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- W2074115402 abstract "In a series of 385 sera obtained from volunteer blood donors positive for the first-generation hepatitis C virus assay (Ortho), the viral genome was detected by polymerase chain reaction (PCR) in 89 sera (23%). Most PCR-positive sera were found positive with the c100-3 neutralisation assay (Abbott) and by two second-generation enzyme immunoassays (Abbott, Ortho). However overall specificity of these assays was rather low. By immunoblotting (Innogenetics and Chiron/Ortho) the specificity could be considerably improved and the best correlation with carrier state was obtained when analysing the results for lane-specific reaction: all 89 viral carriers and only 9 other donors had antibodies against structural ‘core’ epitopes. From the present data we can conclude that in screening a volunteer blood donor population the confirmation of antibodies against ‘core’ epitopes by immunoblotting is strongly associated with viral carriage." @default.
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- W2074115402 date "1992-10-01" @default.
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- W2074115402 title "Confirmation of Hepatitis C Virus Positive Blood Donors by Immunoblotting and Polymerase Chain Reaction" @default.
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- W2074115402 doi "https://doi.org/10.1111/j.1423-0410.1992.tb05100.x" @default.
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