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• W2074266952 abstract "The effect of 5-hydroxytryptamine (5HT) on cytoplasmic Ca2+ concentration was examined at the single cell level in astroglial enriched primary cultures from newborn rat cerebral cortex. Type 1 astroglial cells were identified and the Ca2+ indicator dye fura 2/AM was used in a microspectrofluorimetric system. Pharmacological studies indicated that the Ca2+ responses were mediated by 5HT2 receptors. Four different patterns of 5HT evoked cytosolic Ca2+ responses were identified including two different types of spike patterns and two types of Ca2+ oscillations (low amplitude and base-line spiking behaviour). In addition, cells with spontaneous Ca2+ oscillations were of two types, those responding to 5HT and those not responding to 5HT. The different responses were identified already on day 7 in culture and were followed up to day 21 with a concomitant increase in the number of responding cells, although the response patterns did not differ during culture. The triggering 5HT concentration was 1μM. The results suggest that subpopulations of astrocytes exist with respect to 5HT2-evoked cytosolic Ca2+ mobilization." @default.
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• W2074266952 date "1991-01-01" @default.
• W2074266952 modified "2023-10-18" @default.
• W2074266952 title "Heterogeneity among astroglial cells with respect to 5HT-evoked cytosolic Ca2+ responses. A microspectrofluorimetric study on single cells in primary culture" @default.
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• W2074266952 doi "https://doi.org/10.1016/0024-3205(91)90198-k" @default.
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