FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2074443822> ?p ?o ?g. }

• W2074443822 endingPage "731" @default.
• W2074443822 startingPage "725" @default.
• W2074443822 abstract "HIV-1 Nef is an accessory protein necessary for HIV-1 virulence and rapid AIDS development. Nef promotes viral replication and infection by connecting CD4 and several other cell surface receptors to the clathrin adaptor protein AP2, resulting in the internalization and degradation of the receptors interacting with Nef. We investigated how Nef can mediate constitutive receptor endocytosis through the interaction of the dileucine motif in its C-terminal flexible loop (C-loop) with AP2, whereas AP2 binding of the transmembrane receptors usually results in an equilibrated (recycled) endocytosis. Our results indicated that in addition to the dileucine motif, there is a second motif in the Nef C-loop involved in the Nef-AP2 interaction. Nef-mediated CD4 downregulation was impaired when the residue in the hydrophobic region in the Nef C-loop (LL165HPMSLHGM173) was mutated to a basic residue K/R or an acidic residue E/D or to the rigid residue P, or when M168L170, L170H171, or G172M173 was mutated to AA. A pull-down assay indicated that AP2 was not coprecipitated with Nef mutants that did not downregulate CD4. Molecular modeling of the Nef C-terminal flexible loop in complex with AP2 suggests that M168L170 occupies a pocket in the AP2 σ2 subunit. Our data suggest a new model in the Nef-AP2 interaction in which the hydrophobic region in the Nef C-loop with the dileucine (L164L165) motif and M168L170 motif binds to AP2(σ2), while the acidic motif E174 and D175 binds to AP2(α), which explains how Nef through the flexible loop connects CD4 to AP2 for constitutive CD4 downregulation." @default.
• W2074443822 created "2016-06-24" @default.
• W2074443822 creator A5009434832 @default.
• W2074443822 creator A5011148019 @default.
• W2074443822 creator A5019520558 @default.
• W2074443822 creator A5030970923 @default.
• W2074443822 creator A5040387494 @default.
• W2074443822 creator A5063147878 @default.
• W2074443822 date "2013-04-01" @default.
• W2074443822 modified "2023-10-08" @default.
• W2074443822 title "Identification of a Novel Binding Site Between HIV Type 1 Nef C-Terminal Flexible Loop and AP2 Required for Nef-Mediated CD4 Downregulation" @default.
• W2074443822 cites W119567604 @default.
• W2074443822 cites W1674854702 @default.
• W2074443822 cites W1964958542 @default.
• W2074443822 cites W1977633077 @default.
• W2074443822 cites W1977745370 @default.
• W2074443822 cites W1980374511 @default.
• W2074443822 cites W1981951679 @default.
• W2074443822 cites W1989336397 @default.
• W2074443822 cites W1990719919 @default.
• W2074443822 cites W1996408830 @default.
• W2074443822 cites W1999613945 @default.
• W2074443822 cites W2005597401 @default.
• W2074443822 cites W2007845379 @default.
• W2074443822 cites W2014818809 @default.
• W2074443822 cites W2016409733 @default.
• W2074443822 cites W2029820720 @default.
• W2074443822 cites W2042556347 @default.
• W2074443822 cites W2047951231 @default.
• W2074443822 cites W2057567758 @default.
• W2074443822 cites W2070335320 @default.
• W2074443822 cites W2077704737 @default.
• W2074443822 cites W2086264258 @default.
• W2074443822 cites W2090839113 @default.
• W2074443822 cites W2100358892 @default.
• W2074443822 cites W2104927620 @default.
• W2074443822 cites W2112612176 @default.
• W2074443822 cites W2118276643 @default.
• W2074443822 cites W2121372252 @default.
• W2074443822 cites W2133715451 @default.
• W2074443822 cites W2135372551 @default.
• W2074443822 cites W2145045679 @default.
• W2074443822 cites W2145410464 @default.
• W2074443822 cites W2147439138 @default.
• W2074443822 cites W2150710995 @default.
• W2074443822 cites W2168770860 @default.
• W2074443822 cites W2301161050 @default.
• W2074443822 doi "https://doi.org/10.1089/aid.2012.0286" @default.
• W2074443822 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3903168" @default.
• W2074443822 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23151229" @default.
• W2074443822 hasPublicationYear "2013" @default.
• W2074443822 type Work @default.
• W2074443822 sameAs 2074443822 @default.
• W2074443822 citedByCount "11" @default.
• W2074443822 countsByYear W20744438222013 @default.
• W2074443822 countsByYear W20744438222014 @default.
• W2074443822 countsByYear W20744438222016 @default.
• W2074443822 countsByYear W20744438222019 @default.
• W2074443822 countsByYear W20744438222020 @default.
• W2074443822 countsByYear W20744438222021 @default.
• W2074443822 countsByYear W20744438222023 @default.
• W2074443822 crossrefType "journal-article" @default.
• W2074443822 hasAuthorship W2074443822A5009434832 @default.
• W2074443822 hasAuthorship W2074443822A5011148019 @default.
• W2074443822 hasAuthorship W2074443822A5019520558 @default.
• W2074443822 hasAuthorship W2074443822A5030970923 @default.
• W2074443822 hasAuthorship W2074443822A5040387494 @default.
• W2074443822 hasAuthorship W2074443822A5063147878 @default.
• W2074443822 hasBestOaLocation W20744438222 @default.
• W2074443822 hasConcept C104292427 @default.
• W2074443822 hasConcept C104317684 @default.
• W2074443822 hasConcept C127561419 @default.
• W2074443822 hasConcept C139770010 @default.
• W2074443822 hasConcept C143065580 @default.
• W2074443822 hasConcept C167625842 @default.
• W2074443822 hasConcept C170493617 @default.
• W2074443822 hasConcept C183786373 @default.
• W2074443822 hasConcept C195687474 @default.
• W2074443822 hasConcept C2779358239 @default.
• W2074443822 hasConcept C2779921545 @default.
• W2074443822 hasConcept C28005876 @default.
• W2074443822 hasConcept C51639874 @default.
• W2074443822 hasConcept C55493867 @default.
• W2074443822 hasConcept C62478195 @default.
• W2074443822 hasConcept C86803240 @default.
• W2074443822 hasConcept C95444343 @default.
• W2074443822 hasConceptScore W2074443822C104292427 @default.
• W2074443822 hasConceptScore W2074443822C104317684 @default.
• W2074443822 hasConceptScore W2074443822C127561419 @default.
• W2074443822 hasConceptScore W2074443822C139770010 @default.
• W2074443822 hasConceptScore W2074443822C143065580 @default.
• W2074443822 hasConceptScore W2074443822C167625842 @default.
• W2074443822 hasConceptScore W2074443822C170493617 @default.
• W2074443822 hasConceptScore W2074443822C183786373 @default.
• W2074443822 hasConceptScore W2074443822C195687474 @default.
• W2074443822 hasConceptScore W2074443822C2779358239 @default.
• W2074443822 hasConceptScore W2074443822C2779921545 @default.
• W2074443822 hasConceptScore W2074443822C28005876 @default.

• next