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• W2073648506 abstract "Ability to ferment arabinose is a variable characteristic in the species Vibrio parahaemolyticus. To study the genetic basis of this variability, the arabinose fermentation genes from an arabinose utilizing strain were cloned into the plasmid vector pBR322. Complementation studies employing plasmids deleted in segments of the cloned DNA established the presence of genes analogous to the araA, araB, araC, and araD genes of E. coli. The order of the ara genes in V. parahaemolyticus is different than that in Escherichia coli or Bacillus subtilis. Three radioactively labelled probes spanning the ara structural gene region were hybridized under stringent conditions with HindIII digested genomic DNA from arabinose positive and negative strains of V. parahaemolyticus and other Vibrio species. No DNA homologous to the probes was found in the arabinose negative V. parahaemolyticus strains indicating that absence rather than mutational inactivation of gene expression was responsible for the Ara- phenotype. Little or no hybridization of the ara probes to arabinose positive Vibrio fluvialis and Vibrio hollisae strains was detected." @default.
• W2073648506 created "2016-06-24" @default.
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• W2073648506 date "1992-08-01" @default.
• W2073648506 modified "2023-09-25" @default.
• W2073648506 title "Genetic Basis for Variability in Arabinose Fermentation in Vibrio parahaemolyticus" @default.
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• W2073648506 doi "https://doi.org/10.1016/s0723-2020(11)80216-x" @default.
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