FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2073756937> ?p ?o ?g. }

• W2073756937 endingPage "e1000973" @default.
• W2073756937 startingPage "e1000973" @default.
• W2073756937 abstract "In budding yeast, an HO endonuclease-inducible double-strand break (DSB) is efficiently repaired by several homologous recombination (HR) pathways. In contrast to gene conversion (GC), where both ends of the DSB can recombine with the same template, break-induced replication (BIR) occurs when only the centromere-proximal end of the DSB can locate homologous sequences. Whereas GC results in a small patch of new DNA synthesis, BIR leads to a nonreciprocal translocation. The requirements for completing BIR are significantly different from those of GC, but both processes require 5' to 3' resection of DSB ends to create single-stranded DNA that leads to formation of a Rad51 filament required to initiate HR. Resection proceeds by two pathways dependent on Exo1 or the BLM homolog, Sgs1. We report that Exo1 and Sgs1 each inhibit BIR but have little effect on GC, while overexpression of either protein severely inhibits BIR. In contrast, overexpression of Rad51 markedly increases the efficiency of BIR, again with little effect on GC. In sgs1Delta exo1Delta strains, where there is little 5' to 3' resection, the level of BIR is not different from either single mutant; surprisingly, there is a two-fold increase in cell viability after HO induction whereby 40% of all cells survive by formation of a new telomere within a few kb of the site of DNA cleavage. De novo telomere addition is rare in wild-type, sgs1Delta, or exo1Delta cells. In sgs1Delta exo1Delta, repair by GC is severely inhibited, but cell viability remains high because of new telomere formation. These data suggest that the extensive 5' to 3' resection that occurs before the initiation of new DNA synthesis in BIR may prevent efficient maintenance of a Rad51 filament near the DSB end. The severe constraint on 5' to 3' resection, which also abrogates activation of the Mec1-dependent DNA damage checkpoint, permits an unprecedented level of new telomere addition." @default.
• W2073756937 created "2016-06-24" @default.
• W2073756937 creator A5000513649 @default.
• W2073756937 creator A5004152549 @default.
• W2073756937 creator A5048113506 @default.
• W2073756937 creator A5053069378 @default.
• W2073756937 creator A5090373311 @default.
• W2073756937 date "2010-05-27" @default.
• W2073756937 modified "2023-10-14" @default.
• W2073756937 title "Sgs1 and Exo1 Redundantly Inhibit Break-Induced Replication and De Novo Telomere Addition at Broken Chromosome Ends" @default.
• W2073756937 cites W1480404031 @default.
• W2073756937 cites W1565890429 @default.
• W2073756937 cites W1606954381 @default.
• W2073756937 cites W1861951729 @default.
• W2073756937 cites W1905978644 @default.
• W2073756937 cites W1967779036 @default.
• W2073756937 cites W1978054036 @default.
• W2073756937 cites W1980623630 @default.
• W2073756937 cites W1984693763 @default.
• W2073756937 cites W1989136612 @default.
• W2073756937 cites W1993026474 @default.
• W2073756937 cites W2001680897 @default.
• W2073756937 cites W2003236929 @default.
• W2073756937 cites W2004099247 @default.
• W2073756937 cites W2009825306 @default.
• W2073756937 cites W2011792017 @default.
• W2073756937 cites W2011917032 @default.
• W2073756937 cites W2016357039 @default.
• W2073756937 cites W2024414745 @default.
• W2073756937 cites W2024442567 @default.
• W2073756937 cites W2025888720 @default.
• W2073756937 cites W2041065032 @default.
• W2073756937 cites W2052202220 @default.
• W2073756937 cites W2052547134 @default.
• W2073756937 cites W2055425310 @default.
• W2073756937 cites W2055667487 @default.
• W2073756937 cites W2057072455 @default.
• W2073756937 cites W2061060103 @default.
• W2073756937 cites W2062185406 @default.
• W2073756937 cites W2064728219 @default.
• W2073756937 cites W2065369524 @default.
• W2073756937 cites W2065612395 @default.
• W2073756937 cites W2066995090 @default.
• W2073756937 cites W2069218295 @default.
• W2073756937 cites W2069839388 @default.
• W2073756937 cites W2079027867 @default.
• W2073756937 cites W2082447947 @default.
• W2073756937 cites W2083980111 @default.
• W2073756937 cites W2094020226 @default.
• W2073756937 cites W2094981589 @default.
• W2073756937 cites W2095701756 @default.
• W2073756937 cites W2095729921 @default.
• W2073756937 cites W2101659216 @default.
• W2073756937 cites W2102470371 @default.
• W2073756937 cites W2109529827 @default.
• W2073756937 cites W2116471216 @default.
• W2073756937 cites W2123235480 @default.
• W2073756937 cites W2126492332 @default.
• W2073756937 cites W2126552646 @default.
• W2073756937 cites W2127073167 @default.
• W2073756937 cites W2127663205 @default.
• W2073756937 cites W2129225238 @default.
• W2073756937 cites W2131666315 @default.
• W2073756937 cites W2133491083 @default.
• W2073756937 cites W2135158692 @default.
• W2073756937 cites W2136367213 @default.
• W2073756937 cites W2136597072 @default.
• W2073756937 cites W2138122869 @default.
• W2073756937 cites W2140454994 @default.
• W2073756937 cites W2144550200 @default.
• W2073756937 cites W2146454701 @default.
• W2073756937 cites W2147344322 @default.
• W2073756937 cites W2153314555 @default.
• W2073756937 cites W2157434667 @default.
• W2073756937 cites W2158151897 @default.
• W2073756937 cites W2160005490 @default.
• W2073756937 cites W2162378349 @default.
• W2073756937 cites W2169333620 @default.
• W2073756937 cites W2169741450 @default.
• W2073756937 cites W4243056507 @default.
• W2073756937 doi "https://doi.org/10.1371/journal.pgen.1000973" @default.
• W2073756937 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2877739" @default.
• W2073756937 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/20523895" @default.
• W2073756937 hasPublicationYear "2010" @default.
• W2073756937 type Work @default.
• W2073756937 sameAs 2073756937 @default.
• W2073756937 citedByCount "80" @default.
• W2073756937 countsByYear W20737569372012 @default.
• W2073756937 countsByYear W20737569372013 @default.
• W2073756937 countsByYear W20737569372014 @default.
• W2073756937 countsByYear W20737569372015 @default.
• W2073756937 countsByYear W20737569372016 @default.
• W2073756937 countsByYear W20737569372017 @default.
• W2073756937 countsByYear W20737569372018 @default.
• W2073756937 countsByYear W20737569372019 @default.
• W2073756937 countsByYear W20737569372020 @default.
• W2073756937 countsByYear W20737569372021 @default.
• W2073756937 countsByYear W20737569372022 @default.

• next