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- W2073639342 abstract "Abstract Quantitative cytofluorometric measurements of total nucleic acids, DNA, RNA, total protein, histones, non-histone protein, protein-bound lysine and protein-bound arginine were made on nuclei in outer epidermal cells of the equatorial region ou white onion bulb leaf bases in two locations (0 to 5 mm and 16 to 20 mm) adjacent to wounded and Botrytis allii infected tissue. Wounding caused all nuclear indices measured to increase continuously over the 48 h study period. Exposure of the tissue to the ambient atmosphere caused slight increases over the same time. Inoculation caused all nuclear indices, except arginine, to increase during the first 24 h after inoculation. These increases were greater than those in non-wounded tissue exposed to the ambient atmosphere but less than those in tissue adjacent to wounds. At 48 h after inoculation, drastic degenerative changes were evident in all nuclear indices, especially in RNA and non-histone protein. We conclude that wounding caused host cells to change from a quiescent to an active state. Infection did not alter these changes until toxic fungal secretions, produced during establishment and spread into the host, caused cellular symptoms resembling senescence and death, and tissue symptoms in natural infections. This is the first report describing macromolecular changes occurring in nuclei of host cells affected by a facultative fungal pathogen." @default.
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- W2073639342 date "1982-09-01" @default.
- W2073639342 modified "2023-10-16" @default.
- W2073639342 title "Cytofluorometric study of onion epidermal nuclei in response to wounding and Botrytis allii infection" @default.
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- W2073639342 doi "https://doi.org/10.1016/0048-4059(82)90040-6" @default.
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