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- W2074408087 abstract "A simple, rapid, fairly pure preparation of dog antithrombin III (AT) is obtained by two passages through heparin-Sepharose. The AT is labelled with 131Iodine using lactoperoxidase, and unbound 131I and other contaminants are removed on a third heparin-Sepharose column. SDS-PAGE and isoelectric focussing show negligible differences between AT and 131I-AT while measurements of heparin cofactor and progressive antithrombin activities show at most loss of 5% activity by the 131I-AT. Comparisons of turnover behavior of “unscreened” and “screened” 131I-AT in dogs showed no significant differences. However, predictions from the plasma data using the standard kinetic model of plasma 131I-protein failed to fit the observed whole body 131I behavior. Fractional breakdown rate averaged .51, SD .056, and there was no difference between males and females. The approximate ratio of interstitial/plasma AT was 1.0, SD .13." @default.
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- W2074408087 date "1980-11-01" @default.
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- W2074408087 title "Studies with 131I-labelled antithrombin III in dogs" @default.
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- W2074408087 doi "https://doi.org/10.1016/0049-3848(80)90277-7" @default.
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