2024142216 @default.
• W2073443103 cites W2030302226 @default.
• W2073443103 cites W2030490793 @default.
• W2073443103 cites W2030890665 @default.
• W2073443103 cites W2031811477 @default.
• W2073443103 cites W2033078564 @default.
• W2073443103 cites W2044228723 @default.
• W2073443103 cites W2054319504 @default.
• W2073443103 cites W2062520829 @default.
• W2073443103 cites W2065031102 @default.
• W2073443103 cites W2067422561 @default.
• W2073443103 cites W2071163862 @default.
• W2073443103 cites W2079249370 @default.
• W2073443103 cites W2084344997 @default.
• W2073443103 cites W2089114600 @default.
• W2073443103 cites W2090625386 @default.
• W2073443103 cites W2095188039 @default.
• W2073443103 cites W2096283154 @default.
• W2073443103 cites W2096716204 @default.
• W2073443103 cites W2096942328 @default.
• W2073443103 cites W2099666638 @default.
• W2073443103 cites W2120385383 @default.
• W2073443103 cites W2133380824 @default.
• W2073443103 cites W2141378441 @default.
• W2073443103 cites W2142396509 @default.
• W2073443103 cites W2147909914 @default.
• W2073443103 cites W2168398034 @default.
• W2073443103 cites W4254386665 @default.
• W2073443103 doi "https://doi.org/10.1021/bi100447t" @default.
• W2073443103 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/20669893" @default.
• W2073443103 hasPublicationYear "2010" @default.
• W2073443103 type Work @default.
• W2073443103 sameAs 2073443103 @default.
• W2073443103 citedByCount "7" @default.
• W2073443103 countsByYear W20734431032012 @default.
• W2073443103 countsByYear W20734431032013 @default.
• W2073443103 countsByYear W20734431032014 @default.
• W2073443103 countsByYear W20734431032016 @default.
• W2073443103 countsByYear W20734431032017 @default.
• W2073443103 countsByYear W20734431032022 @default.
• W2073443103 crossrefType "journal-article" @default.
• W2073443103 hasAuthorship W2073443103A5000638234 @default.
• W2073443103 hasAuthorship W2073443103A5019815285 @default.
• W2073443103 hasAuthorship W2073443103A5037929317 @default.
• W2073443103 hasAuthorship W2073443103A5047963189 @default.
• W2073443103 hasAuthorship W2073443103A5061352289 @default.
• W2073443103 hasAuthorship W2073443103A5069728887 @default.
• W2073443103 hasAuthorship W2073443103A5081117764 @default.
• W2073443103 hasConcept C112243037 @default.
• W2073443103 hasConcept C12554922 @default.
• W2073443103 hasConcept C141315368 @default.
• W2073443103 hasConcept C178790620 @default.
• W2073443103 hasConcept C181199279 @default.
• W2073443103 hasConcept C185592680 @default.
• W2073443103 hasConcept C23265538 @default.
• W2073443103 hasConcept C2779546866 @default.
• W2073443103 hasConcept C2781142959 @default.
• W2073443103 hasConcept C2781307694 @default.
• W2073443103 hasConcept C29688787 @default.
• W2073443103 hasConcept C40209533 @default.
• W2073443103 hasConcept C55493867 @default.
• W2073443103 hasConcept C71240020 @default.
• W2073443103 hasConcept C86803240 @default.
• W2073443103 hasConcept C95444343 @default.
• W2073443103 hasConceptScore W2073443103C112243037 @default.
• W2073443103 hasConceptScore W2073443103C12554922 @default.
• W2073443103 hasConceptScore W2073443103C141315368 @default.
• W2073443103 hasConceptScore W2073443103C178790620 @default.
• W2073443103 hasConceptScore W2073443103C181199279 @default.
• W2073443103 hasConceptScore W2073443103C185592680 @default.
• W2073443103 hasConceptScore W2073443103C23265538 @default.
• W2073443103 hasConceptScore W2073443103C2779546866 @default.
• W2073443103 hasConceptScore W2073443103C2781142959 @default.
• W2073443103 hasConceptScore W2073443103C2781307694 @default.
• W2073443103 hasConceptScore W2073443103C29688787 @default.

• next