Matches in SemOpenAlex for { <https://semopenalex.org/work/W2073742601> ?p ?o ?g. }
- W2073742601 abstract "TAL-effector nucleases (TALENs) are attractive tools for sequence-specific genome modifications, but their delivery still remains problematic. It is well known that the presence of multiple sequence repeats in TALEN genes hampers the use of lentiviral vectors. We report that lentiviral vectors readily package full-length vector mRNAs encoding TALENs, but recombination during reverse transcription prevents successful delivery. We reasoned that preventing reverse transcription of lentiviral-vector RNA would allow transfer of TALENs as mRNA. We demonstrate that lentiviral particles containing genetically inactivated reverse transcriptase (RT) mediated efficient transduction of cultured cells and supported transient transgene expression. For proof-of-principle, we transferred CCR5- and TCR-specific TALEN pairs for efficient targeted genome editing and abrogated expression for each of the receptor proteins in different cell lines. Combining the high specificity of TALENs with efficient lentiviral gene delivery should advance genome editing in vitro and potentially in vivo, and RT-deficient lentiviral vectors may be useful for transient expression of various other genes-of-interest." @default.
- W2073742601 created "2016-06-24" @default.
- W2073742601 creator A5044799435 @default.
- W2073742601 creator A5052933885 @default.
- W2073742601 creator A5057568404 @default.
- W2073742601 creator A5064943163 @default.
- W2073742601 creator A5067320487 @default.
- W2073742601 creator A5072950033 @default.
- W2073742601 creator A5089375913 @default.
- W2073742601 date "2014-09-18" @default.
- W2073742601 modified "2023-10-12" @default.
- W2073742601 title "Novel lentiviral vectors with mutated reverse transcriptase for mRNA delivery of TALE nucleases" @default.
- W2073742601 cites W118864685 @default.
- W2073742601 cites W1673293585 @default.
- W2073742601 cites W1681143022 @default.
- W2073742601 cites W1769569140 @default.
- W2073742601 cites W1971732348 @default.
- W2073742601 cites W1974178985 @default.
- W2073742601 cites W1979657319 @default.
- W2073742601 cites W1981686597 @default.
- W2073742601 cites W1982969769 @default.
- W2073742601 cites W1983468609 @default.
- W2073742601 cites W1993510145 @default.
- W2073742601 cites W1996625697 @default.
- W2073742601 cites W1998707281 @default.
- W2073742601 cites W2008033906 @default.
- W2073742601 cites W2008553597 @default.
- W2073742601 cites W2015231167 @default.
- W2073742601 cites W2016042182 @default.
- W2073742601 cites W2023421219 @default.
- W2073742601 cites W2027051533 @default.
- W2073742601 cites W2029430860 @default.
- W2073742601 cites W2031780150 @default.
- W2073742601 cites W2039705224 @default.
- W2073742601 cites W2055125531 @default.
- W2073742601 cites W2061184966 @default.
- W2073742601 cites W2064815984 @default.
- W2073742601 cites W2073973240 @default.
- W2073742601 cites W2075706751 @default.
- W2073742601 cites W2077659966 @default.
- W2073742601 cites W2080366675 @default.
- W2073742601 cites W2083985858 @default.
- W2073742601 cites W2090787765 @default.
- W2073742601 cites W2095433332 @default.
- W2073742601 cites W2097606337 @default.
- W2073742601 cites W2100261765 @default.
- W2073742601 cites W2101754090 @default.
- W2073742601 cites W2110260408 @default.
- W2073742601 cites W2116565356 @default.
- W2073742601 cites W2125524975 @default.
- W2073742601 cites W2126189632 @default.
- W2073742601 cites W2128377922 @default.
- W2073742601 cites W2132401721 @default.
- W2073742601 cites W2132607153 @default.
- W2073742601 cites W2132919618 @default.
- W2073742601 cites W2133776494 @default.
- W2073742601 cites W2137440275 @default.
- W2073742601 cites W2138231299 @default.
- W2073742601 cites W2140658947 @default.
- W2073742601 cites W2154853638 @default.
- W2073742601 cites W2163366601 @default.
- W2073742601 cites W2164316881 @default.
- W2073742601 cites W2167845957 @default.
- W2073742601 cites W2171234781 @default.
- W2073742601 doi "https://doi.org/10.1038/srep06409" @default.
- W2073742601 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4166709" @default.
- W2073742601 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25230987" @default.
- W2073742601 hasPublicationYear "2014" @default.
- W2073742601 type Work @default.
- W2073742601 sameAs 2073742601 @default.
- W2073742601 citedByCount "51" @default.
- W2073742601 countsByYear W20737426012015 @default.
- W2073742601 countsByYear W20737426012016 @default.
- W2073742601 countsByYear W20737426012017 @default.
- W2073742601 countsByYear W20737426012018 @default.
- W2073742601 countsByYear W20737426012019 @default.
- W2073742601 countsByYear W20737426012020 @default.
- W2073742601 countsByYear W20737426012021 @default.
- W2073742601 countsByYear W20737426012022 @default.
- W2073742601 countsByYear W20737426012023 @default.
- W2073742601 crossrefType "journal-article" @default.
- W2073742601 hasAuthorship W2073742601A5044799435 @default.
- W2073742601 hasAuthorship W2073742601A5052933885 @default.
- W2073742601 hasAuthorship W2073742601A5057568404 @default.
- W2073742601 hasAuthorship W2073742601A5064943163 @default.
- W2073742601 hasAuthorship W2073742601A5067320487 @default.
- W2073742601 hasAuthorship W2073742601A5072950033 @default.
- W2073742601 hasAuthorship W2073742601A5089375913 @default.
- W2073742601 hasBestOaLocation W20737426011 @default.
- W2073742601 hasConcept C102230213 @default.
- W2073742601 hasConcept C104317684 @default.
- W2073742601 hasConcept C111829913 @default.
- W2073742601 hasConcept C141231307 @default.
- W2073742601 hasConcept C144501496 @default.
- W2073742601 hasConcept C15152581 @default.
- W2073742601 hasConcept C156719811 @default.
- W2073742601 hasConcept C31909778 @default.
- W2073742601 hasConcept C32470452 @default.
- W2073742601 hasConcept C40767141 @default.
- W2073742601 hasConcept C51785407 @default.