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- W2074055128 abstract "Protein patterns were printed using conventional microlithographic materials in a bilayer and a top DNQ/novolak layer. The protein features were printed in both 'contact printing', and 'step and repeat' mode. The latter printing mode can be managed in a single-cell, 'real time' process, as follows: (i) the exposure step is carried out in a cell by focused beam of a confocal microscope tuned to 488nm exposure; (ii) the development step is performed flowing the photoresist developer through the cell; (iii) the selective deposition of the protein is achieved via the flow of the protein solution through the cell until a desired contrast has been reached; (iv) the control step consists of an on-line monitoring of the red fluorescence for the control of the development of the top layer, and of the green fluorescence for the control of the protein patterning, respectively. The techniques have a seamless portability in a biomedical environment, and for 'step and repeat' protein patterning the advantage of a high and controllable resolution. The process can be applied in the fabrication of medical microanalysis devices." @default.
- W2074055128 created "2016-06-24" @default.
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- W2074055128 date "1999-06-11" @default.
- W2074055128 modified "2023-09-23" @default.
- W2074055128 title "Protein patterning using bilayer lithography and confocal microscopy" @default.
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- W2074055128 doi "https://doi.org/10.1117/12.350244" @default.
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