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- W2073819975 abstract "Rod outer segment disk membranes are isolated from dark-adapted frozen bovine retinae, osmotically swollen into spherical vesicles, and their photopigment content is determined. The size and shape of the vesicles are deduced from elastic and quasielastic light scatterings, the vesicle number density by viscometry, and the photopigment concentration by optical absorbance and by total protein assays of Lowry and Bradford. We find that rhodopsin constitutes about 95% of the membrane proteins and there are about 7 × 104 rhodopsin/vesicle. Further the vesicle surface area per rhodopsin is about 40 nm2. This leads to a rhodopsin nearest-neighbor distance of 5.5 nm on the vesicle surface which is in good agreement with results based on microspectrophotometry and X-ray diffraction." @default.
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- W2073819975 date "1981-06-01" @default.
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- W2073819975 title "Photopigment content of isolated bovine disk membrane vesicles" @default.
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- W2073819975 doi "https://doi.org/10.1016/0003-2697(81)90455-3" @default.
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