Matches in SemOpenAlex for { <https://semopenalex.org/work/W2073907360> ?p ?o ?g. }
- W2073907360 endingPage "3397" @default.
- W2073907360 startingPage "3386" @default.
- W2073907360 abstract "MicA is a small non-coding RNA that regulates ompA mRNA translation in Escherichia coli . MicA has an inhibitory function, base pairing to the translation initiation region of target mRNAs through short sequences of complementarity, blocking their ribosome-binding sites. The MicA structure contains two stem loops, which impede its interaction with target mRNAs, and it is thought that the RNA chaperone protein Hfq, known to be involved in MicA regulation of ompA , may structurally remodel MicA to reveal the ompA- binding site for cognate pairing. To further characterize these interactions, we undertook biochemical and biophysical studies using native MicA and a ‘stabilized’ version, modified to mimic the conformational state of MicA where the ompA -binding site is exposed. Our data corroborate two proposed roles for Hfq: first, to bring both MicA and ompA into close proximity, and second, to restructure MicA to allow exposure of the ompA -binding site for pairing, thereby demonstrating the RNA chaperone function of Hfq. Additionally, at accumulated MicA levels, we identified a Mg 2+ -dependent self-association that occludes the ompA -recognition region. We discuss the potential contribution of an Mg 2+ -mediated conformational switch of MicA for the regulation of MicA function." @default.
- W2073907360 created "2016-06-24" @default.
- W2073907360 creator A5003240144 @default.
- W2073907360 creator A5042312368 @default.
- W2073907360 creator A5056451125 @default.
- W2073907360 creator A5067855805 @default.
- W2073907360 creator A5078106860 @default.
- W2073907360 creator A5081424759 @default.
- W2073907360 creator A5082749348 @default.
- W2073907360 date "2013-01-29" @default.
- W2073907360 modified "2023-10-04" @default.
- W2073907360 title "Characterization of MicA interactions suggests a potential novel means of gene regulation by small non-coding RNAs" @default.
- W2073907360 cites W1480821243 @default.
- W2073907360 cites W1489905936 @default.
- W2073907360 cites W1525467358 @default.
- W2073907360 cites W1970713123 @default.
- W2073907360 cites W1974863064 @default.
- W2073907360 cites W1975228919 @default.
- W2073907360 cites W1977038370 @default.
- W2073907360 cites W1982126789 @default.
- W2073907360 cites W1986385237 @default.
- W2073907360 cites W1995211243 @default.
- W2073907360 cites W1997374378 @default.
- W2073907360 cites W2012084705 @default.
- W2073907360 cites W2015943470 @default.
- W2073907360 cites W2029648151 @default.
- W2073907360 cites W2032427999 @default.
- W2073907360 cites W2036107776 @default.
- W2073907360 cites W2037771395 @default.
- W2073907360 cites W2041025550 @default.
- W2073907360 cites W2045533711 @default.
- W2073907360 cites W2050091361 @default.
- W2073907360 cites W2050135538 @default.
- W2073907360 cites W2054260432 @default.
- W2073907360 cites W2065225563 @default.
- W2073907360 cites W2065350472 @default.
- W2073907360 cites W2094029910 @default.
- W2073907360 cites W2095446238 @default.
- W2073907360 cites W2103990238 @default.
- W2073907360 cites W2107713942 @default.
- W2073907360 cites W2111151184 @default.
- W2073907360 cites W2118400513 @default.
- W2073907360 cites W2124983865 @default.
- W2073907360 cites W2130972012 @default.
- W2073907360 cites W2131605202 @default.
- W2073907360 cites W2136139129 @default.
- W2073907360 cites W2136175548 @default.
- W2073907360 cites W2136659582 @default.
- W2073907360 cites W2137897851 @default.
- W2073907360 cites W2139823369 @default.
- W2073907360 cites W2140340428 @default.
- W2073907360 cites W2141157874 @default.
- W2073907360 cites W2145962307 @default.
- W2073907360 cites W2149689268 @default.
- W2073907360 cites W2149699755 @default.
- W2073907360 cites W2150394101 @default.
- W2073907360 cites W2164855461 @default.
- W2073907360 cites W2166639289 @default.
- W2073907360 cites W2170875797 @default.
- W2073907360 cites W236057538 @default.
- W2073907360 doi "https://doi.org/10.1093/nar/gkt008" @default.
- W2073907360 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3597676" @default.
- W2073907360 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23361466" @default.
- W2073907360 hasPublicationYear "2013" @default.
- W2073907360 type Work @default.
- W2073907360 sameAs 2073907360 @default.
- W2073907360 citedByCount "12" @default.
- W2073907360 countsByYear W20739073602013 @default.
- W2073907360 countsByYear W20739073602015 @default.
- W2073907360 countsByYear W20739073602016 @default.
- W2073907360 countsByYear W20739073602017 @default.
- W2073907360 countsByYear W20739073602018 @default.
- W2073907360 countsByYear W20739073602020 @default.
- W2073907360 countsByYear W20739073602021 @default.
- W2073907360 countsByYear W20739073602022 @default.
- W2073907360 countsByYear W20739073602023 @default.
- W2073907360 crossrefType "journal-article" @default.
- W2073907360 hasAuthorship W2073907360A5003240144 @default.
- W2073907360 hasAuthorship W2073907360A5042312368 @default.
- W2073907360 hasAuthorship W2073907360A5056451125 @default.
- W2073907360 hasAuthorship W2073907360A5067855805 @default.
- W2073907360 hasAuthorship W2073907360A5078106860 @default.
- W2073907360 hasAuthorship W2073907360A5081424759 @default.
- W2073907360 hasAuthorship W2073907360A5082749348 @default.
- W2073907360 hasBestOaLocation W20739073601 @default.
- W2073907360 hasConcept C104317684 @default.
- W2073907360 hasConcept C105580179 @default.
- W2073907360 hasConcept C12554922 @default.
- W2073907360 hasConcept C142724271 @default.
- W2073907360 hasConcept C148625055 @default.
- W2073907360 hasConcept C149364088 @default.
- W2073907360 hasConcept C151730666 @default.
- W2073907360 hasConcept C185581394 @default.
- W2073907360 hasConcept C194993378 @default.
- W2073907360 hasConcept C2775962898 @default.
- W2073907360 hasConcept C2781038479 @default.
- W2073907360 hasConcept C4718897 @default.
- W2073907360 hasConcept C54355233 @default.