FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2074095909> ?p ?o ?g. }

• W2074095909 endingPage "113" @default.
• W2074095909 startingPage "101" @default.
• W2074095909 abstract "In this study, we tested a hypothesis that activation of calcineurin, Ca2+/calmodulin-dependent protein phosphatase 2B, is an initiating signal for synaptic vesicle endocytosis. We examined effects of calcineurin inhibitors, cyclosporin A or FK506 and calmodulin inhibitors on stimulus-induced FM1-43 uptake into nerve terminals of Drosophila larvae. Fluorescent FM1-43 labels recycling synaptic vesicles in nerve terminals. Pretreatment with cyclosporin A (5-40 microM) or with FK506 (5-10 microM) enhanced FM1-43 uptake induced by high (60 mM) K+ in a dose-dependent manner. The effect required some preincubation time of about 10 min. The nerve terminals loaded with FM1-43 were destained by electrical nerve stimulation in the cyclosporin A-pretreated preparations, confirming that FM1-43 was taken up into synaptic vesicles. Pretreatment with rapamycin (2 or 20 microM), a structural analog of FK506 which has no effect on calcineurin, or calyculin A (0.3-50 nM), an inhibitor of protein phosphatase 1 and 2A, had no detectable effect on FM1-43 uptake. On the other hand, pretreatment with trifluoperazine (1-50 microM) or with phenoxybenzamine (100 microM), inhibitors of calmodulin, enhanced FM1-43 uptake. Since endocytosis is coupled with exocytosis, it is possible that the enhancement of FM1-43 uptake results from facilitation of exocytosis. However, the frequency of spontaneous junctional potentials and the mean amplitude of evoked potentials did not change after the cyclosporin A treatment, suggesting that the exocytosis process was not significantly affected by the drug. Furthermore, we can temporally separate synaptic vesicle exocytosis and endocytosis in a Drosophila mutant, shibire (shi(ts1)). By taking advantage of this mutation, we showed that cyclosporin A and trifluoperazine enhanced synaptic vesicle recycling by directly acting on the endocytotic process. Present results are not compatible with the hypothesis, but suggest that calcineurin inhibits synaptic vesicle recycling." @default.
• W2074095909 created "2016-06-24" @default.
• W2074095909 creator A5013761124 @default.
• W2074095909 creator A5057017831 @default.
• W2074095909 creator A5083466248 @default.
• W2074095909 date "1997-02-01" @default.
• W2074095909 modified "2023-10-17" @default.
• W2074095909 title "An inhibitory role of calcineurin in endocytosis of synaptic vesicles at nerve terminals of Drosophila larvae" @default.
• W2074095909 cites W1509834006 @default.
• W2074095909 cites W1520028124 @default.
• W2074095909 cites W1524619698 @default.
• W2074095909 cites W1574831557 @default.
• W2074095909 cites W1700879768 @default.
• W2074095909 cites W1836738800 @default.
• W2074095909 cites W1940372664 @default.
• W2074095909 cites W1961025744 @default.
• W2074095909 cites W1961999388 @default.
• W2074095909 cites W1967965496 @default.
• W2074095909 cites W1972322271 @default.
• W2074095909 cites W1974337917 @default.
• W2074095909 cites W1978879981 @default.
• W2074095909 cites W1984754431 @default.
• W2074095909 cites W1999090451 @default.
• W2074095909 cites W2002418745 @default.
• W2074095909 cites W2010482889 @default.
• W2074095909 cites W2019386320 @default.
• W2074095909 cites W2023375612 @default.
• W2074095909 cites W2025684146 @default.
• W2074095909 cites W2035149692 @default.
• W2074095909 cites W2037554635 @default.
• W2074095909 cites W2037956598 @default.
• W2074095909 cites W2040987875 @default.
• W2074095909 cites W2055473408 @default.
• W2074095909 cites W2061769295 @default.
• W2074095909 cites W2067529086 @default.
• W2074095909 cites W2068756591 @default.
• W2074095909 cites W2069257334 @default.
• W2074095909 cites W2082106253 @default.
• W2074095909 cites W2084977836 @default.
• W2074095909 cites W2087925323 @default.
• W2074095909 cites W2091347201 @default.
• W2074095909 cites W2093613393 @default.
• W2074095909 cites W2101746871 @default.
• W2074095909 cites W2106104578 @default.
• W2074095909 cites W2108794906 @default.
• W2074095909 cites W2139341460 @default.
• W2074095909 cites W2172095199 @default.
• W2074095909 cites W2174107598 @default.
• W2074095909 cites W2178456244 @default.
• W2074095909 cites W2275222709 @default.
• W2074095909 cites W2343603404 @default.
• W2074095909 cites W2808792907 @default.
• W2074095909 doi "https://doi.org/10.1016/s0168-0102(96)01132-7" @default.
• W2074095909 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9100252" @default.
• W2074095909 hasPublicationYear "1997" @default.
• W2074095909 type Work @default.
• W2074095909 sameAs 2074095909 @default.
• W2074095909 citedByCount "49" @default.
• W2074095909 countsByYear W20740959092012 @default.
• W2074095909 countsByYear W20740959092013 @default.
• W2074095909 countsByYear W20740959092014 @default.
• W2074095909 countsByYear W20740959092015 @default.
• W2074095909 countsByYear W20740959092017 @default.
• W2074095909 countsByYear W20740959092018 @default.
• W2074095909 countsByYear W20740959092019 @default.
• W2074095909 countsByYear W20740959092022 @default.
• W2074095909 countsByYear W20740959092023 @default.
• W2074095909 crossrefType "journal-article" @default.
• W2074095909 hasAuthorship W2074095909A5013761124 @default.
• W2074095909 hasAuthorship W2074095909A5057017831 @default.
• W2074095909 hasAuthorship W2074095909A5083466248 @default.
• W2074095909 hasConcept C11960822 @default.
• W2074095909 hasConcept C12554922 @default.
• W2074095909 hasConcept C126322002 @default.
• W2074095909 hasConcept C128057223 @default.
• W2074095909 hasConcept C130316041 @default.
• W2074095909 hasConcept C148785051 @default.
• W2074095909 hasConcept C170493617 @default.
• W2074095909 hasConcept C175969161 @default.
• W2074095909 hasConcept C178666793 @default.
• W2074095909 hasConcept C181199279 @default.
• W2074095909 hasConcept C185592680 @default.
• W2074095909 hasConcept C191597335 @default.
• W2074095909 hasConcept C200170125 @default.
• W2074095909 hasConcept C28005876 @default.
• W2074095909 hasConcept C2908668639 @default.
• W2074095909 hasConcept C2911091166 @default.
• W2074095909 hasConcept C29688787 @default.
• W2074095909 hasConcept C41625074 @default.
• W2074095909 hasConcept C55493867 @default.
• W2074095909 hasConcept C71924100 @default.
• W2074095909 hasConcept C79747257 @default.
• W2074095909 hasConcept C86803240 @default.
• W2074095909 hasConcept C95444343 @default.
• W2074095909 hasConceptScore W2074095909C11960822 @default.
• W2074095909 hasConceptScore W2074095909C12554922 @default.
• W2074095909 hasConceptScore W2074095909C126322002 @default.
• W2074095909 hasConceptScore W2074095909C128057223 @default.

• next