FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2074439911> ?p ?o ?g. }

• W2074439911 endingPage "e81792" @default.
• W2074439911 startingPage "e81792" @default.
• W2074439911 abstract "Herein we describe a pathogenic role for the Pseudomonas aeruginosa type three secretion system (T3SS) needle tip complex protein, PcrV, in causing lung endothelial injury. We first established a model in which P. aeruginosa wild type strain PA103 caused pneumonia-induced sepsis and distal organ dysfunction. Interestingly, a PA103 derivative strain lacking its two known secreted effectors, ExoU and ExoT [denoted PA103 (ΔU/ΔT)], also caused sepsis and modest distal organ injury whereas an isogenic PA103 strain lacking the T3SS needle tip complex assembly protein [denoted PA103 (ΔPcrV)] did not. PA103 (ΔU/ΔT) infection caused neutrophil influx into the lung parenchyma, lung endothelial injury, and distal organ injury (reminiscent of sepsis). In contrast, PA103 (ΔPcrV) infection caused nominal neutrophil infiltration and lung endothelial injury, but no distal organ injury. We further examined pathogenic mechanisms of the T3SS needle tip complex using cultured rat pulmonary microvascular endothelial cells (PMVECs) and revealed a two-phase, temporal nature of infection. At 5-hours post-inoculation (early phase infection), PA103 (ΔU/ΔT) elicited PMVEC barrier disruption via perturbation of the actin cytoskeleton and did so in a cell death-independent manner. Conversely, PA103 (ΔPcrV) infection did not elicit early phase PMVEC barrier disruption. At 24-hours post-inoculation (late phase infection), PA103 (ΔU/ΔT) induced PMVEC damage and death that displayed an apoptotic component. Although PA103 (ΔPcrV) infection induced late phase PMVEC damage and death, it did so to an attenuated extent. The PA103 (ΔU/ΔT) and PA103 (ΔPcrV) mutants grew at similar rates and were able to adhere equally to PMVECs post-inoculation indicating that the observed differences in damage and barrier disruption are likely attributable to T3SS needle tip complex-mediated pathogenic differences post host cell attachment. Together, these infection data suggest that the T3SS needle tip complex and/or another undefined secreted effector(s) are important determinants of P. aeruginosa pneumonia-induced lung endothelial barrier disruption." @default.
• W2074439911 created "2016-06-24" @default.
• W2074439911 creator A5014920660 @default.
• W2074439911 creator A5018076381 @default.
• W2074439911 creator A5019627368 @default.
• W2074439911 creator A5026097992 @default.
• W2074439911 creator A5033132770 @default.
• W2074439911 creator A5037045639 @default.
• W2074439911 creator A5043335237 @default.
• W2074439911 creator A5047035334 @default.
• W2074439911 creator A5050459313 @default.
• W2074439911 creator A5052920879 @default.
• W2074439911 creator A5063231602 @default.
• W2074439911 date "2013-11-27" @default.
• W2074439911 modified "2023-09-27" @default.
• W2074439911 title "In the Absence of Effector Proteins, the Pseudomonas aeruginosa Type Three Secretion System Needle Tip Complex Contributes to Lung Injury and Systemic Inflammatory Responses" @default.
• W2074439911 cites W1511786918 @default.
• W2074439911 cites W1539422958 @default.
• W2074439911 cites W1596372166 @default.
• W2074439911 cites W1732205261 @default.
• W2074439911 cites W1821732051 @default.
• W2074439911 cites W1855520672 @default.
• W2074439911 cites W191624541 @default.
• W2074439911 cites W1916325998 @default.
• W2074439911 cites W1978839325 @default.
• W2074439911 cites W1984855296 @default.
• W2074439911 cites W1986731647 @default.
• W2074439911 cites W1986764080 @default.
• W2074439911 cites W1988513409 @default.
• W2074439911 cites W1991794624 @default.
• W2074439911 cites W1991855809 @default.
• W2074439911 cites W2003961381 @default.
• W2074439911 cites W2004668209 @default.
• W2074439911 cites W2004677549 @default.
• W2074439911 cites W2005433255 @default.
• W2074439911 cites W2007678223 @default.
• W2074439911 cites W2015853335 @default.
• W2074439911 cites W2016189603 @default.
• W2074439911 cites W2018079325 @default.
• W2074439911 cites W2018652833 @default.
• W2074439911 cites W2019543594 @default.
• W2074439911 cites W2020683003 @default.
• W2074439911 cites W2021071631 @default.
• W2074439911 cites W2022407885 @default.
• W2074439911 cites W2030141364 @default.
• W2074439911 cites W2031567708 @default.
• W2074439911 cites W2046877592 @default.
• W2074439911 cites W2052874989 @default.
• W2074439911 cites W2056085992 @default.
• W2074439911 cites W2056926111 @default.
• W2074439911 cites W2059922187 @default.
• W2074439911 cites W2062106725 @default.
• W2074439911 cites W2077049678 @default.
• W2074439911 cites W2082264097 @default.
• W2074439911 cites W2083364047 @default.
• W2074439911 cites W2083652732 @default.
• W2074439911 cites W2084663415 @default.
• W2074439911 cites W2086724299 @default.
• W2074439911 cites W2087967484 @default.
• W2074439911 cites W2096797367 @default.
• W2074439911 cites W2097663027 @default.
• W2074439911 cites W2097948871 @default.
• W2074439911 cites W2098800458 @default.
• W2074439911 cites W2098871654 @default.
• W2074439911 cites W2101002582 @default.
• W2074439911 cites W2104346094 @default.
• W2074439911 cites W2105593245 @default.
• W2074439911 cites W2112583566 @default.
• W2074439911 cites W2113960845 @default.
• W2074439911 cites W2115574740 @default.
• W2074439911 cites W2119236186 @default.
• W2074439911 cites W2121138686 @default.
• W2074439911 cites W2125766883 @default.
• W2074439911 cites W2126597145 @default.
• W2074439911 cites W2137519179 @default.
• W2074439911 cites W2140167462 @default.
• W2074439911 cites W2141350298 @default.
• W2074439911 cites W2142783037 @default.
• W2074439911 cites W2144407253 @default.
• W2074439911 cites W2147891283 @default.
• W2074439911 cites W2150408547 @default.
• W2074439911 cites W2152344036 @default.
• W2074439911 cites W2158102238 @default.
• W2074439911 cites W2158851958 @default.
• W2074439911 cites W2161014327 @default.
• W2074439911 cites W2164716310 @default.
• W2074439911 cites W2321428848 @default.
• W2074439911 cites W2404208636 @default.
• W2074439911 cites W2531609094 @default.
• W2074439911 cites W2912349604 @default.
• W2074439911 cites W4294658238 @default.
• W2074439911 doi "https://doi.org/10.1371/journal.pone.0081792" @default.
• W2074439911 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3842252" @default.
• W2074439911 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/24312357" @default.
• W2074439911 hasPublicationYear "2013" @default.
• W2074439911 type Work @default.
• W2074439911 sameAs 2074439911 @default.
• W2074439911 citedByCount "23" @default.

• next