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- W2074327462 abstract "A lactic acid biosensor was prepared with lactate oxidase (LOD) from Pediococcus species, which was immobilized in a polyion membrane containing poly-L-lysine and poly(4-styrenesulfonate). Bifunctional poly(ethyleneglycol)(400)diglycidyl ether cross-linked the polyion to a glassy carbon (GC) electrode surface modified with cobalt phthalocyanine layer. Hydrogen peroxide produced by the reaction of lactate and LOD was detected on the cobalt phthalocyanine-modified GC electrode operated at a potential of +600 mV (vs. Ag/AgCl). The response time of the sensor to 10 μmol dm–3lactate was 1 s, and it required 45 s to give a 100% steady-state response of ca. 10 nA. The detection limit estimated from signal to noise ratio was 0.5 μmol dm–3. The LOD/polyion biosensor exhibited electrochemical Michaelis-Menten kinetics and gave an average apparent Michaelis-Menten constant,K′M, value of 0.88 ± (0.07 mmol dm–3over a storage period of 18 days. Using lower molecular weight poly-L-lysine, resulted in 10 times decrease in ascorbic acid interference in lactate sensing. The polyion matrix can be constructed to be an ultrafiltration membrane to exclude electroactive compounds that interfere with lactic acid determinations in analytical samples." @default.
- W2074327462 created "2016-06-24" @default.
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- W2074327462 date "1999-05-01" @default.
- W2074327462 modified "2023-09-24" @default.
- W2074327462 title "AmperometricL-Lactate Biosensors: 1. Lactic Acid Sensing Electrode Containing Lactate Oxidase in a Composite Poly-L-lysine Matrix" @default.
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- W2074327462 doi "https://doi.org/10.1002/(sici)1521-4109(199905)11:5<367::aid-elan367>3.0.co;2-0" @default.
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