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- W2073868906 abstract "HCV immunological assays have limited specificity due to considerable variability of genomic coding sequences. Accordingly, PCR RNA detection also shows variable incidence of HCV in a non-A, non-B (NANB) hepatitis context. We used in-house designed nested PCR applying primers from the 5′ untranslated region in 150 thalassemic patients classified in four groups according to anti-HCV screening and glutamic-pyruvate transaminase (GPT) levels. Group A: anti-HCV+/high GPT levels; group B: anti-HCV+/normal GPT levels; group C: anti-HCV−/high GPT levels; group D: anti-HCV−/normal GPT levels. Viral incidence and concentration, both high in group A, decreased towards group D. Group C RNA incidence was unexpectedly high and, moreover, one control case proved HCV-RNA+. Compared with the Amplicor kit our primers were considerably more sensitive." @default.
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- W2073868906 date "1997-02-01" @default.
- W2073868906 modified "2023-09-25" @default.
- W2073868906 title "Effective detection of active HCV infection: HCV RNA carrier state in a context free of hepatitis symptoms" @default.
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- W2073868906 doi "https://doi.org/10.1016/s0009-8981(96)06448-0" @default.
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