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- W2073952852 abstract "A new technique for the cryopreservation of cell lines of hemopoietic origin is described. It uses a combination of 10% polyvinylpyrrolidone or hydroxyethylstarch with 15% glycerol, a slow stepwise addition of the cryoprotectants before freezing, cooling at 4 degrees C/min, rapid rewarming, and a slow step-wise dilution after thawing. This technique has given improved recovery rates with a number of basophil/mast cell lines, as well as a wide range of hemopoietic cells including hybridomas." @default.
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- W2073952852 date "1985-10-01" @default.
- W2073952852 modified "2023-10-16" @default.
- W2073952852 title "An improved preservation technique for cells of hemopoietic origin" @default.
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- W2073952852 doi "https://doi.org/10.1016/0011-2240(85)90162-2" @default.
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