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• W2074001943 endingPage "929" @default.
• W2074001943 startingPage "925" @default.
• W2074001943 abstract "An experiment designed to collect a saturation transfer double difference (STDD) NMR spectrum using a solenoid microcoil NMR difference probe is reported. STDD-NMR allows the investigation of ligand-biomolecule binding, with moderate concentration requirements for unlabeled molecular targets and the ability to discern binding events in the presence of non-binding ligands. The NMR difference probe acquires the signals from two different samples at once, and cancels common signals automatically through a mechanism of switching between parallel excitation and serial acquisition of the sample signals. STDD spectra were acquired on a system consisting of human serum albumin and two ligands, octanoic acid and glucose. The non-binding ligand, glucose, was cancelled internally through phase cycling, while the protein signal was subtracted automatically by the difference probe. The proton NMR resonance signal from octanoic acid remained in the double difference spectrum. This work demonstrates that the double difference can be performed both internally and automatically through the utilization of the solenoid microcoil NMR difference probe and STDD-NMR pulse sequence, resulting in a clean signal from the binding ligand with good protein background subtraction and an overall favorable result when compared to the conventional approach." @default.
• W2074001943 created "2016-06-24" @default.
• W2074001943 creator A5002216238 @default.
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• W2074001943 date "2008-07-10" @default.
• W2074001943 modified "2023-10-16" @default.
• W2074001943 title "Saturation transfer double‐difference NMR spectroscopy using a dual solenoid microcoil difference probe" @default.
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• W2074001943 doi "https://doi.org/10.1002/mrc.2275" @default.
• W2074001943 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5441454" @default.
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• W2074001943 hasPublicationYear "2008" @default.
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