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• W2074129189 abstract "The existence of pseudogenes and the observation of intron loss suggest that RNA can serve as an intermediate in recombination. We used a HIS3 reporter gene to show that RNA-mediated recombination occurs in yeast. Hi+ prototroph formation required transcription and expression of the retrotransposon Ty. Two RNA-mediated recombination events were detected: homologous recombination between the cDNA and plasmid hi sequences, resulting in intron loss, and insertion of the cDNA into the chromosome in the absence of HIS3 homology. The chromosomal Hi+ prototrophs showed many hallmarks of naturally occurring pseudogenes. They integrated at novel sites in the chromosome, lacked introns, and possessed poly(A) tracts. Additionally, their 5'ends corresponded with the site of initiation of the GAL1 transcript." @default.
• W2074129189 created "2016-06-24" @default.
• W2074129189 creator A5004678270 @default.
• W2074129189 creator A5076615826 @default.
• W2074129189 creator A5088969123 @default.
• W2074129189 date "1991-10-18" @default.
• W2074129189 modified "2023-10-18" @default.
• W2074129189 title "RNA-mediated recombination in S. cerevisiae" @default.
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• W2074129189 doi "https://doi.org/10.1016/0092-8674(91)90187-4" @default.
• W2074129189 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/1655280" @default.
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