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- W2074397789 abstract "A device for continuous differential impedance analysis of single cells held by a hydrodynamic cell trapping is presented. Measurements are accomplished by recording the current from two closely-situated electrode pairs, one empty (reference) and one containing a cell. We demonstrate time-dependent measurement of single cell impedance produced in response to dynamic chemical perturbations. First, the system is used to assay the response of HeLa cells to the effects of the surfactant Tween, which reduces the impedance of the trapped cells in a concentration dependent way and is interpreted as gradual lysis of the cell membrane. Second, the effects of the bacterial pore-forming toxin, Streptolysin-O are measured: a transient exponential decay in the impedance is recorded as the cell membrane becomes increasingly permeable. The decay time constant is inversely proportional to toxin concentration (482, 150, and 30 s for 0.1, 1, and 10 kU/ml, respectively)." @default.
- W2074397789 created "2016-06-24" @default.
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- W2074397789 date "2009-12-10" @default.
- W2074397789 modified "2023-10-02" @default.
- W2074397789 title "Continuous differential impedance spectroscopy of single cells" @default.
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- W2074397789 doi "https://doi.org/10.1007/s10404-009-0534-2" @default.
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