Matches in SemOpenAlex for { <https://semopenalex.org/work/W2073497780> ?p ?o ?g. }
Showing items 1 to 80 of
80
with 100 items per page.
- W2073497780 endingPage "162" @default.
- W2073497780 startingPage "152" @default.
- W2073497780 abstract "The position-independent expression of trans-genes in target cells is an essential subject for determining effective gene therapies. The chicken β-globin insulator blocks the effects of regulatory sequences on transcriptional units at differential domains. We prepared a recombinant adeno-associated virus (rAAV) containing various combinations of the DNase I-hypersensitive site 2 (HS2), 3 (HS3), and 4 (HS4) core elements from the human β-globin locus control region (LCR), the human β-globin gene, and the herpes virus thymidine kinase promoter driven neomycin-resistant gene (neoR) (rHS432, rHS43, rHS42, rHS32, and rHS2), and also rAAV containing two copies of the 250-bp core sequence of the chicken β-globin insulator on both sides of the rHS2 (rIns/HS2/2Ins). After isolating neomycin-resistant mouse erythroleukemia (MEL) cells infected with each rAAV, we analyzed the rAAV genome by Southern blots and polymerase chain reaction (PCR), using primers specific for HS core elements and the human β-globin gene. All clones contained a single copy of the rAAV genome in the chromosome, however, some of them had a rearranged proviral genome. In five clones with a single unrearranged rAAV genome for each rAAV construct, we assayed the expression of the human b-globin gene relative to the endogenous mouse βmaj-globin gene, using quantitative reverse transcriptase (RT)-PCR. In clones infected with rHS432, the expression level of the human β-globin gene ranged from 51.6% to 765.6% of that in the mouse βmaj-globin gene. Likewise, in rHS43, the expression level ranged from 36.7% to 259.0%; in rHS42, from 47.8% to 207.0%; in rHS32, from 47.9% to 105.4%; and in rHS2, from 6.1% to 172.1%, indicating a high variability of expression level in clones infected with recombinant virus lacking the insulator. In contrast, in clones infected with rIns/HS2/Ins, the range of expression of the human β-globin gene ranged from 52.8% to 58.3% of that in the mouse βmaj-globin gene. These results indicate that the insulator functioned dramatically to reduce the variability of transgene expression due to the position effect. This insulator-rAAV vector system holds promise to provide a constant level of transgene expression for gene therapy, regardless of the insertion sites on the chromosome." @default.
- W2073497780 created "2016-06-24" @default.
- W2073497780 creator A5003879012 @default.
- W2073497780 creator A5007524794 @default.
- W2073497780 creator A5017966184 @default.
- W2073497780 creator A5032864679 @default.
- W2073497780 creator A5048838957 @default.
- W2073497780 creator A5051186559 @default.
- W2073497780 creator A5060925693 @default.
- W2073497780 date "1999-05-01" @default.
- W2073497780 modified "2023-10-18" @default.
- W2073497780 title "Position-independent human β-globin gene expression mediated by a recombinant adeno-associated virus vector carrying the chicken β-globin insulator" @default.
- W2073497780 doi "https://doi.org/10.1007/s100380050133" @default.
- W2073497780 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10319578" @default.
- W2073497780 hasPublicationYear "1999" @default.
- W2073497780 type Work @default.
- W2073497780 sameAs 2073497780 @default.
- W2073497780 citedByCount "30" @default.
- W2073497780 countsByYear W20734977802013 @default.
- W2073497780 countsByYear W20734977802014 @default.
- W2073497780 countsByYear W20734977802015 @default.
- W2073497780 crossrefType "journal-article" @default.
- W2073497780 hasAuthorship W2073497780A5003879012 @default.
- W2073497780 hasAuthorship W2073497780A5007524794 @default.
- W2073497780 hasAuthorship W2073497780A5017966184 @default.
- W2073497780 hasAuthorship W2073497780A5032864679 @default.
- W2073497780 hasAuthorship W2073497780A5048838957 @default.
- W2073497780 hasAuthorship W2073497780A5051186559 @default.
- W2073497780 hasAuthorship W2073497780A5060925693 @default.
- W2073497780 hasBestOaLocation W20734977801 @default.
- W2073497780 hasConcept C101762097 @default.
- W2073497780 hasConcept C104317684 @default.
- W2073497780 hasConcept C125996951 @default.
- W2073497780 hasConcept C133307689 @default.
- W2073497780 hasConcept C141231307 @default.
- W2073497780 hasConcept C150194340 @default.
- W2073497780 hasConcept C153911025 @default.
- W2073497780 hasConcept C159047783 @default.
- W2073497780 hasConcept C2778107364 @default.
- W2073497780 hasConcept C39981086 @default.
- W2073497780 hasConcept C40767141 @default.
- W2073497780 hasConcept C54355233 @default.
- W2073497780 hasConcept C86803240 @default.
- W2073497780 hasConcept C92087593 @default.
- W2073497780 hasConceptScore W2073497780C101762097 @default.
- W2073497780 hasConceptScore W2073497780C104317684 @default.
- W2073497780 hasConceptScore W2073497780C125996951 @default.
- W2073497780 hasConceptScore W2073497780C133307689 @default.
- W2073497780 hasConceptScore W2073497780C141231307 @default.
- W2073497780 hasConceptScore W2073497780C150194340 @default.
- W2073497780 hasConceptScore W2073497780C153911025 @default.
- W2073497780 hasConceptScore W2073497780C159047783 @default.
- W2073497780 hasConceptScore W2073497780C2778107364 @default.
- W2073497780 hasConceptScore W2073497780C39981086 @default.
- W2073497780 hasConceptScore W2073497780C40767141 @default.
- W2073497780 hasConceptScore W2073497780C54355233 @default.
- W2073497780 hasConceptScore W2073497780C86803240 @default.
- W2073497780 hasConceptScore W2073497780C92087593 @default.
- W2073497780 hasIssue "3" @default.
- W2073497780 hasLocation W20734977801 @default.
- W2073497780 hasLocation W20734977802 @default.
- W2073497780 hasOpenAccess W2073497780 @default.
- W2073497780 hasPrimaryLocation W20734977801 @default.
- W2073497780 hasRelatedWork W2044701705 @default.
- W2073497780 hasRelatedWork W2062919637 @default.
- W2073497780 hasRelatedWork W2073497780 @default.
- W2073497780 hasRelatedWork W2392849191 @default.
- W2073497780 hasRelatedWork W2402369711 @default.
- W2073497780 hasRelatedWork W2404327720 @default.
- W2073497780 hasRelatedWork W2411062448 @default.
- W2073497780 hasRelatedWork W2469148807 @default.
- W2073497780 hasRelatedWork W2571981199 @default.
- W2073497780 hasRelatedWork W4232129234 @default.
- W2073497780 hasVolume "44" @default.
- W2073497780 isParatext "false" @default.
- W2073497780 isRetracted "false" @default.
- W2073497780 magId "2073497780" @default.
- W2073497780 workType "article" @default.