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• W2073501085 abstract "For the purpose of investigating in detail the influence of chemical modification using 2-iminothiolane hydrochloride (2-IT) on keratin fibers, the structure of cross-sections at various depths of white human hair, treated with 2-IT and then oxidized, was directly analyzed without isolating the cuticle and cortex, using Raman spectroscopy. In particular, the β-sheet and/or random coil content (β/R) and the α-helix (α) content in human hair fibers were estimated by amide I band analysis. The SS band intensity, amide III (unordered) band intensity, and β/R content existing from the cuticle region to the center of cortex region of virgin white human hair remarkably increased by performing the chemical modification using 2-IT. On the other hand, not only the SS band intensity, but also SO band intensity existing throughout the cortex region of the bleached (damaged) white human hair increased by performing chemical modification using 2-IT. In particular, β/R content existing throughout the cortex region of the bleached white human hair decreased, while the skeletal CC stretch (α) band intensity at 935 cm−1 and the α content remarkably increased. This indicates a secondary structural change from the random coil form to the α-helix form in the proteins existing throughout the cortex region. From these experiments, we concluded that the formation of new disulfide (SS) groups resulting from chemical modification using 2-IT induced the secondary structural changes of proteins existing throughout the cortex region. © 2005 Wiley Periodicals, Inc. Biopolymers 79: 173–184, 2005 This article was originally published online as an accepted preprint. The “Published Online” date corresponds to the preprint version. You can request a copy of the preprint by emailing the Biopolymers editorial office at biopolymers@wiley.com" @default.
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• W2073501085 date "2005-01-01" @default.
• W2073501085 modified "2023-09-25" @default.
• W2073501085 title "Protein structural changes in keratin fibers induced by chemical modification using 2-iminothiolane hydrochloride: A Raman spectroscopic investigation" @default.
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• W2073501085 doi "https://doi.org/10.1002/bip.20329" @default.
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