FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2074069116> ?p ?o ?g. }

• W2074069116 endingPage "118" @default.
• W2074069116 startingPage "115" @default.
• W2074069116 abstract "A multiplex polymerase chain reaction (PCR) assay that can simultaneously detect 4 major Vibrio spp., Vibrio alginolyticus, Vibrio parahaemolyticus, Vibrio vulnificus, and Vibrio cholerae, in the presence of an internal amplification control (IAC) was developed. Species-specific PCR primers were designed based on the gyrB gene for V. alginolyticus, the collagenase gene for V. parahaemolyticus, the vvhA gene for V. vulnificus, and the ompW gene for V. cholerae. Additionally, an IAC primer pair was designed in conserved regions of the bacterial 16S rRNA gene that is used to indicate false-negative results. A multiplex PCR method was developed after optimization of the reaction conditions. The specificity of the PCR was validated by using 83 Vibrio strains and 10 other non-Vibrio bacterial species. The detection limit of the PCR was 10 CFU per tube for V. alginolyticus, V. parahaemolyticus, V. vulnificus, and 105 CFU per tube for V. cholerae in mixed conditions. This method was used to identify 69 suspicious Vibrio isolates, and the results were consistent with physiological and biochemical tests. This multiplex PCR method proved to be rapid, sensitive, and specific. The existence of IAC could successfully eliminate false-negative results for the detection of V. alginolyticus, V. parahaemolyticus, V. vulnificus, and V. cholerae." @default.
• W2074069116 created "2016-06-24" @default.
• W2074069116 creator A5008116183 @default.
• W2074069116 creator A5037259290 @default.
• W2074069116 creator A5037473430 @default.
• W2074069116 creator A5075804518 @default.
• W2074069116 creator A5077853155 @default.
• W2074069116 date "2014-06-01" @default.
• W2074069116 modified "2023-10-17" @default.
• W2074069116 title "Multiplex PCR assays for the detection of Vibrio alginolyticus, Vibrio parahaemolyticus, Vibrio vulnificus, and Vibrio cholerae with an internal amplification control" @default.
• W2074069116 cites W1493952493 @default.
• W2074069116 cites W1506123654 @default.
• W2074069116 cites W1530482301 @default.
• W2074069116 cites W1537282737 @default.
• W2074069116 cites W1749128330 @default.
• W2074069116 cites W1966649436 @default.
• W2074069116 cites W1972753109 @default.
• W2074069116 cites W2031944400 @default.
• W2074069116 cites W2033837442 @default.
• W2074069116 cites W2034509070 @default.
• W2074069116 cites W2037220611 @default.
• W2074069116 cites W2066077786 @default.
• W2074069116 cites W2071336932 @default.
• W2074069116 cites W2079489537 @default.
• W2074069116 cites W2081588987 @default.
• W2074069116 cites W2081943558 @default.
• W2074069116 cites W2095989309 @default.
• W2074069116 cites W2096298665 @default.
• W2074069116 cites W2104145784 @default.
• W2074069116 cites W2108026550 @default.
• W2074069116 cites W2141107702 @default.
• W2074069116 cites W2150783010 @default.
• W2074069116 doi "https://doi.org/10.1016/j.diagmicrobio.2014.03.012" @default.
• W2074069116 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/24731836" @default.
• W2074069116 hasPublicationYear "2014" @default.
• W2074069116 type Work @default.
• W2074069116 sameAs 2074069116 @default.
• W2074069116 citedByCount "91" @default.
• W2074069116 countsByYear W20740691162014 @default.
• W2074069116 countsByYear W20740691162016 @default.
• W2074069116 countsByYear W20740691162017 @default.
• W2074069116 countsByYear W20740691162018 @default.
• W2074069116 countsByYear W20740691162019 @default.
• W2074069116 countsByYear W20740691162020 @default.
• W2074069116 countsByYear W20740691162021 @default.
• W2074069116 countsByYear W20740691162022 @default.
• W2074069116 countsByYear W20740691162023 @default.
• W2074069116 crossrefType "journal-article" @default.
• W2074069116 hasAuthorship W2074069116A5008116183 @default.
• W2074069116 hasAuthorship W2074069116A5037259290 @default.
• W2074069116 hasAuthorship W2074069116A5037473430 @default.
• W2074069116 hasAuthorship W2074069116A5075804518 @default.
• W2074069116 hasAuthorship W2074069116A5077853155 @default.
• W2074069116 hasConcept C104317684 @default.
• W2074069116 hasConcept C2776154503 @default.
• W2074069116 hasConcept C2776422119 @default.
• W2074069116 hasConcept C2776575720 @default.
• W2074069116 hasConcept C2778458500 @default.
• W2074069116 hasConcept C2778643871 @default.
• W2074069116 hasConcept C2778968793 @default.
• W2074069116 hasConcept C49105822 @default.
• W2074069116 hasConcept C523546767 @default.
• W2074069116 hasConcept C54355233 @default.
• W2074069116 hasConcept C86803240 @default.
• W2074069116 hasConcept C89423630 @default.
• W2074069116 hasConcept C90583042 @default.
• W2074069116 hasConceptScore W2074069116C104317684 @default.
• W2074069116 hasConceptScore W2074069116C2776154503 @default.
• W2074069116 hasConceptScore W2074069116C2776422119 @default.
• W2074069116 hasConceptScore W2074069116C2776575720 @default.
• W2074069116 hasConceptScore W2074069116C2778458500 @default.
• W2074069116 hasConceptScore W2074069116C2778643871 @default.
• W2074069116 hasConceptScore W2074069116C2778968793 @default.
• W2074069116 hasConceptScore W2074069116C49105822 @default.
• W2074069116 hasConceptScore W2074069116C523546767 @default.
• W2074069116 hasConceptScore W2074069116C54355233 @default.
• W2074069116 hasConceptScore W2074069116C86803240 @default.
• W2074069116 hasConceptScore W2074069116C89423630 @default.
• W2074069116 hasConceptScore W2074069116C90583042 @default.
• W2074069116 hasIssue "2" @default.
• W2074069116 hasLocation W20740691161 @default.
• W2074069116 hasLocation W20740691162 @default.
• W2074069116 hasOpenAccess W2074069116 @default.
• W2074069116 hasPrimaryLocation W20740691161 @default.
• W2074069116 hasRelatedWork W2049804228 @default.
• W2074069116 hasRelatedWork W2114548303 @default.
• W2074069116 hasRelatedWork W2289830784 @default.
• W2074069116 hasRelatedWork W2394792867 @default.
• W2074069116 hasRelatedWork W2549762511 @default.
• W2074069116 hasRelatedWork W2890815809 @default.
• W2074069116 hasRelatedWork W2948140976 @default.
• W2074069116 hasRelatedWork W3047442265 @default.
• W2074069116 hasRelatedWork W3159119623 @default.
• W2074069116 hasRelatedWork W4281862186 @default.
• W2074069116 hasVolume "79" @default.
• W2074069116 isParatext "false" @default.
• W2074069116 isRetracted "false" @default.
• W2074069116 magId "2074069116" @default.

• next