FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2074279898> ?p ?o ?g. }

• W2074279898 endingPage "32" @default.
• W2074279898 startingPage "23" @default.
• W2074279898 abstract "Horse liver alcohol dehydrogenase was crystallized at pH 7 and 5 °C from an equilibrium mixture containing predominantly NAD+ and p-bromobenzyl alcohol and lower concentrations of NADH and p-bromobenzaldehyde. It is assumed from the results of kinetic studies in solution that most of the enzyme was complexed with NAD+ and p-bromobenzyl alcohol. A ternary complex with NAD+ and trifluoroethanol (an unreactive alcohol) and complexes with NAD + or NADH and 2-methyl-2,4-pentanediol (the crystallization agent, which partially occupies the substrate-binding pocket) were also crystallized. All of these complexes formed triclinic crystals that were isomorphous with the enzyme-NADH-dimethylsulfoxide complex, which has been solved to a resolution of 4.5 Å. X-ray diffractometer data (at 4.5 Å) were used to compute electron density difference maps, which showed that both subunits of the dimeric enzyme bound p-bromobenzyl alcohol or trifluoroethanol in a substrate binding pocket that was close to the position occupied by dimethylsulfoxide. These complexes appeared to have the same protein and coenzyme structure as the complex with NADH and dimethylsulfoxide. Since the dimethylsulfoxide appeared to be ligated to the catalytic zinc atom, it was not possible to see a direct connection between the alcohols and the zinc ion in these difference maps. However, maps computed from the differences in amplitudes between the NAD+-alcohol complexes and NAD(H)-methylpentanediol complexes and the phases from the NADH-dimethylsulfoxide complex showed continuous electron density from the bromo or trifluoromethyl positions of the substrates to the zinc ion. Using the structure of the orthorhombic form of apoenzyme, which has been solved to a resolution of 2.4 Å, as a guide for interpreting the various maps, we constructed a provisional model of the active site. This model led us to conclude that the oxygen of either alcohol is directly ligated to the zinc ion (2 Å), while carbon 1 of the alcohol is 3.5 Å from carbon 4 of the nicotinamide ring. Studies at higher resolution are required to confirm these conclusions and to reveal further details." @default.
• W2074279898 created "2016-06-24" @default.
• W2074279898 creator A5015206461 @default.
• W2074279898 creator A5050446822 @default.
• W2074279898 creator A5079928889 @default.
• W2074279898 date "1978-06-01" @default.
• W2074279898 modified "2023-09-27" @default.
• W2074279898 title "Crystallography of liver alcohol dehydrogenase complexed with substrates" @default.
• W2074279898 cites W132282532 @default.
• W2074279898 cites W1496711114 @default.
• W2074279898 cites W1510248076 @default.
• W2074279898 cites W1518175319 @default.
• W2074279898 cites W1533946522 @default.
• W2074279898 cites W1561690684 @default.
• W2074279898 cites W15864163 @default.
• W2074279898 cites W1597564353 @default.
• W2074279898 cites W173174986 @default.
• W2074279898 cites W1982488376 @default.
• W2074279898 cites W1987549653 @default.
• W2074279898 cites W1987941631 @default.
• W2074279898 cites W2003424499 @default.
• W2074279898 cites W2017247695 @default.
• W2074279898 cites W2017774801 @default.
• W2074279898 cites W2026854579 @default.
• W2074279898 cites W2027017124 @default.
• W2074279898 cites W2027074254 @default.
• W2074279898 cites W2035626564 @default.
• W2074279898 cites W2039557588 @default.
• W2074279898 cites W2047583459 @default.
• W2074279898 cites W2057317445 @default.
• W2074279898 cites W2060183170 @default.
• W2074279898 cites W2066592844 @default.
• W2074279898 cites W2069439613 @default.
• W2074279898 cites W2077742005 @default.
• W2074279898 cites W2091021757 @default.
• W2074279898 cites W2093514476 @default.
• W2074279898 cites W4246090862 @default.
• W2074279898 cites W2304195674 @default.
• W2074279898 doi "https://doi.org/10.1016/0022-2836(78)90105-5" @default.
• W2074279898 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/209195" @default.
• W2074279898 hasPublicationYear "1978" @default.
• W2074279898 type Work @default.
• W2074279898 sameAs 2074279898 @default.
• W2074279898 citedByCount "79" @default.
• W2074279898 countsByYear W20742798982012 @default.
• W2074279898 countsByYear W20742798982013 @default.
• W2074279898 countsByYear W20742798982016 @default.
• W2074279898 countsByYear W20742798982017 @default.
• W2074279898 countsByYear W20742798982021 @default.
• W2074279898 countsByYear W20742798982023 @default.
• W2074279898 crossrefType "journal-article" @default.
• W2074279898 hasAuthorship W2074279898A5015206461 @default.
• W2074279898 hasAuthorship W2074279898A5050446822 @default.
• W2074279898 hasAuthorship W2074279898A5079928889 @default.
• W2074279898 hasConcept C115624301 @default.
• W2074279898 hasConcept C178790620 @default.
• W2074279898 hasConcept C181199279 @default.
• W2074279898 hasConcept C185592680 @default.
• W2074279898 hasConcept C18903297 @default.
• W2074279898 hasConcept C197957613 @default.
• W2074279898 hasConcept C2777289219 @default.
• W2074279898 hasConcept C2777344606 @default.
• W2074279898 hasConcept C2781066024 @default.
• W2074279898 hasConcept C42228675 @default.
• W2074279898 hasConcept C535196362 @default.
• W2074279898 hasConcept C71240020 @default.
• W2074279898 hasConcept C75520062 @default.
• W2074279898 hasConcept C8010536 @default.
• W2074279898 hasConcept C86756495 @default.
• W2074279898 hasConcept C86803240 @default.
• W2074279898 hasConceptScore W2074279898C115624301 @default.
• W2074279898 hasConceptScore W2074279898C178790620 @default.
• W2074279898 hasConceptScore W2074279898C181199279 @default.
• W2074279898 hasConceptScore W2074279898C185592680 @default.
• W2074279898 hasConceptScore W2074279898C18903297 @default.
• W2074279898 hasConceptScore W2074279898C197957613 @default.
• W2074279898 hasConceptScore W2074279898C2777289219 @default.
• W2074279898 hasConceptScore W2074279898C2777344606 @default.
• W2074279898 hasConceptScore W2074279898C2781066024 @default.
• W2074279898 hasConceptScore W2074279898C42228675 @default.
• W2074279898 hasConceptScore W2074279898C535196362 @default.
• W2074279898 hasConceptScore W2074279898C71240020 @default.
• W2074279898 hasConceptScore W2074279898C75520062 @default.
• W2074279898 hasConceptScore W2074279898C8010536 @default.
• W2074279898 hasConceptScore W2074279898C86756495 @default.
• W2074279898 hasConceptScore W2074279898C86803240 @default.
• W2074279898 hasIssue "1" @default.
• W2074279898 hasLocation W20742798981 @default.
• W2074279898 hasLocation W20742798982 @default.
• W2074279898 hasOpenAccess W2074279898 @default.
• W2074279898 hasPrimaryLocation W20742798981 @default.
• W2074279898 hasRelatedWork W1550327100 @default.
• W2074279898 hasRelatedWork W2025659901 @default.
• W2074279898 hasRelatedWork W2035523887 @default.
• W2074279898 hasRelatedWork W2063602979 @default.
• W2074279898 hasRelatedWork W2082729684 @default.
• W2074279898 hasRelatedWork W2087890007 @default.
• W2074279898 hasRelatedWork W2090682483 @default.

• next