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- W2073605600 abstract "<h2>Abstract</h2> Thy-1 antigen expression in the rat thymic myoid cell line R615B2 (Thy-1(+)) and R613Ad (Thy-1(−)) was studied with a semi-quantitative immunohistochemical assay without disrupting the cell cultures. With this assay, the quantity of Thy-1 antigen on R615B2 cells was detected separately in the cytoplasm and on the cell surface by the use of appropriate fixatives such as chilled ethanol and methanol +0.3% H<sub>2</sub>O<sub>2</sub>. Extracellular Thy-1 antigen was also found in the culture supernatant of R615B2 cells. More than half of extracellular Thy-1 antigen remained in the supernatant even after 100 000 × <i>g</i> centrifugation. No form of Thy-1 antigen was detected at significant levels in R613Ad cells." @default.
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- W2073605600 date "1988-02-01" @default.
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- W2073605600 title "Semi-quantitative immunohistochemical studies on Thy-1 antigen expressed by thymic myoid cells" @default.
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- W2073605600 doi "https://doi.org/10.1016/0165-5728(88)90070-7" @default.
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