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• W2074305938 abstract "During development, the endoderm gives rise to several organs, including the pancreas and liver. This differentiation process requires spatial and temporal regulation of gene expression in the endoderm by a network of tissue-specific transcription factors whose elucidation is far from complete. These factors include the Onecut protein hepatocyte nuclear factor-6 (HNF-6), which controls pancreas and liver development as shown in our previous work on Hnf6 knock-out embryos. In mammals, HNF-6 has two paralogs, Onecut-2 (OC-2) and OC-3, whose patterns of expression in the adult overlap with that of HNF-6. In the present work, we examine the expression profile of the three Onecut factors in the developing mouse endoderm. We show that HNF-6, OC-2, and OC-3 are expressed sequentially, which defines new steps in endoderm differentiation. By analyzing Hnf6 knock-out embryos we find that HNF-6 is required for expression of the Oc3 gene in the endoderm. We show that OC-3 colocalizes with HNF-6 in the endoderm and in embryonic pancreas and liver. Based on transfection, chromatin immunoprecipitation, and whole embryo electroporation experiments, we demonstrate that HNF-6 can bind to and stimulate the expression of the Oc3 gene. This study identifies a regulatory cascade between two paralogous transcription factors, sheds new light on the interpretation of the Hnf6 knock-out phenotype, and broadens the transcription factors network operating during development of the endoderm, liver, and pancreas. During development, the endoderm gives rise to several organs, including the pancreas and liver. This differentiation process requires spatial and temporal regulation of gene expression in the endoderm by a network of tissue-specific transcription factors whose elucidation is far from complete. These factors include the Onecut protein hepatocyte nuclear factor-6 (HNF-6), which controls pancreas and liver development as shown in our previous work on Hnf6 knock-out embryos. In mammals, HNF-6 has two paralogs, Onecut-2 (OC-2) and OC-3, whose patterns of expression in the adult overlap with that of HNF-6. In the present work, we examine the expression profile of the three Onecut factors in the developing mouse endoderm. We show that HNF-6, OC-2, and OC-3 are expressed sequentially, which defines new steps in endoderm differentiation. By analyzing Hnf6 knock-out embryos we find that HNF-6 is required for expression of the Oc3 gene in the endoderm. We show that OC-3 colocalizes with HNF-6 in the endoderm and in embryonic pancreas and liver. Based on transfection, chromatin immunoprecipitation, and whole embryo electroporation experiments, we demonstrate that HNF-6 can bind to and stimulate the expression of the Oc3 gene. This study identifies a regulatory cascade between two paralogous transcription factors, sheds new light on the interpretation of the Hnf6 knock-out phenotype, and broadens the transcription factors network operating during development of the endoderm, liver, and pancreas. In the mouse, cells in the definitive endoderm differentiate from embryonic day 8 (4–6-somite stage) to form the epithelial lining of the primitive gut and associated organs (reviewed in Refs. 1Wells J.M. Melton D.A. Annu. Rev. Cell Dev. Biol. 1999; 15: 393-410Crossref PubMed Scopus (428) Google Scholar and 2Grapin-Botton A. Melton D.A. Trends Genet. 2000; 16: 124-130Abstract Full Text Full Text PDF PubMed Scopus (232) Google Scholar). These organs arise from the gut by budding into the surrounding mesenchyme. This process requires precise signaling, in time and space, from the mesoderm and the regionalized expression of tissue-specific transcription factors in the endoderm. This unique combination of transcription factors in the endoderm delineates a specific transcriptional network that specifies the presumptive organ territories. For instance, during development of the liver and pancreas, two organs that derive from adjacent endodermal regions, a core endodermal program is constituted by the expression of transcription factors such as the FoxA factors, the GATA factors, Hb9, Pdx-1, Hex, HNF-1, 1The abbreviations used are: HNF, hepatocyte nuclear factor; OC, Onecut; e, embryonic day; GFP, green fluorescent protein; BMEL, bipotential mouse embryonic liver; CMV, cytomegalovirus. HNF-4, Nr5a2, and HNF-6 (reviewed in Refs. 3Jensen J. Dev. Dyn. 2004; 229: 176-200Crossref PubMed Scopus (338) Google Scholar and 4Servitja J.M. Ferrer J. Diabetologia. 2004; 47: 597-613Crossref PubMed Scopus (181) Google Scholar). Not all the components of this endodermal program have been identified and the auto- and cross-regulatory loops that control their function are not well understood. Elucidation of this network is required for full understanding of pancreas and liver development and for implementing such knowledge in the controlled differentiation of cells for cell therapy. Hepatocyte nuclear factor-6 (HNF-6) belongs to the Onecut (OC) family of transcription factors (5Lemaigre F.P. Durviaux S.M. Truong O. Lannoy V.J. Hsuan J.J. Rousseau G.G. Proc. Natl. Acad. Sci. U. S. A. 1996; 93: 9460-9464Crossref PubMed Scopus (128) Google Scholar, 6Lannoy V.J. Burglin T.R. Rousseau G.G. Lemaigre F.P. J. Biol. Chem. 1998; 273: 13552-13562Abstract Full Text Full Text PDF PubMed Scopus (92) Google Scholar). These factors possess a single cut domain, hence their name, and a divergent homeodomain, which are both involved in DNA binding. Onecut proteins have been identified not only in humans and rodents, but also in model organisms such as Caenorhabditis elegans (6Lannoy V.J. Burglin T.R. Rousseau G.G. Lemaigre F.P. J. Biol. Chem. 1998; 273: 13552-13562Abstract Full Text Full Text PDF PubMed Scopus (92) Google Scholar), Drosophila melanogaster (7Nguyen D.N. Rohrbaugh M. Lai Z. Mech. Dev. 2000; 97: 57-72Crossref PubMed Scopus (36) Google Scholar), and Danio rerio (8Hong S.-K. Kim C.-H. Yoo K.-W. Kim H.-S. Kudoh T. Dawid I.B. Huh T.-L. Mech. Dev. 2002; 112: 199-202Crossref PubMed Scopus (26) Google Scholar). In addition to Hnf6, mammalian genomes contain two other Onecut genes called Oc2 and Oc3 (9Jacquemin P. Lannoy V.J. Rousseau G.G. Lemaigre F.P. J. Biol. Chem. 1999; 274: 2665-2671Abstract Full Text Full Text PDF PubMed Scopus (86) Google Scholar, 10Vanhorenbeeck V. Jacquemin P. Lemaigre F.P. Rousseau G.G. Biochem. Biophys. Res. Commun. 2002; 292: 848-854Crossref PubMed Scopus (34) Google Scholar). The sequence conservation in their cut and homeodomains suggests that these paralogs may bind the same genes, and indeed many HNF-6 binding sites on DNA are recognized by OC-2 and OC-3 (9Jacquemin P. Lannoy V.J. Rousseau G.G. Lemaigre F.P. J. Biol. Chem. 1999; 274: 2665-2671Abstract Full Text Full Text PDF PubMed Scopus (86) Google Scholar, 10Vanhorenbeeck V. Jacquemin P. Lemaigre F.P. Rousseau G.G. Biochem. Biophys. Res. Commun. 2002; 292: 848-854Crossref PubMed Scopus (34) Google Scholar). During mouse development, HNF-6 is first detected at e8.5 in the foregut-midgut region. As development proceeds, HNF-6 becomes restricted to the liver and the pancreas (11Landry C. Clotman F. Hioki T. Oda H. Picard J.J Lemaigre F.P. Rousseau G.G. Dev. Biol. 1997; 192: 247-257Crossref PubMed Scopus (139) Google Scholar, 12Rausa F. Samadani U. Ye H. Lim L. Fletcher C.F. Jenkins N.A. Copeland N.G. Costa R.H. Dev. Biol. 1997; 192: 228-246Crossref PubMed Scopus (157) Google Scholar). Expression of Oc2 in the endoderm is superimposable to that of Hnf6 (13Jacquemin P. Pierreux C.E. Fierens S. van Eyll J.M. Lemaigre F.P. Rousseau G.G. Gene Expr. Patterns. 2003; 3: 639-644Crossref PubMed Scopus (40) Google Scholar), and later on it is restricted to the liver, pancreas, gut, and stomach. Nothing is known on the endodermal expression of OC-3, but at later stages Oc3 is found in the gut and stomach (10Vanhorenbeeck V. Jacquemin P. Lemaigre F.P. Rousseau G.G. Biochem. Biophys. Res. Commun. 2002; 292: 848-854Crossref PubMed Scopus (34) Google Scholar). Thus, the three Onecut factors share partially overlapping expression domains and may be functionally redundant. Inactivation of the mouse Hnf6 gene revealed that it is important for development of the pancreas at the precursor stage in the endoderm (14Jacquemin P. Lemaigre F.P. Rousseau G.G. Dev. Biol. 2003; 258: 105-116Crossref PubMed Scopus (157) Google Scholar). Thus, Hnf6 knock-out embryos display a pancreatic hypoplasia that results from defects in early pancreas specification. Indeed, the onset of Pdx-1 expression is delayed in the endoderm of Hnf6 knock-out embryos, leading to a reduced number of specified cells in the pancreatic territory at the time of budding. Consistent with this, HNF-6 can bind to and stimulate transcription of the Pdx1 gene (14Jacquemin P. Lemaigre F.P. Rousseau G.G. Dev. Biol. 2003; 258: 105-116Crossref PubMed Scopus (157) Google Scholar). In the present work, we wished to further characterize the transcription factor network in the endoderm. We have examined the time course of expression of the three Onecut factors in the developing endoderm of normal mouse embryos and have investigated whether inactivation of Hnf6 influences the expression of Oc2 and Oc3. We show here that, in the endoderm of Hnf6 knock-out embryos, Oc2 expression is unaffected, but Oc3 expression is abolished. We demonstrate that HNF-6 can induce Oc3 expression and we provide evidence that this is a direct effect. Our work defines new stages in endoderm differentiation and identifies a paralogous regulatory cascade in the Onecut transcription factors, which operates during differentiation of the endoderm, liver, and pancreas. Animals—The Hnf6 knock-out mice, raised in our animal facility and treated according to the principles of laboratory animal care of the University Animal Welfare Committee, were obtained as described (15Jacquemin P. Durviaux S.M. Jensen J. Godfraind C. Gradwohl G. Guillemot F. Madsen O.D. Carmeliet P. Dewerchin M. Collen D. Rousseau G.G. Lemaigre F.P. Mol. Cell. Biol. 2000; 20: 4445-4454Crossref PubMed Scopus (277) Google Scholar). The mice were genotyped by polymerase chain reaction. Dissection, RNA Purification, and Reverse Transcription-coupled PCR—RNA was extracted from tissues with the Tripure RNA Isolation reagent (Roche Diagnostics). Individual embryos at the 5–14-somite stage were dissected in phosphate-buffered saline containing 1.5 mg/ml dispase (Invitrogen) to separate the endoderm layer from the mesoderm and ectoderm. Most of the endoderm, including the hindgut, midgut, and foregut regions, was used for RNA extraction. At e9.5 and e10.5, the region that gives rise to the liver, pancreas, part of the stomach, and gut was dissected from a single embryo. At e12.5, the pancreas or the liver were isolated. Total RNA from transfected cells or from embryos electroporated with plasmid DNA was treated with TURBO DNase I according to the manufacturer (Ambion), to eliminate DNA. Total RNA (up to 1 μg) was reverse transcribed as described (16Clotman F. Lannoy V.J. Reber M. Cereghini S. Cassiman D. Jacquemin P. Roskams T. Rousseau G.G. Lemaigre F.P. Development. 2002; 129: 1819-1828Crossref PubMed Google Scholar). For semiquantitative PCR, the number of cycles for each pair of primers was such that amplification was in the mid-logarithmic phase. Quantitative real time PCR was performed with the SYBR Green PCR Core kit or master mixture (Eurogentec) on a MyIQ thermal cycler (Bio-Rad). Treshold cycles were transformed to copy number according to the standard calibration curve. Absolute copy number for each mRNA was normalized to absolute β-actin mRNA copy number. Primer sequences were 5′-TCCTGAGCGCAAGTACTCTGT-3′ and 5′-CTGATCCACATCTGCTGGAAG-3′ for β-actin, 5′-AGCCCTGGAGCAAACTCAAGT-3′ and 5′-TGCATGTAGAGTTCGACGTTG-3′ for exogenous HNF-6, 5′-TTCCAGCGCATGTCGGCGCTC-3′ and 5′-GGTACTAGTCCGTGGTTCTTC-3′ for endogenous HNF-6, 5′-GCATGTCTGCCTTACGCCTG-3′ and 5′-GCGGGATTTCTTCTGCGAG-3′ for OC-2, 5′-CGGAGTTCCAGCGCATGTC-3′ and 5′-GAGAAGGTGGCAGTAGCTGTC-3′ for OC-3, 5′-AAGAGCCCAACCGCGTCCAGC-3′ and 5′-AGTACGGGTCCTCTTGTTTTC-3′ for Pdx-1, 5′-CCTCGTGACCACCCTGAC-3′ and 5′-CTCAGGTAGTGGTTGTCGG-3′ for green fluorescent protein (GFP), 5′-TGAGGTCGACTGAGAGGTTC-3′ and 5′-TGTGCCCCCTGAGCCCCAAG-3′ for Oc3 region a, 5′-AAGTCTGGACAGTCTGCAAG-3′ and 5′-TACCAGCCACCCCGGGATTC-3′ for Oc3 region b, 5′-CAACCCTCTGCCCAGGAATC-3′ and 5′-GCCAGGATCAGAGTCAAGTC-3′ for Oc3 region c. Antibodies and Immunofluorescence—Anti-OC-3 antibodies were raised against the N-terminal moiety of mouse OC-3. Amino acids 23 to 333 of OC-3 were fused downstream of the glutathione S-transferase coding sequence in the pGEX-CS vector (17Parks T.D. Leuther K.K. Howard E.D. Johnston S.A. Dougherty W.G. Anal. Biochem. 1994; 216: 413-417Crossref PubMed Scopus (258) Google Scholar) and produced in Escherichia coli. The fusion protein was purified using glutathione and extracted from colloidal blue-stained SDS-PAGE before injection to guinea pigs. Their serum was used as a source of anti-OC-3 antibodies. For immunofluorescence, embryos were fixed at 4 °C in 4% paraformaldehyde in phosphate-buffered saline for 1 h. Embryos were embedded and immunofluorescence was detected as described (18van Eyll J.M. Pierreux C.E. Lemaigre F.P. Rousseau G.G. J. Cell Sci. 2004; 117: 2077-2086Crossref PubMed Scopus (43) Google Scholar). Primary antibodies and dilutions were as follows: monoclonal mouse anti-E-cadherin at 1:50 (BD Transduction Laboratories), rabbit anti-HNF-6 at 1:25 (Santa Cruz), rabbit anti-Pdx-1 at 1:1000 (a kind gift from C. V. Wright), rabbit anti-Prox1 at 1:1000 (Covance), and guinea pig anti-OC-3 at 1:4000. Primary antibodies were detected by immunofluorescence using biotinylated sheep anti-rabbit immunoglobulin G (Roche Diagnostics) followed by streptavidin-Alexa Fluor 488 conjugate (Molecular Probes), using a goat anti-mouse antibody coupled to Texas Red (Jackson) or using a goat anti-guinea pig antibody coupled to Alexa Fluor 594 (Molecular Probes). Sections were analyzed with a Zeiss Axiovert 200 fluorescence microscope. For immunohistochemistry, primary antibodies were detected by immunoperoxidase labeling using biotinylated sheep anti-rabbit or anti-guinea pig immunoglobulin G (Jackson ImmunoResearch) and DAB+ (Dako). Pictures were taken using a Coolpix 995 digital camera (Nikon). Cell Culture and Transfections—Bipotential embryonic mouse liver (BMEL) cells were maintained in Dulbecco's modified Eagle's medium supplemented with 10% fetal calf serum, insulin-like growth factor II, insulin, and EGF according to Strick-Marchand and colleagues (19Strick-Marchand H. Weiss M.C. Hepatology. 2002; 36: 794-804Crossref PubMed Google Scholar, 20Strick-Marchand H. Moresan S. Charneau P. Kremsdorf D. Weiss M.C. Proc. Natl. Acad. Sci. U. S. A. 2004; 101: 8360-8365Crossref PubMed Scopus (105) Google Scholar). The cells were transfected, using LipofectAMINE 2000, in 6-well plates for 24 h with 6 μg of the CMV promoter-driven pcDNA3-EGFP (Invitrogen), pCMV-Flag/HNF-6, or pCMV-Flag/HNF-6-VP16 expression vectors. To perform chromatin immunoprecipitation cells were transfected in 10-cm dishes for 26 h with 15 μg of the pCMV-Flag/HNF-6 expression vector. Protein Extraction and Western Blotting—Protein extraction was performed according to the manufacturer's instructions on the same samples from which RNA was isolated with the Tripure reagent. Equal volumes were loaded on SDS-PAGE gels, as protein concentration could not be measured. Western blotting was performed as described (21Pierreux C.E. Nicolas F.J. Hill C.S. Mol. Cell. Biol. 2000; 20: 9041-9054Crossref PubMed Scopus (228) Google Scholar) with anti-GFP (Molecular Probes) or anti-FLAG (M2, Sigma) antibodies. Chromatin Immunoprecipitation—This was performed essentially as described (22Nowling T. Bernadt C. Johnson L. Desler M. Rizzino A. J. Biol. Chem. 2003; 278: 13696-13705Abstract Full Text Full Text PDF PubMed Scopus (34) Google Scholar) on 107 BMEL cells transfected with the Flag/HNF-6 expression vector. DNA-protein complexes were cross-linked by addition of formaldehyde to the culture medium (final concentration 1%). After 10 min at room temperature, the cross-linking reaction was stopped by addition of glycine (final concentration 0.125 m) for 5 min. Cells were washed and harvested by scraping into phosphate-buffered saline containing 0.5 mm phenylmethylsulfonyl fluoride. The cells were lyzed in a buffer containing 10 mm Tris-Cl, pH 8.0, 85 mm KCl, 0.5% IGEPAL CA-630, 0.5 mm phenylmethylsulfonyl fluoride and protease inhibitors, and nuclei were collected. Nuclei were lyzed in a buffer containing 50 mm Tris-Cl, pH 8.0, 10 mm EDTA, 1% SDS, 0.5 mm phenylmethylsulfonyl fluoride and protease inhibitors, and were sonicated to produce DNA fragments of ∼200 to 1000 bp in length. The samples were centrifuged to eliminate cell debris, and DNA-protein complexes were precleared with salmon sperm DNA/protein A-agarose (Upstate) for 15 min at 4 °C with rotation. Each sample was then diluted with 0.01% SDS, 1% Triton X-100, 1.2 mm EDTA, 16.7 mm Tris-Cl, pH 8.0, 167 mm NaCl, an aliquot was removed as “input” control, and the remaining sample was divided into two aliquots. M2 (anti-FLAG; Sigma) antibody was added to one aliquot, and anti-tubulin (Sigma) antibody was added to the second aliquot as a nonspecific antibody control. Samples were incubated with antibody overnight with rotation at 4 °C. The following day, salmon sperm DNA/protein A-agarose beads were added to the samples and incubated for 1–2 h at 4 °C with rotation to collect DNA-protein-antibody complexes. Beads were washed and DNA-protein complexes were eluted by two successive incubations at 65 °C for 10 min with freshly prepared elution buffer (1% SDS, 50 mm NaHCO3). NaCl (final concentration, 0.3 m) and RNase A were added to the eluted and input samples and incubated at 65 °C for 4–6 h to revert cross-links. Samples were precipitated overnight at -20 °C by addition of 2.5 volumes of 100% ethanol, resuspended in proteinase K buffer (10 mm Tris-Cl, pH 7.5, 5 mm EDTA, 0.25% SDS), and treated with proteinase K for 2 h at 45 °C. After this, DNA was purified by phenol/chloroform and chloroform extraction followed by ethanol precipitation. PCR was performed on 1/50th of the immunoprecipitated chromatin. Whole Embryo Culture and Electroporation—Whole embryo culture and electroporation have been described in detail. 2C. E. Pierreux, A. V. Poll, P. Jacquemin, F. P. Lemaigre, and G. G. Rousseau, submitted for publication. Briefly, intact e8.5 embryos were incubated in a DNA solution and placed between the electrodes. Electroporation was performed using a square-wave pulse generator (Electro Square Porator ECM 830, BTX). After electroporation, embryos were cultured for 24 h at 37 °C in a roller culture system (31 rpm). Electroporated embryos developed normally and were further dissected for RNA extraction or processed for paraffin embedding, as for normal e9.5 embryos. Hnf6, Oc2, and Oc3 Are Expressed Sequentially in the Endoderm—Work from our laboratory has shown that HNF-6 is expressed in the mouse endoderm starting around the 8-somite stage (e8.5) (14Jacquemin P. Lemaigre F.P. Rousseau G.G. Dev. Biol. 2003; 258: 105-116Crossref PubMed Scopus (157) Google Scholar). In situ hybridization experiments have revealed expression of Oc2 in the endoderm of 14-somite (e9) embryos (13Jacquemin P. Pierreux C.E. Fierens S. van Eyll J.M. Lemaigre F.P. Rousseau G.G. Gene Expr. Patterns. 2003; 3: 639-644Crossref PubMed Scopus (40) Google Scholar). No data are available on Oc-3 expression in the endoderm. To determine the level of expression of the three Onecut factors in the endoderm, we resorted to real time PCR experiments on RNA extracted from endoderm isolated by dispase treatment of the embryos at the 5-somite to the 14-somite stage. We also measured Pdx-1 mRNA as an endodermal marker. As shown in Table I, the three Onecut factors were expressed in the endoderm. HNF-6 mRNA was the most abundant and was clearly detectable at the 5-somite stage. The onset of Oc2 expression was delayed as compared with that of Hnf6, being detectable at the 7-somite stage. Oc3 expression became significant only at the 14-somite stage. On e9.5, expression of Oc3 had increased to the point of reaching about 25% of that of Hnf6 at that stage (Fig. 1A). We conclude from these data that the three Onecut factors become expressed sequentially in the developing endoderm, in the order HNF-6, OC-2, OC-3.Table IExpression of transcription factors in the developing endoderm Endoderm was dissected from mouse embryos at the somitic stages indicated and the RNA was extracted. Real time PCR analysis for the transcription factors listed was performed on the cDNAs. The results, from individual embryos (including two at the 9-somite stage) are expressed as (transcription factor mRNA copy number/actin mRNA copy number) × 106.SomitesTranscription factor mRNAHNF-6OC-2OC-3Pdx-1537NDaND, not detectableND4681NDND10712795ND48917641ND5216909ND93142407212128a ND, not detectable Open table in a new tab Oc3 Expression Is Down-regulated in Hnf6 Knock-out Embryos—As HNF-6 is the first Onecut factor to be expressed in the endoderm, we investigated whether it could regulate the expression of the two other Onecut factors. To this end, we examined the consequences of inactivating the Hnf6 gene. We compared control and Hnf6 knock-out embryos and determined the expression of Oc2 and Oc3 at e9.5 (22 somites) in the primitive gut region that will give rise to the liver, pancreas, stomach, and duodenum, and which corresponds to the HNF-6 expression territory. Whereas the expression of Oc2 was unaffected in the absence of HNF-6, the expression of Oc3 was undetectable as compared with controls (Fig. 1A). Expression of Oc3 in the primitive gut of Hnf6 knock-out embryos was also abolished at e10.5 (35 somites, Fig. 1B) and this persisted in the pancreas and liver at e12.5 (Fig. 1, C and D). We conclude that HNF-6 controls Oc3 gene expression in the endoderm and the developing pancreas and liver. OC-3 Is Colocalized with HNF-6—As a first step to investigate whether HNF6 exerts a direct control on Oc3 gene expression, we verified if the two factors are coexpressed. Sections from 19-somite embryos were examined by immunofluorescence using antibodies directed against HNF-6 and OC-3. To localize the endodermal epithelium and to distinguish it from the surrounding nonepithelial tissues, we used an antibody against E-cadherin, which is a component of the adherens junction complex between epithelial cells. As shown in Fig. 2A, HNF-6 nuclear staining was observed in all the endodermal cells in this region. Two adjacent sections stained with the anti-OC-3 antibody revealed nuclear OC-3 expression in cells on the ventral side of the endoderm (Fig. 2, B and C). As nuclei in this region were also positive for HNF-6 (Fig. 2A), we conclude that part of the cell population that expresses HNF-6 also expresses OC-3. To identify the cell types that express OC-3, these sections were costained with antibodies directed against Pdx-1, which labels pancreatic precursors (Fig. 2B), and Prox1, which labels hepatic precursors (23Burke Z. Oliver G. Mech. Dev. 2002; 118: 147-155Crossref PubMed Scopus (123) Google Scholar) (Fig. 2C). OC-3 was widely expressed in part of the ventral prepancreatic region as well as in the prehepatic region. At this stage, OC-3 was not detected in other regions of the endoderm. Thus, around e9.5, OC-3 is expressed in the ventral prepancreatic and prehepatic regions, a territory of the endoderm that also express HNF-6. One day later (32-somite stage), we performed immunohistochemistry for HNF-6 and OC-3, to better identify the developing organs in which they are expressed. HNF-6 protein was detected in the migrating hepatoblasts, gallbladder primordium, ventral and dorsal pancreas, stomach, and duodenum (Fig. 2D). OC-3 protein colocalized with HNF-6 in the migrating hepatoblasts, gallbladder primordium, ventral and dorsal pancreas as well as in some cells of the stomach and duodenum (Fig. 2E). This indicates that OC-3 is expressed in discrete regions of the endoderm were HNF-6 is present. HNF-6 Binds to and Stimulates Transcription of the Oc3 Gene—To determine whether HNF-6 can bind to, and directly stimulate, the transcription of the Oc3 gene, we resorted to cultures of BMEL cells isolated from e14.5 liver as described in Strick-Marchand and colleagues (19Strick-Marchand H. Weiss M.C. Hepatology. 2002; 36: 794-804Crossref PubMed Google Scholar, 20Strick-Marchand H. Moresan S. Charneau P. Kremsdorf D. Weiss M.C. Proc. Natl. Acad. Sci. U. S. A. 2004; 101: 8360-8365Crossref PubMed Scopus (105) Google Scholar). These cells have properties of hepatoblasts, as they can differentiate toward the hepatocytic or biliary lineages (19Strick-Marchand H. Weiss M.C. Hepatology. 2002; 36: 794-804Crossref PubMed Google Scholar, 20Strick-Marchand H. Moresan S. Charneau P. Kremsdorf D. Weiss M.C. Proc. Natl. Acad. Sci. U. S. A. 2004; 101: 8360-8365Crossref PubMed Scopus (105) Google Scholar). They express the three Onecut transcription factors and can be transfected with high (close to 80%) efficiency. Moreover, we found that Hnf6 knock-out BMEL cells express five times less Oc3 than wild type cells (data not shown). We first examined if HNF-6 could stimulate endogenous Oc3 gene transcription. Cells were transfected with a GFP expression vector, as a control, or with Flag/HNF-6 or Flag/HNF-6/VP16 expression vectors. Total RNA was extracted 24 h later and reverse transcribed to measure endogenous Oc2 and Oc3 expression levels by real time PCR (Fig. 3A). Oc2 and Oc3 expression was the same in cells transfected with the GFP expression vector as in nontransfected cells (data not shown). Expression of GFP, Flag/HNF-6, and Flag/HNF-6/VP16 in the cells was assessed by Western blotting (Fig. 3B). Transfection of the expression vector coding for HNF-6 did not influence Oc2 gene expression (Fig. 3A, top panel). In contrast, the expression levels of Oc3 in the cells transfected with HNF-6 were about five times those in the control (GFP-transfected) cells and even higher when a HNF-6/VP16 fusion protein was expressed in the cells (Fig. 3A, bottom panel). This indicates that HNF-6 can specifically stimulate transcription of the Oc3 gene, without affecting transcription in general. We then searched for HNF-6 binding sites in regulatory regions of the Oc3 gene. Comparison of the human and mouse genes revealed sequence conservation over about 1 kb upstream (-2880 to -1950 from the ATG) of the coding sequence. This region contains three conserved HNF-6 binding consensus sequences (Fig. 3C). To test if HNF-6 binds to the Oc3 gene, we performed chromatin immunoprecipitation experiments. The chromatin from Flag/HNF-6-transfected BMEL cells was immunoprecipitated using an anti-FLAG antibody and the immunoprecipitated fraction was tested by PCR for the presence of Oc3 gene regions containing the HNF-6 binding sites. As shown in Fig. 3C, the three regions were amplified from chromatin that had been immunoprecipitated with the anti-FLAG antibody. No amplification was observed with an unrelated antibody. Altogether, these results indicate that HNF-6 binds to a conserved region of the Oc3 gene in native chromatin and stimulates transcription of this gene. HNF-6 Induces Oc3 Gene Expression in the Developing Endoderm—We then designed experiments to confirm that HNF-6 can stimulate Oc3 gene transcription in the mouse endoderm. We recently developed a system that allows delivery of DNA to discrete regions of the mouse endoderm in situ such as the prepancreatic or prehepatic territories.2 Electroporation of precisely oriented 6- to 8-somite embryos is followed by a 24-h culture of the whole embryos such that they reach the 18–22-somite stage (Fig. 4A). Morphogenic events occur as they do in vivo during that period and the sheet of cells that forms the endoderm gives rise to the primitive gut. Using this technique we delivered a HNF-6 expression vector in the foregut-midgut region of the endoderm of e8.5 embryos, together with a GFP expression vector to assess electroporation efficiency. Nonelectroporated embryos were cultured in parallel as controls. After the 24-h culture, the region of the primitive gut corresponding to the targeted region was dissected out and total RNA was extracted and reverse transcribed. Fig. 4B shows that the electroporated embryos expressed Gfp and that Hnf6 was expressed in the endoderm electroporated with the HNF-6 expression vector. Expression levels of the endogenous Onecut genes in the endoderm of these embryos were measured by real time PCR. Endogenous Hnf6 mRNA was detected by using an antisense primer in the 3′-untranslated region of Hnf6, which is not present in the HNF-6 expression vector. As shown in Fig. 4C, the levels of Onecut factor mRNAs were not influenced by the electroporation per se. Expression of exogenous HNF-6 had no effect on Hnf6 or Oc2 expression. In contrast, it increased dramatically Oc3 expression in the endoderm. To demonstrate that Oc3 is induced in the cells transfected with HNF-6, we cultured embryos for 24 h after electroporation with the HNF-6 expression vector and performed immunofluorescence on sections costained for HNF-6 and E-cadherin to localize the endodermal epithelium (Fig. 5A). A cluster of cells in the gut endoderm reacted with the anti-HNF-6 antibody. Note that this HNF-6 staining is so strong that the endogenous HNF-6, albeit present, is barely visible. The electroporated cells were localized in the prehepatic region as revealed by the Prox1 immunoreactivity (Fig. 5B). The adjacent section, stained with anti-OC-3 and anti-Pdx-1 antibodies, revealed strong expression of OC-3 in the cluster of HNF-6 positive cells opposite the Pdx-1 territory (Fig. 5C), supporting the idea that HNF-6 directly induces OC-3 expression. We conclude that HNF6 cell-autonomously stimulates OC3 expression in the endoderm. We next addressed the question as to whether HNF-6 can induce Oc3 expression in cells that are not fated to normally express OC-3. By orientating the embryo between the electrodes so that DNA is delivered in the anterior part of the foregut pocket, we targeted the esophageal region (boxed in Fig. 5D), as demonstrated by the expression of HNF-6 in some cells of this region (Fig. 5E). We observed cells that were positive for OC-3 in this electroporated esophageal region (Fig. 5F), indicating that HNF-6 is able to turn on the transcription of the Oc3 gene ectopically. Development of the liver and pancreas from the endoderm involves the hepatic or pancreatic commitment of endodermal precursors, followed by differentiation of these precursors into the corresponding tissue-specific cell types. These processes depend on a network of transcription factors, whose onset of expression and mutual control is far from being completely elucidated. Our earlier work has demonstrated that HNF-6 is an important member of this network, by showing that inactivation of the Hnf6 gene perturbs liver and pancreas development and by identifying transcription factors as HNF-6 target genes. Thus, in the mouse embryo, HNF-6 stimulates Hnf1β expression in liver, thereby controlling intrahepatic bile duct morphogenesis (16Clotman F. Lannoy V.J. Reber M. Cereghini S. Cassiman D. Jacquemin P. Roskams T. Rousseau G.G. Lemaigre F.P. Development. 2002; 129: 1819-1828Crossref PubMed Google Scholar), and in the pancreas HNF-6 stimulates expression of Hnf1β (24Maestro M.A. Boj S.J. Luco R.F. Pierreux C.E. Cabedo J. Servitja J.M. German M.S. Rousseau G.G. Lemaigre F.P. Ferrer J. Hum. Mol. Genet. 2004; 12: 3307-3314Crossref Scopus (122) Google Scholar) and Ngn3 (15Jacquemin P. Durviaux S.M. Jensen J. Godfraind C. Gradwohl G. Guillemot F. Madsen O.D. Carmeliet P. Dewerchin M. Collen D. Rousseau G.G. Lemaigre F.P. Mol. Cell. Biol. 2000; 20: 4445-4454Crossref PubMed Scopus (277) Google Scholar), thereby controlling endocrine differentiation. HNF-6 also plays a role at an earlier stage in the endoderm where it is involved in the onset of Pdx1 expression (14Jacquemin P. Lemaigre F.P. Rousseau G.G. Dev. Biol. 2003; 258: 105-116Crossref PubMed Scopus (157) Google Scholar). In this paper, we identify a second transcription factor as an HNF-6 target in developing endoderm, namely its paralog OC-3. Indeed, we show here that HNF-6 can bind to the Oc3 gene in native chromatin and that it can stimulate Oc3 expression not only in transfected cells, but also in the endoderm in situ. Moreover, this regulatory cascade is developmentally relevant, as inactivation of the Hnf6 gene leads to a down-regulation of OC-3 both in the endoderm and in the endoderm-derived organs that express HNF-6, namely the liver and pancreas. It is noteworthy that induction of Oc3 expression by HNF-6 is limited in time and space. At e9, expression of OC3 in the endoderm is detected only in a subpopulation of HNF-6-expressing cells, whereas at e10.5 OC-3 expression extends to almost all the HNF-6 territory, which corresponds to the liver, pancreas, and part of the stomach and gut. Later on, HNF-6 becomes restricted to the liver and the pancreas, whereas OC-3 disappears from these tissues but remains expressed in the stomach and in the intestine (10Vanhorenbeeck V. Jacquemin P. Lemaigre F.P. Rousseau G.G. Biochem. Biophys. Res. Commun. 2002; 292: 848-854Crossref PubMed Scopus (34) Google Scholar, 11Landry C. Clotman F. Hioki T. Oda H. Picard J.J Lemaigre F.P. Rousseau G.G. Dev. Biol. 1997; 192: 247-257Crossref PubMed Scopus (139) Google Scholar, 12Rausa F. Samadani U. Ye H. Lim L. Fletcher C.F. Jenkins N.A. Copeland N.G. Costa R.H. Dev. Biol. 1997; 192: 228-246Crossref PubMed Scopus (157) Google Scholar). The induction of Oc3 by HNF-6 may require time and space-restricted cooperation of HNF-6 with other Oc3 regulatory factors and/or posttranslational regulation of HNF-6 activity (25Streeper R.S. Hornbuckle L.A. Svitek C.A. Goldman J.K. Oeser J.K. O'Brien R.M. J. Biol. Chem. 2001; 276: 19111-19118Abstract Full Text Full Text PDF PubMed Scopus (32) Google Scholar). We also show here that all three Onecut factors are expressed in a region of the endoderm that will eventually form the stomach, duodenum, liver, and pancreas. Interestingly, they are expressed in the sequential order Hnf6, Oc2, Oc3, so that Onecut factor expression defines three successive steps in endoderm maturation (Fig. 6A). This is relevant for understanding pancreas and liver development and for implementing this information in the context of cell therapy. Indeed, transcription factors alone or in combination are used to promote controlled cell differentiation and are useful markers to determine the precise differentiation stages of cells that are cultured in well defined conditions. Our gene expression analysis of Hnf6 knock-out embryos extends our knowledge of the transcription factor network in the endoderm (Fig. 6B). First, OC-2 induction is independent from HNF-6 because we show here that OC-2 is expressed in the Hnf6 knock-out embryos and that electroporation of HNF-6 in cultured cells or in the endoderm does not induce Oc2. Second, our data would rule out that it is OC-2 that triggers Oc3 gene transcription in the endoderm. Indeed, in the Hnf6 knock-out, OC-3 is not expressed despite the normal expression of OC-2. Moreover, ectopic expression of HNF-6 in the esophagus induced Oc3 expression, in the absence of OC-2. Another implication of the present work bears on the interpretation of the endodermal phenotype of Hnf6 knock-out embryos. Pdx-1 expression in their endoderm is retarded from the 8–12-somite stage until the 19–22-somite stage (14Jacquemin P. Lemaigre F.P. Rousseau G.G. Dev. Biol. 2003; 258: 105-116Crossref PubMed Scopus (157) Google Scholar), i.e. Pdx-1 appears at the time when OC-3 becomes significantly expressed in the normal embryo. This could have suggested that it is OC-3 that triggers Pdx-1 expression in the absence of HNF-6. Our data show that this is unlikely, as in the Hnf6 knock-out Pdx-1 eventually appears despite the absence of OC-3. The question of the possible role of OC-2 in inducing Pdx-1 in the Hnf6 knock-out embryos remains open, because OC-2 levels are unaffected in these knock-out embryos. On the other hand, the fact that OC-3 is down-regulated in the Hnf6 knock-out embryos may account for some developmental defects of these embryos. These defects might result from failed expression of genes that are targeted specifically by OC-3 alone or by both HNF-6 and OC-3. Because, in normal embryos, OC-3 is first detected on the ventral side (prehepatic and prepancreatic territories) of the endoderm, one would expect that the consequences of this combined HNF-6/OC-3 deficiency appear first in the ventral prepancreatic and/or prehepatic endoderm. This is indeed the case. In Hnf6 knock-out embryos, development is more severely affected on the ventral side than on the dorsal side, with an absence of gallbladder, a longer delay in the expression of Pdx-1, and an extremely reduced ventral pancreatic bud (14Jacquemin P. Lemaigre F.P. Rousseau G.G. Dev. Biol. 2003; 258: 105-116Crossref PubMed Scopus (157) Google Scholar, 16Clotman F. Lannoy V.J. Reber M. Cereghini S. Cassiman D. Jacquemin P. Roskams T. Rousseau G.G. Lemaigre F.P. Development. 2002; 129: 1819-1828Crossref PubMed Google Scholar). Earlier experiments with cultured cells and on Hnf6 knock-out embryos have shown that HNF-6 can bind to and stimulate several genes coding for transcription factors. Thus, HNF-6 participates to the networks of transcription factors that regulate development of the endoderm and endoderm-derived organs. These factors include HNF-3β, HNF-4, HNF-1β, Pdx-1, and Ngn3. The present work extends our knowledge of the control exerted on these factors, not only by HNF-6, but also by the other Onecut proteins (Fig. 6B). In the endoderm, HNF-6 is required for normal expression of Pdx1 (14Jacquemin P. Lemaigre F.P. Rousseau G.G. Dev. Biol. 2003; 258: 105-116Crossref PubMed Scopus (157) Google Scholar), Oc3 (this paper), and Hnf1β, 3C. E. Pierreux, F. P. Lemaigre, and G. G. Rousseau, unpublished observations. but not for that of Hnf3β (14Jacquemin P. Lemaigre F.P. Rousseau G.G. Dev. Biol. 2003; 258: 105-116Crossref PubMed Scopus (157) Google Scholar) or Hnf4. In the liver, HNF-6 is critical for expression of Oc3 (this paper) and Hnf1β, and this HNF-6 → Hnf1β cascade controls bile duct development (16Clotman F. Lannoy V.J. Reber M. Cereghini S. Cassiman D. Jacquemin P. Roskams T. Rousseau G.G. Lemaigre F.P. Development. 2002; 129: 1819-1828Crossref PubMed Google Scholar). Whereas independent inactivation of Hnf6 or Oc2 does not affect Hnf4 expression in liver, the combined inactivation of Hnf6 and Oc2 strongly reduces expression of the α7 isoform of HNF-4 (26Briançon N. Bailly A. Clotman F. Jacquemin P. Lemaigre F.P. Weiss M.C. J. Biol. Chem. 2004; 279: 33398-33408Abstract Full Text Full Text PDF PubMed Scopus (58) Google Scholar). The expression of Hnf3α is also controlled by HNF-6 in the liver, but via an inhibitory, transforming growth factor β-dependent mechanism (27Plumb-Rudewiez N. Clotman F. Strick-Marchand H. Pierreux C.E. Weiss M.C. Rousseau G.G. Lemaigre F.P. Hepatology. 2004; (in press)PubMed Google Scholar). In the pancreas, HNF-6 is required for Oc3 expression (this paper) and HNF-6 controls Hnf1β (24Maestro M.A. Boj S.J. Luco R.F. Pierreux C.E. Cabedo J. Servitja J.M. German M.S. Rousseau G.G. Lemaigre F.P. Ferrer J. Hum. Mol. Genet. 2004; 12: 3307-3314Crossref Scopus (122) Google Scholar) and Ngn3 (15Jacquemin P. Durviaux S.M. Jensen J. Godfraind C. Gradwohl G. Guillemot F. Madsen O.D. Carmeliet P. Dewerchin M. Collen D. Rousseau G.G. Lemaigre F.P. Mol. Cell. Biol. 2000; 20: 4445-4454Crossref PubMed Scopus (277) Google Scholar) expression during endocrine differentiation. Although no OC-3 target genes have been identified to date, this factor may be involved in the differentiation of the endoderm and endoderm-derived tissues, downstream of HNF-6. In conclusion, the data presented here broaden our understanding of the network of transcription factors that are expressed in the endoderm. They define stages in the embryonic expression pattern of the Onecut transcription factors. They identify OC-3 as a new target of HNF-6 in the endoderm as well as in developing pancreas and liver, pointing to a new mechanism by which HNF-6 can control developmental events. We thank Sabine Cordi for excellent technical assistance, Jean-François Cornut for helping in the preparation of OC-3 antibodies, Chris V. Wright for a generous gift of anti-Pdx-1 antibody, and members of the HORM unit for discussion." @default.
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• W2074305938 title "The Transcription Factor Hepatocyte Nuclear Factor-6/Onecut-1 Controls the Expression of Its Paralog Onecut-3 in Developing Mouse Endoderm" @default.
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• W2074305938 cites W2015611354 @default.
• W2074305938 cites W2015757949 @default.
• W2074305938 cites W2015922342 @default.
• W2074305938 cites W2025397278 @default.
• W2074305938 cites W2038072610 @default.
• W2074305938 cites W2068190950 @default.
• W2074305938 cites W2077041310 @default.
• W2074305938 cites W2080999400 @default.
• W2074305938 cites W2082073317 @default.
• W2074305938 cites W2085502970 @default.
• W2074305938 cites W2089328941 @default.
• W2074305938 cites W2092281396 @default.
• W2074305938 cites W2092922929 @default.
• W2074305938 cites W2094432949 @default.
• W2074305938 cites W2095475285 @default.
• W2074305938 cites W2119546366 @default.
• W2074305938 cites W2143973293 @default.
• W2074305938 cites W2147769151 @default.
• W2074305938 cites W2151382751 @default.
• W2074305938 cites W2162822589 @default.
• W2074305938 cites W2164439333 @default.
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