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• W2073470794 endingPage "e1000383" @default.
• W2073470794 startingPage "e1000383" @default.
• W2073470794 abstract "The malarial parasite Plasmodium falciparum possesses a functional thioredoxin and glutathione system comprising the dithiol-containing redox proteins thioredoxin (Trx) and glutaredoxin (Grx), as well as plasmoredoxin (Plrx), which is exclusively found in Plasmodium species. All three proteins belong to the thioredoxin superfamily and share a conserved Cys-X-X-Cys motif at the active site. Only a few of their target proteins, which are likely to be involved in redox reactions, are currently known. The aim of the present study was to extend our knowledge of the Trx-, Grx-, and Plrx-interactome in Plasmodium. Based on the reaction mechanism, we generated active site mutants of Trx and Grx lacking the resolving cysteine residue. These mutants were bound to affinity columns to trap target proteins from P. falciparum cell extracts after formation of intermolecular disulfide bonds. Covalently linked proteins were eluted with dithiothreitol and analyzed by mass spectrometry. For Trx and Grx, we were able to isolate 17 putatively redox-regulated proteins each. Furthermore, the approach was successfully established for Plrx, leading to the identification of 21 potential target proteins. In addition to confirming known interaction partners, we captured potential target proteins involved in various processes including protein biosynthesis, energy metabolism, and signal transduction. The identification of three enzymes involved in S-adenosylmethionine (SAM) metabolism furthermore suggests that redox control is required to balance the metabolic fluxes of SAM between methyl-group transfer reactions and polyamine synthesis. To substantiate our data, the binding of the redoxins to S-adenosyl-L-homocysteine hydrolase and ornithine aminotransferase (OAT) were verified using BIAcore surface plasmon resonance. In enzymatic assays, Trx was furthermore shown to enhance the activity of OAT. Our approach led to the discovery of several putatively redox-regulated proteins, thereby contributing to our understanding of the redox interactome in malarial parasites." @default.
• W2073470794 created "2016-06-24" @default.
• W2073470794 creator A5028404931 @default.
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• W2073470794 date "2009-04-10" @default.
• W2073470794 modified "2023-10-11" @default.
• W2073470794 title "Identification of Proteins Targeted by the Thioredoxin Superfamily in Plasmodium falciparum" @default.
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• W2073470794 doi "https://doi.org/10.1371/journal.ppat.1000383" @default.
• W2073470794 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2660430" @default.
• W2073470794 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19360125" @default.
• W2073470794 hasPublicationYear "2009" @default.
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