Matches in SemOpenAlex for { <https://semopenalex.org/work/W2073727424> ?p ?o ?g. }
Showing items 1 to 94 of
94
with 100 items per page.
- W2073727424 endingPage "2522" @default.
- W2073727424 startingPage "2518" @default.
- W2073727424 abstract "Overexpression of Bcl-2, a typical anti-apoptotic protein, is one of the most effective means to maintain mitochondria integrity in recombinant CHO (rCHO) cell culture treated with sodium butyrate (NaBu). NaBu is known as a typical specific productivity-enhancing factor and also a well-known apoptosis inducer. Bcl-2 is distributed to and functions in multiple intracellular organelles such as the nucleus, mitochondria, and endoplasmic reticulum (ER). To evaluate the effect of organelle-specific overexpression of Bcl-2 on NaBu-induced apoptosis in rCHO cells, Bcl-2 expression was restricted to the mitochondria or to the ER either by employing a mitochondrial insertion sequence of ActA or by insertion of an ER-specific sequence of cytochrome b5 to their respective sequences. The rCHO cell lines overexpressing wild-type Bcl-2 (WT-Bcl-2), mitochondrial Bcl-2 (MT-Bcl-2), and ER-targeted Bcl-2 (ER-Bcl-2) were established. Overexpression of WT-Bcl-2, MT-Bcl-2, and ER-Bcl-2 could increase cell viability and decrease LDH release under NaBu-treated conditions. Additionally, overexpression of WT-Bcl-2, MT-Bcl-2, and ER-Bcl-2 could suppress NaBu-induced apoptosis, as demonstrated by a DNA fragmentation assay. A mitochondrial membrane potential assay revealed that ER-Bcl-2 overexpression can maintain the mitochondrial membrane integrity without being affected by MT-Bcl-2 overexpression, indicating that the role of ER should be considered in alleviating NaBu-induced apoptosis by a genetic modulation strategy. Taken together, it was found that restricted Bcl-2 overexpression at the ER can inhibit the NaBu-induced apoptosis by maintaining mitochondria integrity in rCHO cells." @default.
- W2073727424 created "2016-06-24" @default.
- W2073727424 creator A5003659661 @default.
- W2073727424 creator A5007258981 @default.
- W2073727424 creator A5014571955 @default.
- W2073727424 creator A5025205181 @default.
- W2073727424 creator A5034459594 @default.
- W2073727424 creator A5043492149 @default.
- W2073727424 creator A5052262819 @default.
- W2073727424 creator A5085507274 @default.
- W2073727424 date "2012-12-01" @default.
- W2073727424 modified "2023-09-25" @default.
- W2073727424 title "Effect of mitochondrial and ER-targeted Bcl-2 overexpression on apoptosis in recombinant Chinese hamster ovary cells treated with sodium butyrate" @default.
- W2073727424 cites W1933007466 @default.
- W2073727424 cites W1972336737 @default.
- W2073727424 cites W1974333522 @default.
- W2073727424 cites W2013065884 @default.
- W2073727424 cites W2045074898 @default.
- W2073727424 cites W2063379481 @default.
- W2073727424 cites W2067985632 @default.
- W2073727424 cites W2080091995 @default.
- W2073727424 cites W2089883223 @default.
- W2073727424 cites W2094389211 @default.
- W2073727424 cites W2098291790 @default.
- W2073727424 cites W2123793943 @default.
- W2073727424 cites W2142309981 @default.
- W2073727424 cites W2145167446 @default.
- W2073727424 cites W2158971210 @default.
- W2073727424 cites W2166474895 @default.
- W2073727424 cites W2318252390 @default.
- W2073727424 cites W2331099556 @default.
- W2073727424 cites W53848852 @default.
- W2073727424 doi "https://doi.org/10.1016/j.procbio.2012.06.021" @default.
- W2073727424 hasPublicationYear "2012" @default.
- W2073727424 type Work @default.
- W2073727424 sameAs 2073727424 @default.
- W2073727424 citedByCount "0" @default.
- W2073727424 crossrefType "journal-article" @default.
- W2073727424 hasAuthorship W2073727424A5003659661 @default.
- W2073727424 hasAuthorship W2073727424A5007258981 @default.
- W2073727424 hasAuthorship W2073727424A5014571955 @default.
- W2073727424 hasAuthorship W2073727424A5025205181 @default.
- W2073727424 hasAuthorship W2073727424A5034459594 @default.
- W2073727424 hasAuthorship W2073727424A5043492149 @default.
- W2073727424 hasAuthorship W2073727424A5052262819 @default.
- W2073727424 hasAuthorship W2073727424A5085507274 @default.
- W2073727424 hasConcept C153911025 @default.
- W2073727424 hasConcept C158617107 @default.
- W2073727424 hasConcept C175656101 @default.
- W2073727424 hasConcept C185592680 @default.
- W2073727424 hasConcept C190283241 @default.
- W2073727424 hasConcept C2778942008 @default.
- W2073727424 hasConcept C28859421 @default.
- W2073727424 hasConcept C29311851 @default.
- W2073727424 hasConcept C54355233 @default.
- W2073727424 hasConcept C55493867 @default.
- W2073727424 hasConcept C81885089 @default.
- W2073727424 hasConcept C86803240 @default.
- W2073727424 hasConcept C95444343 @default.
- W2073727424 hasConceptScore W2073727424C153911025 @default.
- W2073727424 hasConceptScore W2073727424C158617107 @default.
- W2073727424 hasConceptScore W2073727424C175656101 @default.
- W2073727424 hasConceptScore W2073727424C185592680 @default.
- W2073727424 hasConceptScore W2073727424C190283241 @default.
- W2073727424 hasConceptScore W2073727424C2778942008 @default.
- W2073727424 hasConceptScore W2073727424C28859421 @default.
- W2073727424 hasConceptScore W2073727424C29311851 @default.
- W2073727424 hasConceptScore W2073727424C54355233 @default.
- W2073727424 hasConceptScore W2073727424C55493867 @default.
- W2073727424 hasConceptScore W2073727424C81885089 @default.
- W2073727424 hasConceptScore W2073727424C86803240 @default.
- W2073727424 hasConceptScore W2073727424C95444343 @default.
- W2073727424 hasFunder F4320322349 @default.
- W2073727424 hasIssue "12" @default.
- W2073727424 hasLocation W20737274241 @default.
- W2073727424 hasOpenAccess W2073727424 @default.
- W2073727424 hasPrimaryLocation W20737274241 @default.
- W2073727424 hasRelatedWork W2032749643 @default.
- W2073727424 hasRelatedWork W2038611542 @default.
- W2073727424 hasRelatedWork W2054490148 @default.
- W2073727424 hasRelatedWork W2124482434 @default.
- W2073727424 hasRelatedWork W2348771880 @default.
- W2073727424 hasRelatedWork W2378662303 @default.
- W2073727424 hasRelatedWork W2383263084 @default.
- W2073727424 hasRelatedWork W2795861779 @default.
- W2073727424 hasRelatedWork W3046665064 @default.
- W2073727424 hasRelatedWork W3138176027 @default.
- W2073727424 hasVolume "47" @default.
- W2073727424 isParatext "false" @default.
- W2073727424 isRetracted "false" @default.
- W2073727424 magId "2073727424" @default.
- W2073727424 workType "article" @default.