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- W2074151519 abstract "Virus adsorption-elution studies and physical measurements were performed on four electro-positive membrane filters (Virosorb 1MDS, Posidyne N66, Zeta plus C-30 and Seitz S). The relative hydrophobicity of the membrane filters was determined by measuring the contact angle of carbon tetrachloride with the filters and by measuring the rate of rise of hexadecane and water in the filters. Viruses adsorbed to the least hydrophobic filter (Virosorb 1MDS) could be eluted by using a solution of salt (NaCI or NaEDTA) alone to disrupt electrostatic interactions between the viruses and the filter. Solutions containing both salt and detergent were required to elute virus adsorbed to two of the more hydrophobic filters (Posidyne N66 and Zeta plus C-30), indicating that both electrostatic and hydrophobic interactions were responsible for viral adsorption to these filters. A two-step procedure for recovering bacteriophages from water was developed that employed different filters for each step. Virosorb 1MDS was used to adsorb indigenous phage in water samples. Adsorbed virus could be recovered by treating the filter with a solution of 1.0 M NaCl. Phages in this solution could be further concentrated by a second adsorption-elution step that used Seitz S filters." @default.
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- W2074151519 date "1986-02-01" @default.
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- W2074151519 title "Comparison of positively charged membrane filters and their use in concentrating bacteriophages in water" @default.
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- W2074151519 doi "https://doi.org/10.1016/0043-1354(86)90004-7" @default.
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