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- W2074195301 abstract "Abstract When assessed electrophysiologically, the sensitivity of Purkinje fibers to the toxic effects of ouabain is greater than that of ventricular muscle fibers. We have attempted to determine in vitro whether this is a reflection of a differential sensitivity of the Na + , K + -ATPase activity. Purkinje fibers and adjacent papillary muscle were homogenized in a medium which prevented the formation of impermeable membrane vesicles, thus preventing ion accumulation and allowing both sides of the membrane to be exposed to ouabaain, substrate, and cofactors. Under these conditions the Na + , K + -ATPase activity from Purkinje fibers was not more sensitive to ouabain compared to the activity from adjacent papillary muscle. The results suggest that factors other than inherent ouabain sensitivity of the Na + , K + -ATPase must underlie the apparent differential ouabain-Na + , K + -ATPase interaction in vivo ." @default.
- W2074195301 created "2016-06-24" @default.
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- W2074195301 date "1978-12-01" @default.
- W2074195301 modified "2023-09-26" @default.
- W2074195301 title "Ouabain sensitivity of the Na+, K+-ATPase activity from single bovine cardiac Purkinje fiber and adjacent papillary muscle" @default.
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- W2074195301 doi "https://doi.org/10.1016/0022-2828(78)90359-0" @default.
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