Matches in SemOpenAlex for { <https://semopenalex.org/work/W2073490551> ?p ?o ?g. }
- W2073490551 endingPage "242" @default.
- W2073490551 startingPage "234" @default.
- W2073490551 abstract "The contribution of platelet‐activating factor (PAF) to platelet deposition and oedema formation induced by exogenous soluble mediators, zymosan particles and associated with a reversed passive Arthus (RPA) reaction in rabbit skin was investigated by use of a novel long‐acting PAF receptor antagonist, UK‐74,505. Oedema formation and platelet accumulation were simultaneously measured by i.v. injection of [ 125 I]‐albumin and 111 In‐labelled rabbit platelets. UK‐74,505 was either administered i.v. or used to pretreat radiolabelled platelets in vitro before their injection into recipient animals. Platelets pretreated with UK‐74,505 were also labelled with the fluorescent calcium indicator, Fura‐2, to assess their ex vivo reactivity to PAF at the end of the in vivo experiment. UK‐74,505 (0.5 mg kg −1 ), administered i.v., inhibited PAF‐induced oedema formation, but did not affect oedema induced by zymosan particles, bradykinin (BK), histamine, formyl‐methionyl‐leucyl‐phenylalanine (FMLP), zymosan‐activated plasma (ZAP, as a source of C5a des Arg), leukotriene B 4 (LTB 4 ) or interleukin‐8 (IL‐8). UK‐74,505, administered i.v. also suppressed the small platelet accumulation induced by exogenous PAF, but had no effect on accumulation induced by IL‐8 or ZAP. Although oedema induced by zymosan was not affected by i.v. UK‐74,505, zymosan‐induced platelet accumulation was significantly attenuated by the antagonist. The RPA reaction in rabbit skin was associated with marked oedema formation and platelet accumulation which were both inhibited by i.v. UK‐74,505. In vitro , UK‐74,505 inhibited aggregation and the increase in intracellular calcium concentration induced by PAF in rabbit washed platelets in a concentration‐dependent manner (IC 50 = 1.6 × 10 −8 m and 1.1 × 10 −8 m , respectively). Platelets pretreated with 10 −6 m UK‐74,505, and maintained at 37°C, were unresponsive to PAF, whilst responding normally to thrombin, for up to 4 h. In a second series of in vivo experiments, platelets were labelled with 111 In and loaded with Fura‐2. The platelets were then pretreated with 10 −6 m UK‐74,505, washed, and injected into recipient rabbits. These platelets, prepared from blood samples taken at the end of the in vivo experiments, exhibited an 80% reduction in their response to PAF as measured ex vivo with Fura‐2. However, in contrast to the effects of i.v. UK‐74,505, platelets pretreated with the antagonist did accumulate effectively in the RPA reaction, a significant reduction only being observed in responses at the lowest antibody dose. In addition, pretreatment of platelets had no effect on the small platelet accumulation induced by PAF. These results suggest that PAF is an important mediator of oedema formation and platelet accumulation in the RPA reaction in rabbit skin. However, they question the role of PAF receptors on platelets in this model. The results also indicate that PAF may be involved in platelet accumulation induced by zymosan in rabbit skin." @default.
- W2073490551 created "2016-06-24" @default.
- W2073490551 creator A5016151265 @default.
- W2073490551 creator A5022982229 @default.
- W2073490551 creator A5029036841 @default.
- W2073490551 creator A5029073857 @default.
- W2073490551 creator A5033052914 @default.
- W2073490551 date "1993-05-01" @default.
- W2073490551 modified "2023-10-17" @default.
- W2073490551 title "Role of platelet-activating factor (PAF) in platelet accumulation in rabbit skin: effect of the novel long-acting PAF antagonist, UK-74,505" @default.
- W2073490551 cites W1481337295 @default.
- W2073490551 cites W1543907558 @default.
- W2073490551 cites W1586968411 @default.
- W2073490551 cites W1596313010 @default.
- W2073490551 cites W1609051797 @default.
- W2073490551 cites W1972593824 @default.
- W2073490551 cites W1976934709 @default.
- W2073490551 cites W1987672928 @default.
- W2073490551 cites W1996024251 @default.
- W2073490551 cites W2004593352 @default.
- W2073490551 cites W2008390443 @default.
- W2073490551 cites W2009840801 @default.
- W2073490551 cites W2028034766 @default.
- W2073490551 cites W2029235746 @default.
- W2073490551 cites W2049835330 @default.
- W2073490551 cites W2052327108 @default.
- W2073490551 cites W2069421750 @default.
- W2073490551 cites W2070718618 @default.
- W2073490551 cites W2072517507 @default.
- W2073490551 cites W2090382275 @default.
- W2073490551 cites W2107568363 @default.
- W2073490551 cites W2141260882 @default.
- W2073490551 cites W2198762749 @default.
- W2073490551 cites W2336766625 @default.
- W2073490551 cites W2337850988 @default.
- W2073490551 cites W2411890782 @default.
- W2073490551 cites W2417276782 @default.
- W2073490551 cites W2472865104 @default.
- W2073490551 cites W4313308945 @default.
- W2073490551 doi "https://doi.org/10.1111/j.1476-5381.1993.tb13559.x" @default.
- W2073490551 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2175582" @default.
- W2073490551 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8495241" @default.
- W2073490551 hasPublicationYear "1993" @default.
- W2073490551 type Work @default.
- W2073490551 sameAs 2073490551 @default.
- W2073490551 citedByCount "19" @default.
- W2073490551 countsByYear W20734905512016 @default.
- W2073490551 crossrefType "journal-article" @default.
- W2073490551 hasAuthorship W2073490551A5016151265 @default.
- W2073490551 hasAuthorship W2073490551A5022982229 @default.
- W2073490551 hasAuthorship W2073490551A5029036841 @default.
- W2073490551 hasAuthorship W2073490551A5029073857 @default.
- W2073490551 hasAuthorship W2073490551A5033052914 @default.
- W2073490551 hasBestOaLocation W20734905512 @default.
- W2073490551 hasConcept C1122143 @default.
- W2073490551 hasConcept C126322002 @default.
- W2073490551 hasConcept C134018914 @default.
- W2073490551 hasConcept C150903083 @default.
- W2073490551 hasConcept C170493617 @default.
- W2073490551 hasConcept C185592680 @default.
- W2073490551 hasConcept C202751555 @default.
- W2073490551 hasConcept C207001950 @default.
- W2073490551 hasConcept C26291073 @default.
- W2073490551 hasConcept C2776685179 @default.
- W2073490551 hasConcept C2776885963 @default.
- W2073490551 hasConcept C2776914184 @default.
- W2073490551 hasConcept C2778122271 @default.
- W2073490551 hasConcept C2778870864 @default.
- W2073490551 hasConcept C2779020240 @default.
- W2073490551 hasConcept C2780955469 @default.
- W2073490551 hasConcept C55493867 @default.
- W2073490551 hasConcept C71924100 @default.
- W2073490551 hasConcept C86803240 @default.
- W2073490551 hasConcept C89560881 @default.
- W2073490551 hasConcept C98274493 @default.
- W2073490551 hasConceptScore W2073490551C1122143 @default.
- W2073490551 hasConceptScore W2073490551C126322002 @default.
- W2073490551 hasConceptScore W2073490551C134018914 @default.
- W2073490551 hasConceptScore W2073490551C150903083 @default.
- W2073490551 hasConceptScore W2073490551C170493617 @default.
- W2073490551 hasConceptScore W2073490551C185592680 @default.
- W2073490551 hasConceptScore W2073490551C202751555 @default.
- W2073490551 hasConceptScore W2073490551C207001950 @default.
- W2073490551 hasConceptScore W2073490551C26291073 @default.
- W2073490551 hasConceptScore W2073490551C2776685179 @default.
- W2073490551 hasConceptScore W2073490551C2776885963 @default.
- W2073490551 hasConceptScore W2073490551C2776914184 @default.
- W2073490551 hasConceptScore W2073490551C2778122271 @default.
- W2073490551 hasConceptScore W2073490551C2778870864 @default.
- W2073490551 hasConceptScore W2073490551C2779020240 @default.
- W2073490551 hasConceptScore W2073490551C2780955469 @default.
- W2073490551 hasConceptScore W2073490551C55493867 @default.
- W2073490551 hasConceptScore W2073490551C71924100 @default.
- W2073490551 hasConceptScore W2073490551C86803240 @default.
- W2073490551 hasConceptScore W2073490551C89560881 @default.
- W2073490551 hasConceptScore W2073490551C98274493 @default.
- W2073490551 hasIssue "1" @default.
- W2073490551 hasLocation W20734905511 @default.