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• W2073866295 abstract "Fatty acid elongation defective mutants were isolated from <i>Saccharomyces cerevisiae</i> by mutagenizing strains that were defective in fatty acid synthase (<i>FAS</i>) activity. Cells of the fatty acid synthase-defective strains can grow when supplemented with tetradecanoic acid (14:0) due to the presence of membrane bound elongation systems that can extend the 14 carbon fatty acid to longer chain species. After mutagenesis and rescue on medium containing a mixture of 14:0, 16:0 and 18:0, cells were screened for their inability to grow on medium containing only 14:0. From 150,000 colonies, four stable isolates were identified, all of which appear to represent the same complementation group. Gas chromatography of lipid extracts from mutant <i>elo1-1</i> (designated as elongation defective) cells grown with long or medium chain fatty acids indicates that it fails to efficiently elongate (12, 13, or 14) carbon fatty acids. A gene disrupted <i>fas2Δ::LEU2;elo1Δ::HIS3</i> mutant incorporates 14-18-carbon fatty acids into membrane lipids, indicating that fatty acid transport is not affected by the mutation. Molecular cloning and sequence analysis of the <i>ELO1</i> gene suggests that the encoded protein is a membrane bound polypeptide that contains at least five potential membrane spanning regions and a presumptive NADPH binding site. Analysis of the <i>ELO1</i> mRNA levels indicates that the gene is expressed in cells grown on fatty acid deficient medium. It is rapidly induced in wild type cells that are supplemented with 14:0 and is repressed when cells are supplied with 16- and 18-carbon fatty acids." @default.
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• W2073866295 date "1996-08-01" @default.
• W2073866295 modified "2023-10-14" @default.
• W2073866295 title "Isolation and Characterization of a Gene Affecting Fatty Acid Elongation in Saccharomyces cerevisiae" @default.
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• W2073866295 doi "https://doi.org/10.1074/jbc.271.31.18413" @default.
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