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- W2074137303 abstract "Gas chromatography (GC) is one of the most widely used methods for separating and quantifying lipids. GC almost exclusively uses methyl ester derivatives of fatty acids in conjunction with a flame ionization detector (FID). Other derivatives are discussed in order to enhance structural information for different lipid classes. GC-FID is also useful for analyzing free fatty acids and partial acylglycerols such as monoacylglycerols, diacylglycerols, and sterols after derivatization, which allow for the characterization of different fats and can be extended to the profiling of triacylglycerols, waxes, or sterol esters using heat-stable stationary phases. Complete separation of common fatty acids is typically achieved by using capillary columns with polar stationary phases but depending on the class of lipids to be separated, further separation and fractionation might be necessary or different columns required. Improvements in GC allow for faster analysis that offers many key benefits, such as increased laboratory productivity." @default.
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- W2074137303 date "2005-11-01" @default.
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- W2074137303 title "Application of a direct transmethylation method to the analysis of fatty acid profile in circulating and cultured cells" @default.
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- W2074137303 doi "https://doi.org/10.1016/j.ab.2005.08.001" @default.
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