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• W2073634774 abstract "Using reverse transcription followed by PCR amplification (RT-PCR), we have identified multiple messenger RNAs encoding for the neuronal pore-forming Ca(2+) channel subunits alpha(1A) (P/Q channel), alpha(1B) (N channel), alpha(1D) (neuronal/endocrine L channel), alpha(1E) (R channel), alpha(1G-H) (T channel) and alpha(1S) (skeletal muscle L channel) in bovine chromaffin cells. mRNAs for the auxiliary beta(2), beta(3), beta(4), alpha(2)/delta and gamma(2) subunits were also identified. In agreement with these molecular data, perforated patch-clamp recordings of whole-cell Ca(2+) currents reveal the existence of functional R-type Ca(2+) channels in these cells that were previously undetected with other techniques. Our results provide a molecular frame for a much wider functional diversity of Ca(2+) channels in chromaffin cells than that previously established using pharmacological and electrophysiological approaches." @default.
• W2073634774 created "2016-06-24" @default.
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• W2073634774 date "2000-09-20" @default.
• W2073634774 modified "2023-10-16" @default.
• W2073634774 title "Greater diversity than previously thought of chromaffin cell Ca²⁺ channels, derived from mRNA identification studies" @default.
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• W2073634774 doi "https://doi.org/10.1016/s0014-5793(00)01984-0" @default.
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