Matches in SemOpenAlex for { <https://semopenalex.org/work/W2073669468> ?p ?o ?g. }
Showing items 1 to 93 of
93
with 100 items per page.
- W2073669468 endingPage "6117" @default.
- W2073669468 startingPage "6113" @default.
- W2073669468 abstract "A novelly developed tweezing-adsorptive bubble separation (ABS) method for the enrichment of metalloenzymes (laccase C and horseradish peroxidase) is introduced. The method is based on the chelation of the enzymes' active center and can also be applied for analysis. N-(2-acetamido)iminodiacetic acid served as a chelator and was synthesized with an octyl unit to become ADA-C8. Laccase was enriched 13.3-fold (66.31% recovery) and HPOX 17.8-fold (85.34%) without a significant loss of enzymatic activity. To prove that the entire enzyme is tweezed at the active center, ABS trials were done using ADA-C8 already complexed with Cu2+ and Fe3+. As only marginal enrichment occurred (ER laccase, 0.17; ER HPOX, 0.44), no chelating effect was concluded. It was determined how the chelation toward the active center was directed by applying other chelators such as EDTA, NTA, N,N-dimethylaminoglycine, oxalic acid, malonic acid, adipinic acid, and tripropylamine, which are similar in structure to ADA-C8. The results concluded that the chelation is 3-fold coordinated on the type 1 copper center of laccase, whereas that of HPOX only 1-fold at Fe3+ and additionally at the cationic amino acid arginine, which is also located at the active center. Tweezing-ABS has been proven to selectively and effectively enrich metalloenzymes." @default.
- W2073669468 created "2016-06-24" @default.
- W2073669468 creator A5019893279 @default.
- W2073669468 creator A5038690505 @default.
- W2073669468 creator A5043332345 @default.
- W2073669468 creator A5044700923 @default.
- W2073669468 creator A5047708536 @default.
- W2073669468 creator A5076700379 @default.
- W2073669468 date "2005-08-27" @default.
- W2073669468 modified "2023-09-26" @default.
- W2073669468 title "Tweezing-Adsorptive Bubble Separation. Analytical Method for the Selective and High Enrichment of Metalloenzymes" @default.
- W2073669468 cites W1982441025 @default.
- W2073669468 cites W2009743557 @default.
- W2073669468 cites W2012206960 @default.
- W2073669468 cites W2018101562 @default.
- W2073669468 cites W2032094105 @default.
- W2073669468 cites W2035376768 @default.
- W2073669468 cites W2041784590 @default.
- W2073669468 cites W2077717357 @default.
- W2073669468 cites W2081214123 @default.
- W2073669468 cites W2085501958 @default.
- W2073669468 cites W2121180772 @default.
- W2073669468 cites W2132305141 @default.
- W2073669468 cites W2156782854 @default.
- W2073669468 cites W2165898505 @default.
- W2073669468 cites W2921726565 @default.
- W2073669468 doi "https://doi.org/10.1021/ac050977s" @default.
- W2073669468 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/16194067" @default.
- W2073669468 hasPublicationYear "2005" @default.
- W2073669468 type Work @default.
- W2073669468 sameAs 2073669468 @default.
- W2073669468 citedByCount "14" @default.
- W2073669468 countsByYear W20736694682012 @default.
- W2073669468 countsByYear W20736694682017 @default.
- W2073669468 countsByYear W20736694682018 @default.
- W2073669468 countsByYear W20736694682019 @default.
- W2073669468 countsByYear W20736694682020 @default.
- W2073669468 countsByYear W20736694682022 @default.
- W2073669468 crossrefType "journal-article" @default.
- W2073669468 hasAuthorship W2073669468A5019893279 @default.
- W2073669468 hasAuthorship W2073669468A5038690505 @default.
- W2073669468 hasAuthorship W2073669468A5043332345 @default.
- W2073669468 hasAuthorship W2073669468A5044700923 @default.
- W2073669468 hasAuthorship W2073669468A5047708536 @default.
- W2073669468 hasAuthorship W2073669468A5076700379 @default.
- W2073669468 hasConcept C13965031 @default.
- W2073669468 hasConcept C178790620 @default.
- W2073669468 hasConcept C179104552 @default.
- W2073669468 hasConcept C181199279 @default.
- W2073669468 hasConcept C185592680 @default.
- W2073669468 hasConcept C197404232 @default.
- W2073669468 hasConcept C21951064 @default.
- W2073669468 hasConcept C2777676563 @default.
- W2073669468 hasConcept C2778861492 @default.
- W2073669468 hasConcept C2781137352 @default.
- W2073669468 hasConcept C2781346138 @default.
- W2073669468 hasConcept C43617362 @default.
- W2073669468 hasConcept C76935637 @default.
- W2073669468 hasConceptScore W2073669468C13965031 @default.
- W2073669468 hasConceptScore W2073669468C178790620 @default.
- W2073669468 hasConceptScore W2073669468C179104552 @default.
- W2073669468 hasConceptScore W2073669468C181199279 @default.
- W2073669468 hasConceptScore W2073669468C185592680 @default.
- W2073669468 hasConceptScore W2073669468C197404232 @default.
- W2073669468 hasConceptScore W2073669468C21951064 @default.
- W2073669468 hasConceptScore W2073669468C2777676563 @default.
- W2073669468 hasConceptScore W2073669468C2778861492 @default.
- W2073669468 hasConceptScore W2073669468C2781137352 @default.
- W2073669468 hasConceptScore W2073669468C2781346138 @default.
- W2073669468 hasConceptScore W2073669468C43617362 @default.
- W2073669468 hasConceptScore W2073669468C76935637 @default.
- W2073669468 hasIssue "19" @default.
- W2073669468 hasLocation W20736694681 @default.
- W2073669468 hasLocation W20736694682 @default.
- W2073669468 hasOpenAccess W2073669468 @default.
- W2073669468 hasPrimaryLocation W20736694681 @default.
- W2073669468 hasRelatedWork W1501461774 @default.
- W2073669468 hasRelatedWork W2006242064 @default.
- W2073669468 hasRelatedWork W2059181494 @default.
- W2073669468 hasRelatedWork W2070543174 @default.
- W2073669468 hasRelatedWork W2073669468 @default.
- W2073669468 hasRelatedWork W2386094873 @default.
- W2073669468 hasRelatedWork W2392227328 @default.
- W2073669468 hasRelatedWork W2404169606 @default.
- W2073669468 hasRelatedWork W2942553627 @default.
- W2073669468 hasRelatedWork W2949277261 @default.
- W2073669468 hasVolume "77" @default.
- W2073669468 isParatext "false" @default.
- W2073669468 isRetracted "false" @default.
- W2073669468 magId "2073669468" @default.
- W2073669468 workType "article" @default.