FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2074213812> ?p ?o ?g. }

• W2074213812 endingPage "1601" @default.
• W2074213812 startingPage "1594" @default.
• W2074213812 abstract "This study describes an approach for genotyping Giardia cysts obtained from wastewater treatment plants (WTPs) in Spain using real-time PCR (qPCR) in combination with immunomagnetic beads.A 50-cycle amplification of a 74-bp fragment of the Giardia beta-giardin gene was adopted from a previous qPCR method. Additionally, two locked nucleic acid (LNA) probes were designed (LNA P434 P1 for assemblage A and LNA P434 H3 for assemblage B). All 16 wastewater samples analysed were positive with the immunofluorescence assay (IFA). Assemblage A was detected in all WTP samples using primer-LNA probe P434 P1 set. Giardia duodenalis identification was confirmed by PCR-RFLP analysis and sequencing of the beta-giardin gene in the water samples found positive by IFA and qPCR. Among the 16 assemblage A isolates that were sequenced, two subtypes were identified; 11 corresponded to the A2 subgenotype, whereas three corresponded to the subgenotype A3. A mixture of subgenotypes was found in the remaining two isolates.The newly developed qPCR assays were able to discern G. duodenalis assemblages A and B in wastewater.The real-time PCR assays provided a rapid method for detection and one-step genotyping of G. duodenalis from wastewater samples, and its application would contribute to understanding the distribution and abundance of G. duodenalis assemblages A and B in wastewater." @default.
• W2074213812 created "2016-06-24" @default.
• W2074213812 creator A5001544428 @default.
• W2074213812 creator A5053251179 @default.
• W2074213812 creator A5053879123 @default.
• W2074213812 date "2010-05-01" @default.
• W2074213812 modified "2023-09-23" @default.
• W2074213812 title "LNA probes in a real-time TaqMan PCR assay for genotyping of<i>Giardia duodenalis</i>in wastewaters" @default.
• W2074213812 cites W1988227922 @default.
• W2074213812 cites W2008183439 @default.
• W2074213812 cites W2029150216 @default.
• W2074213812 cites W2035580712 @default.
• W2074213812 cites W2035931483 @default.
• W2074213812 cites W2039784294 @default.
• W2074213812 cites W2046443850 @default.
• W2074213812 cites W2049308903 @default.
• W2074213812 cites W2053746705 @default.
• W2074213812 cites W2058977096 @default.
• W2074213812 cites W2061696462 @default.
• W2074213812 cites W2071615747 @default.
• W2074213812 cites W2081249972 @default.
• W2074213812 cites W2098160269 @default.
• W2074213812 cites W2100742311 @default.
• W2074213812 cites W2113475228 @default.
• W2074213812 cites W2123269588 @default.
• W2074213812 cites W2127908451 @default.
• W2074213812 cites W2148573794 @default.
• W2074213812 cites W2149607409 @default.
• W2074213812 cites W2150014312 @default.
• W2074213812 cites W2157683553 @default.
• W2074213812 cites W2168235167 @default.
• W2074213812 cites W2170910415 @default.
• W2074213812 cites W2171791043 @default.
• W2074213812 doi "https://doi.org/10.1111/j.1365-2672.2009.04559.x" @default.
• W2074213812 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19840182" @default.
• W2074213812 hasPublicationYear "2010" @default.
• W2074213812 type Work @default.
• W2074213812 sameAs 2074213812 @default.
• W2074213812 citedByCount "12" @default.
• W2074213812 countsByYear W20742138122012 @default.
• W2074213812 countsByYear W20742138122014 @default.
• W2074213812 countsByYear W20742138122015 @default.
• W2074213812 countsByYear W20742138122016 @default.
• W2074213812 countsByYear W20742138122018 @default.
• W2074213812 countsByYear W20742138122022 @default.
• W2074213812 crossrefType "journal-article" @default.
• W2074213812 hasAuthorship W2074213812A5001544428 @default.
• W2074213812 hasAuthorship W2074213812A5053251179 @default.
• W2074213812 hasAuthorship W2074213812A5053879123 @default.
• W2074213812 hasConcept C104317684 @default.
• W2074213812 hasConcept C127413603 @default.
• W2074213812 hasConcept C129312508 @default.
• W2074213812 hasConcept C135763542 @default.
• W2074213812 hasConcept C14363569 @default.
• W2074213812 hasConcept C143660080 @default.
• W2074213812 hasConcept C153911025 @default.
• W2074213812 hasConcept C178790620 @default.
• W2074213812 hasConcept C185592680 @default.
• W2074213812 hasConcept C2777271458 @default.
• W2074213812 hasConcept C2777563447 @default.
• W2074213812 hasConcept C31467283 @default.
• W2074213812 hasConcept C48023723 @default.
• W2074213812 hasConcept C49105822 @default.
• W2074213812 hasConcept C54355233 @default.
• W2074213812 hasConcept C548081761 @default.
• W2074213812 hasConcept C86803240 @default.
• W2074213812 hasConcept C89423630 @default.
• W2074213812 hasConcept C94061648 @default.
• W2074213812 hasConceptScore W2074213812C104317684 @default.
• W2074213812 hasConceptScore W2074213812C127413603 @default.
• W2074213812 hasConceptScore W2074213812C129312508 @default.
• W2074213812 hasConceptScore W2074213812C135763542 @default.
• W2074213812 hasConceptScore W2074213812C14363569 @default.
• W2074213812 hasConceptScore W2074213812C143660080 @default.
• W2074213812 hasConceptScore W2074213812C153911025 @default.
• W2074213812 hasConceptScore W2074213812C178790620 @default.
• W2074213812 hasConceptScore W2074213812C185592680 @default.
• W2074213812 hasConceptScore W2074213812C2777271458 @default.
• W2074213812 hasConceptScore W2074213812C2777563447 @default.
• W2074213812 hasConceptScore W2074213812C31467283 @default.
• W2074213812 hasConceptScore W2074213812C48023723 @default.
• W2074213812 hasConceptScore W2074213812C49105822 @default.
• W2074213812 hasConceptScore W2074213812C54355233 @default.
• W2074213812 hasConceptScore W2074213812C548081761 @default.
• W2074213812 hasConceptScore W2074213812C86803240 @default.
• W2074213812 hasConceptScore W2074213812C89423630 @default.
• W2074213812 hasConceptScore W2074213812C94061648 @default.
• W2074213812 hasIssue "5" @default.
• W2074213812 hasLocation W20742138121 @default.
• W2074213812 hasLocation W20742138122 @default.
• W2074213812 hasOpenAccess W2074213812 @default.
• W2074213812 hasPrimaryLocation W20742138121 @default.
• W2074213812 hasRelatedWork W17396297 @default.
• W2074213812 hasRelatedWork W1934317673 @default.
• W2074213812 hasRelatedWork W2074213812 @default.
• W2074213812 hasRelatedWork W2083277645 @default.
• W2074213812 hasRelatedWork W2119210416 @default.
• W2074213812 hasRelatedWork W2356295664 @default.

• next