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- W207405457 abstract "ABSTRACT We describe the cloning and nucleotide sequences of i) three cDNAs encoding postsynaptically-acting neurotoxins and ii) three cDNAs encoding proteins with phospholipase A2 structure [PLA2] present in the venom glands of sea or land snakes. Two of these sequences were already known, but the four others corresponded to hitherto unknown proteins. The signal peptides of the neurotoxins and PLA2 respectively consisted of 21 and 27 amino acid residues. We also describe the production of a hybrid protein molecule comprising the successive sequences of staphylococcal protein A and a postsynaptically-acting neurotoxin. The amount of hybrid produced per liter of culture was in the milligram range. This hybrid protein had biological properties similar to those of native postsynaptically-acting neurotoxins. Antiserum raised in rabbits against this protein afforded protection both In vitro and In vivo, from the harmful effects of the toxin." @default.
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- W207405457 date "1989-01-01" @default.
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- W207405457 title "POSTSYNAPTICALLY-ACTING TOXINS AND PROTEINS WITH PHOSPHOLIPASE STRUCTURE FROM SNAKE VENOMS: COMPLETE AMINO ACID SEQUENCES DEDUCED FROM cDNAs and PRODUCTION OF A TOXIN WITH STAPHYLOCOCCAL PROTEIN A GENE FUSION VECTOR" @default.
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- W207405457 doi "https://doi.org/10.1016/b978-0-08-036139-0.50012-9" @default.
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