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• W2073836386 abstract "To determine the expression of SMAD transcripts in human granulosa cells. Luteinized mural granulosa cells were harvested from forty women undergoing oocyte retrieval, and RNAs were isolated. SMAD expression levels were determined by polymerase chain reaction (PCR) and quantitative real-time PCR (q-RTPCR). SMAD1-7 and 9 are expressed in human granulosa cells, with SMAD2, 3 and 4 showing the highest expression levels. Peak estradiol (E2) levels correlated with the number of oocytes retrieved during IVF. Oocyte number showed no correlation with SMAD expression levels or ratios. Fertilization rates also did not correlate with the expression levels of individual SMADs, but did correlate with higher SMAD4:SMAD3 ratios (p = 0.0062) and trended with SMAD4:SMAD2 (p = 0.0698). SMAD transcripts are differently expressed in human granulosa cells, where they may mediate TGF-beta superfamily signaling during folliculogenesis and ovulation. Further, the relative expression ratios of SMAD2, 3 and 4 may differentially affect fertilization rate." @default.
• W2073836386 created "2016-06-24" @default.
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• W2073836386 date "2011-07-16" @default.
• W2073836386 modified "2023-10-04" @default.
• W2073836386 title "Relative expression of genes encoding SMAD signal transduction factors in human granulosa cells is correlated with oocyte quality" @default.
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• W2073836386 doi "https://doi.org/10.1007/s10815-011-9609-6" @default.
• W2073836386 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3220436" @default.
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• W2073836386 hasPublicationYear "2011" @default.
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