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- W2073770021 abstract "The essential amino acid composition of plant foodstuffs is a very important nutritional property, especially in the developing countries where people often depend heavily on plant proteins from a single source. In these situations, it would be highly beneficial to ‘engineer’ the plant to produce proteins with a balanced essential amino acid content. Recombinant DNA technology and Agrobacterium-based vector systems offer a novel approach to modify plant proteins and raise the possibility of increasing their nutritive value. Methods are described for the inoculation of in vitro potato plants with Agrobacterium rhizogenes containing, within the Ri plasmid, a synthetic gene sequence coding for a protein high in essential amino acids. This synthetic gene fragment (HEAAE-DNA), 292 basepairs in length, codes for a protein composed of about 80% essential amino acids. Plants were regenerated from the Agrobacterium-induced hairy roots and we present data that these transformed plants contain the synthetic gene insert. Furthermore, our evidence shows that this novel gene was transcribed and translated within the transformed plant. Tubers from regenerated potato plants were subjected to analysis for introduction and expression of this gene. Integration of the gene into the plant genome and its expression into mRNAs and HEAAE-proteins have been demonstrated using Southern, northern and western analysis." @default.
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- W2073770021 date "1989-01-01" @default.
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- W2073770021 title "Expression of a synthetic gene for improved protein quality in transformed potato plants" @default.
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- W2073770021 doi "https://doi.org/10.1016/0168-9452(89)90156-8" @default.
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