Matches in SemOpenAlex for { <https://semopenalex.org/work/W2074020261> ?p ?o ?g. }
- W2074020261 endingPage "4291" @default.
- W2074020261 startingPage "4291" @default.
- W2074020261 abstract "purpose. Proliferative vitreoretinopathy (PVR), a major reason for failure of retinal detachment surgery, is characterized by the formation of scarlike tissue that contains transdifferentiated retinal pigment epithelial (RPE) cells. The scar tissue occurs in response to growth factors such as transforming growth factor (TGF)-β and epidermal growth factor (EGF). The authors postulate that transdifferentiation of RPE cells may arise via epithelial-to-mesenchymal transition (EMT). Bone morphogenetic proteins (BMPs) are expressed in the retina and have an antiproliferative role. Gremlin is expressed in the outer retina and is a BMP antagonist. The study was conducted to establish a model of PVR by inducing EMT in the human RPE cell line ARPE-19, using TGF-β and EGF and to establish the contribution of gremlin to EMT. methods. ARPE-19 cells were cultured and stimulated with TGF-β1, EGF, and gremlin. The expression of α-smooth muscle actin (α-SMA), vimentin, and zona occludens (ZO)-1 were examined via PCR, Western blot analysis, and immunofluorescence. Zymography was performed for matrix metalloproteinase (MMP) activity. Scratch assays were performed to assess migration. results. A model of EMT was established in the ARPE-19 cell line. The characteristics of EMT include gain of α-SMA, loss of ZO-1, upregulation of MMP activity and enhanced migration. Gremlin plays an important role in this process, contributing to the gain of α-SMA, loss of ZO-1, and upregulation of MMP activity. conclusions. EMT occurs in vitro in the ARPE-19 cell line in response to the growth factors TGF-β1 and EGF. EMT is also induced by Gremlin." @default.
- W2074020261 created "2016-06-24" @default.
- W2074020261 creator A5010480920 @default.
- W2074020261 creator A5063656484 @default.
- W2074020261 creator A5065684610 @default.
- W2074020261 creator A5089342465 @default.
- W2074020261 date "2007-09-01" @default.
- W2074020261 modified "2023-09-27" @default.
- W2074020261 title "The Role of Gremlin, a BMP Antagonist, and Epithelial-to-Mesenchymal Transition in Proliferative Vitreoretinopathy" @default.
- W2074020261 cites W1498451757 @default.
- W2074020261 cites W1499500424 @default.
- W2074020261 cites W1531951330 @default.
- W2074020261 cites W1565817365 @default.
- W2074020261 cites W157692930 @default.
- W2074020261 cites W1593721266 @default.
- W2074020261 cites W1966022628 @default.
- W2074020261 cites W1969023545 @default.
- W2074020261 cites W1978202084 @default.
- W2074020261 cites W1980480613 @default.
- W2074020261 cites W1984223367 @default.
- W2074020261 cites W1985301994 @default.
- W2074020261 cites W1985395754 @default.
- W2074020261 cites W1985432260 @default.
- W2074020261 cites W1990724124 @default.
- W2074020261 cites W1992829978 @default.
- W2074020261 cites W1993345123 @default.
- W2074020261 cites W2013259088 @default.
- W2074020261 cites W2015475297 @default.
- W2074020261 cites W2018358706 @default.
- W2074020261 cites W2018817522 @default.
- W2074020261 cites W2028828795 @default.
- W2074020261 cites W2029026343 @default.
- W2074020261 cites W2032942532 @default.
- W2074020261 cites W2033827001 @default.
- W2074020261 cites W2044384686 @default.
- W2074020261 cites W2047393488 @default.
- W2074020261 cites W2051812986 @default.
- W2074020261 cites W2059773069 @default.
- W2074020261 cites W2061476658 @default.
- W2074020261 cites W2063793420 @default.
- W2074020261 cites W2067238776 @default.
- W2074020261 cites W2067400935 @default.
- W2074020261 cites W2073255047 @default.
- W2074020261 cites W2073675430 @default.
- W2074020261 cites W2080786018 @default.
- W2074020261 cites W2088693437 @default.
- W2074020261 cites W2095393157 @default.
- W2074020261 cites W2100837269 @default.
- W2074020261 cites W2106887481 @default.
- W2074020261 cites W2109329533 @default.
- W2074020261 cites W2111731443 @default.
- W2074020261 cites W2112856986 @default.
- W2074020261 cites W2132180445 @default.
- W2074020261 cites W2148184356 @default.
- W2074020261 cites W2150133429 @default.
- W2074020261 cites W2150582334 @default.
- W2074020261 cites W2155579605 @default.
- W2074020261 cites W2161736514 @default.
- W2074020261 cites W2164715245 @default.
- W2074020261 cites W2165874533 @default.
- W2074020261 cites W2167953879 @default.
- W2074020261 cites W2172062181 @default.
- W2074020261 cites W2943812563 @default.
- W2074020261 doi "https://doi.org/10.1167/iovs.07-0086" @default.
- W2074020261 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/17724219" @default.
- W2074020261 hasPublicationYear "2007" @default.
- W2074020261 type Work @default.
- W2074020261 sameAs 2074020261 @default.
- W2074020261 citedByCount "86" @default.
- W2074020261 countsByYear W20740202612012 @default.
- W2074020261 countsByYear W20740202612013 @default.
- W2074020261 countsByYear W20740202612014 @default.
- W2074020261 countsByYear W20740202612015 @default.
- W2074020261 countsByYear W20740202612016 @default.
- W2074020261 countsByYear W20740202612017 @default.
- W2074020261 countsByYear W20740202612018 @default.
- W2074020261 countsByYear W20740202612019 @default.
- W2074020261 countsByYear W20740202612020 @default.
- W2074020261 countsByYear W20740202612021 @default.
- W2074020261 countsByYear W20740202612022 @default.
- W2074020261 countsByYear W20740202612023 @default.
- W2074020261 crossrefType "journal-article" @default.
- W2074020261 hasAuthorship W2074020261A5010480920 @default.
- W2074020261 hasAuthorship W2074020261A5063656484 @default.
- W2074020261 hasAuthorship W2074020261A5065684610 @default.
- W2074020261 hasAuthorship W2074020261A5089342465 @default.
- W2074020261 hasConcept C104317684 @default.
- W2074020261 hasConcept C118131993 @default.
- W2074020261 hasConcept C127561419 @default.
- W2074020261 hasConcept C137738243 @default.
- W2074020261 hasConcept C147447768 @default.
- W2074020261 hasConcept C185592680 @default.
- W2074020261 hasConcept C203014093 @default.
- W2074020261 hasConcept C204232928 @default.
- W2074020261 hasConcept C2776362946 @default.
- W2074020261 hasConcept C2779059073 @default.
- W2074020261 hasConcept C2780428090 @default.
- W2074020261 hasConcept C2780827179 @default.