FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2073776364> ?p ?o ?g. }

• W2073776364 endingPage "15548" @default.
• W2073776364 startingPage "15536" @default.
• W2073776364 abstract "S-Adenosylmethionine (AdoMet)-based methylation is integral to metabolism and signaling. AdoMet-dependent methyltransferases belong to multiple distinct classes and share a catalytic mechanism that arose through convergent evolution; however, fundamental determinants underlying this shared methyl transfer mechanism remain undefined. A survey of high-resolution crystal structures reveals that unconventional carbon–oxygen (CH···O) hydrogen bonds coordinate the AdoMet methyl group in different methyltransferases irrespective of their class, active site structure, or cofactor binding conformation. Corroborating these observations, quantum chemistry calculations demonstrate that these charged interactions formed by the AdoMet sulfonium cation are stronger than typical CH···O hydrogen bonds. Biochemical and structural studies using a model lysine methyltransferase and an active site mutant that abolishes CH···O hydrogen bonding to AdoMet illustrate that these interactions are important for high-affinity AdoMet binding and transition-state stabilization. Further, crystallographic and NMR dynamics experiments of the wild-type enzyme demonstrate that the CH···O hydrogen bonds constrain the motion of the AdoMet methyl group, potentially facilitating its alignment during catalysis. Collectively, the experimental findings with the model methyltransferase and structural survey imply that methyl CH···O hydrogen bonding represents a convergent evolutionary feature of AdoMet-dependent methyltransferases, mediating a universal mechanism for methyl transfer." @default.
• W2073776364 created "2016-06-24" @default.
• W2073776364 creator A5023133343 @default.
• W2073776364 creator A5030497987 @default.
• W2073776364 creator A5034049456 @default.
• W2073776364 creator A5035373472 @default.
• W2073776364 creator A5042792273 @default.
• W2073776364 creator A5044356587 @default.
• W2073776364 creator A5070609079 @default.
• W2073776364 creator A5070956130 @default.
• W2073776364 creator A5085422555 @default.
• W2073776364 creator A5091496526 @default.
• W2073776364 date "2013-10-07" @default.
• W2073776364 modified "2023-10-18" @default.
• W2073776364 title "Conservation and Functional Importance of Carbon–Oxygen Hydrogen Bonding in AdoMet-Dependent Methyltransferases" @default.
• W2073776364 cites W1516989687 @default.
• W2073776364 cites W1539796472 @default.
• W2073776364 cites W1551351981 @default.
• W2073776364 cites W1568187154 @default.
• W2073776364 cites W1673322472 @default.
• W2073776364 cites W1964286295 @default.
• W2073776364 cites W1971317954 @default.
• W2073776364 cites W1973056448 @default.
• W2073776364 cites W1973059973 @default.
• W2073776364 cites W1974802001 @default.
• W2073776364 cites W1976088117 @default.
• W2073776364 cites W1976391576 @default.
• W2073776364 cites W1979546920 @default.
• W2073776364 cites W1985152916 @default.
• W2073776364 cites W1987520312 @default.
• W2073776364 cites W1991941799 @default.
• W2073776364 cites W1995017064 @default.
• W2073776364 cites W1995535745 @default.
• W2073776364 cites W1998202852 @default.
• W2073776364 cites W1998391934 @default.
• W2073776364 cites W2001014236 @default.
• W2073776364 cites W2008388143 @default.
• W2073776364 cites W2009858080 @default.
• W2073776364 cites W2011104528 @default.
• W2073776364 cites W2011778857 @default.
• W2073776364 cites W2016045733 @default.
• W2073776364 cites W2018860787 @default.
• W2073776364 cites W2023272972 @default.
• W2073776364 cites W2026487543 @default.
• W2073776364 cites W2031502544 @default.
• W2073776364 cites W2033542024 @default.
• W2073776364 cites W2034156000 @default.
• W2073776364 cites W2035422514 @default.
• W2073776364 cites W2039020750 @default.
• W2073776364 cites W2039735251 @default.
• W2073776364 cites W2040381937 @default.
• W2073776364 cites W2042837962 @default.
• W2073776364 cites W2044125074 @default.
• W2073776364 cites W2048977450 @default.
• W2073776364 cites W2052087397 @default.
• W2073776364 cites W2055475531 @default.
• W2073776364 cites W2057867172 @default.
• W2073776364 cites W2059483202 @default.
• W2073776364 cites W2060043202 @default.
• W2073776364 cites W2066481443 @default.
• W2073776364 cites W2069893150 @default.
• W2073776364 cites W2070186920 @default.
• W2073776364 cites W2071788088 @default.
• W2073776364 cites W2072052008 @default.
• W2073776364 cites W2073924679 @default.
• W2073776364 cites W2074164587 @default.
• W2073776364 cites W2076387125 @default.
• W2073776364 cites W2077451095 @default.
• W2073776364 cites W2078652488 @default.
• W2073776364 cites W2079017121 @default.
• W2073776364 cites W2079482427 @default.
• W2073776364 cites W2079487727 @default.
• W2073776364 cites W2080972330 @default.
• W2073776364 cites W2080983914 @default.
• W2073776364 cites W2086176226 @default.
• W2073776364 cites W2094340803 @default.
• W2073776364 cites W2100071542 @default.
• W2073776364 cites W2102041979 @default.
• W2073776364 cites W2102577133 @default.
• W2073776364 cites W2111131931 @default.
• W2073776364 cites W2116514934 @default.
• W2073776364 cites W2119489910 @default.
• W2073776364 cites W2123914984 @default.
• W2073776364 cites W2124026197 @default.
• W2073776364 cites W2131639373 @default.
• W2073776364 cites W2132005991 @default.
• W2073776364 cites W2132629607 @default.
• W2073776364 cites W2133784816 @default.
• W2073776364 cites W2138870170 @default.
• W2073776364 cites W2148941593 @default.
• W2073776364 cites W2154714625 @default.
• W2073776364 cites W2166346311 @default.
• W2073776364 cites W2169262306 @default.
• W2073776364 cites W2169821755 @default.
• W2073776364 cites W2231749124 @default.
• W2073776364 cites W2313123091 @default.
• W2073776364 cites W2320540421 @default.
• W2073776364 cites W2327855492 @default.

• next