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- W2073753755 abstract "An enzyme electrode with a chemically-amplified response forl-lactate is constructed from an oxygen electrode and a layer containing immobilized lactate oxidase, to oxidizel-lactate, and lactate dehydrogenase, to regenerate thel-lactate. Regeneration enables oxygen to be consumed beyond the stoichiometric limitation, which results in an electrode response amplified 2–250 times according to the variation in the layer properties such as the Vmax and Km values of the immobilized enzymes and the thickness of the layer. The detection limit is as low as 5 × 10−9 M. An equation is derived to relate the rate of oxygen consumption in the layer to the experimental parameters; the equation successfully explains the experimental results." @default.
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- W2073753755 date "1985-01-01" @default.
- W2073753755 modified "2023-10-14" @default.
- W2073753755 title "An enzyme electrode forl-lactate with a chemically-amplified response" @default.
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- W2073753755 doi "https://doi.org/10.1016/s0003-2670(00)82947-5" @default.
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