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- W2073521211 abstract "N-Formylmethionyl transfer ribonucleic acid (fMettRNA) has been demonstrated in extracts from Escherichia coli [l] and subsequently from other sources, including Ehrlich ascites cells [2] , chloroplasts [3] and mitochondria of rat liver [4], yeast [4] and Hela cells [5] . It is not certain how extensively &let-tRNA is found in other bacterial species, since recent reviews [6-81 give no reports of it other than in E. coli. It has been suggested that intiation of protein biosynthesis, a role performed by fMet-tRNA in E. coli [9] , may involve alanine derivatives in Bacillus subtilis [6] . By comparison with several other bacterial species, two pseudomonads have been found to show an infrequent occurrence of methionine as the N-terminal residue of proteins [IO] . This might indicate that methionine residues are readily removed from the N-terminal position of proteins after synthesis, or that methionine derivatives participate only to a minor degree in the initiation process, in pseudomonas species. In view of this result and an observation [ 1 l] of two separable Met-tRNA synthetase activities in Pseudomonas aeruginosa, we have examined this organism to determine whether it contains fMet-tRNA. We find that fMet-tRNA and Met-tRNA are both present in growing cultures. After esterification of Ps. aeruginosa tRNA with radioactive methionine through the in vitro action of either of two partially purified Met-tRNA synthetases, as least two species of MettRNA are separable by chromatography on benzoylated DEAE cellulose (BDC) columns. The Met-tRNA species which elutes from the column more readily apparently corresponds to Met-tRNApet of E. coli in that it is" @default.
- W2073521211 created "2016-06-24" @default.
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- W2073521211 date "1970-05-11" @default.
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- W2073521211 title "N-Formylmethionyl transfer ribonucleic acid inPseudomonas aeruginosa" @default.
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- W2073521211 doi "https://doi.org/10.1016/0014-5793(70)80212-5" @default.
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